In-Vitro Anti-Bacterial, Phytochemical and Antioxidant Activity of Gezawa (Withania somnifera (L.) Dunal) Extracts Against Human Pathogenic Bacteria

Document Type : Research Paper

Authors

Department of Biotechnology, College of Natural and Computational Science, Wolkite University, Wolkite Ethiopia

Abstract

Withania somnifera (L.) Dunal is frequently used as traditional medicine for the treatment of numerous diseases. This study was aimed to assess bioactive compounds, antimicrobial activities and antioxidant studies on different part of W. somnifera. The plant parts were shaded dried and grinded to powders for biological activities. The ethanol, methanol and chloroform extracts were tested for antimicrobial activities. The extracts were subjected to a test of their antimicrobial properties by paper disc diffusion method. Minimum inhibitory concentrations (MICs) of the plant extracts against selected pathogens were also assessed using the agar dilution method. The highest potential was observed from methanol extract of the stem bark and ethanol extract of the leafagainst Staphylococcus aurous with zone of inhibition 15.02 and 13.22 mm, respectively.Alkaloids, steroids, saponins, tannins, reducing sugars, phlobatanins, flavonoids, terpenoids, cardiac glycosides, anthraquinone, emodins and coumarins are result of phytochemical assessment. Strong antioxidant effect of free radical scavenging activity was recorded at 100µg/ml concentration for ethanol stem and root, methanol leaf extracts (72.22%, 80.84%, 74.24%) respectively. Chloramphenicol and DMSO were used as positive and negative controls. W. somnifera could show powerful antimicrobial and antioxidant properties.

Keywords

References

1. Doughari, JH, AM. El-mahmood and I. Tyoyina. Antimicrobial activity of leaf extracts of Senna obstusifolia. African J Pharmacy Pharmaco. 2008;2:344-346.
2.  Abouzid SF, Bassuony AA, Nasib A, Khan S, Qureshi J, Choudhary MI.  Withaferin a production by root cultures of withania coagulans. 2010, pp. 762-769.
3. Tegos G, Frank R, Stermitz L, Olga Kim L. Multidrug Pump Inhibitors Uncover Remarkable Activity of Plant Antimicrobials Antimicrobial Agents and Chemotherapy. 2002;46:3133-3141.
4. Selvamohan T, Ramadas VS, Shibla SK. Antimicrobial activity of selected medicinal plants against some selected human pathogenic bacteria. J Pelagia Res Library. 2012;3:3374-3381.
5. Yirgashewa A, Ameha K, Dejene Z. In-vitro Antimicrobial Activities and Phytochemical Screening of Selected Plant Extracts against Some Medically and Agriculturally Important Pathogens. 2020;31:167-189.
6. Muskazli M, Nurhafiza Y, Nor A, Dalilah EN. Comparative study on the in vitro Antibacterial, Efficacy of Aqueous and Methanolic Extracts of Quercus infectoria Gall’s Aganist Cellulosimicrobium cellulans. J Biological Sci. 2008;4:47-50.
7. Samy RP, Ignacimuthu S. Antibacterial activity of some folklore medicinal plants used by tribals in Western Ghats o India. J Ethno Pharmacol. 2000;69:63-71.
8. Ahmad I, Beg AZ. Antimicrobial and on 45 phytochemical studies Indian Medicinal plants against multi-drug resistant human Pathogens Ethnopharmacol. 2017;4:113-123.
9. Nagham DR, Mahmood FI. Study effect of medical plant extracts in comparison with antibiotic against bacteria. J Sci Innovative Res. 2011;2:843-845.
10. Swaminathan. Int J of Chem Tech Res. 2019;12:218-222.
11. Devmurari, VP. and NP. Jivani. Annals of Biological Res. 2010;1:10-14.
12. Thamacian Arulappan M, John Britto S, Ruchmani K, Mohan Kumar R. Antimicrobial and Antifungal activities of W. Somnifera(1.f.) sond against human pathogens. Int J Development Res, 2015;5: 3858-3859.
13. Thamacin Arulappan S. Britto J, Igance Iindo K. GC-MS analysis of ormocarpumsennoides (willd) dc. (Leaf) and bauhinia tomentose 1. (Leaf). European J Phytochem Compounds Present in W. Somnifera (1.f.) sond. (Tuber), of Biomed Pharma Sci. 2014; 2: 324-342.
14. Al-Hindawi MK, Al-Khafaji SH, Abdul-Nabi MH. Antigranuloma activity of Iraqi Withania somnifera. J Ethnopharmaco. 1992;l37:113-116.
15. Anbalagan K, Sadique J.  Role of prostaglandins in acute phase proteins in inflammation. Biochem Med. 1984;31:236-245.
16.Anbalagan K, Sadique J. Influence of an Indian medicine (Ashwagandha) on acutephase reactants in inflammation Indian J Exp Biol. 1981;19:245-249.
17. Andallu B, Radhika B. Hypoglycemic, diuretic and hypocholesterolemic effect of winter cherry (Withania somnifera) root. Indian J Exp Biol. 2003;8:607-609.
18. Anonymous. Monograph: Withania somnifera. Altern Med, Rev. 2004;9:211-214.
19. Bhattacharya SK, Muruganandam AV. Adaptogenic activity of Withania somnifera: an experimental study using a rat model of chronic stress. Pharmacol Biochem Behav. 2003;75:547-555.
20. Dhuley JN. Nootropic-like effect of Ashwagandha (Withania somnifera). Phytother, Res. 2001;15:311-318.
21. Grigorescu M, E, Stanescu U. New data referring to chemistry of Withania somnifera species. Rev Med Chir Soc Med Nat Iasi. 1990;94:385-387.
22. Ghias Uddin, Sumaira Gul and Abdur Rauf. Preliminary Phytochemical Screening, in vitro Antimicrobial and Antioxidant Evaluation of Withania somnifera Dunal. World Applied Sci J. 2013;27:562-565.
23. Sharma H, Parihar L, Parihar P. Review on cancer and anticancerous properties of some medicinal plants. J  Med Plants Res. 2011;5:1818-1835.
24. Joy P, Thomas J, Mathew S, Skaria B. Medical Plants. Page 55. Aromatic and Kerala Medicinal Plants Research Station, Kerala Agricultural University, India. 1998.
25. Chaurasia  S, Panda S, Kar  A. Withania  somnifera  Root  Extract  in  The Regulation  of  Lead-Induced Oxidative Damage  in  Male  Mouse. Pharmacological Res. 2000; 41: 6.
26. Bhatia R, Narain JP. The growing challenge of antimicrobial resistance in the South East Asia Region-are us losing the battle? Indian J Med Res. 2010; 132: 482-486.
27. Coates A, Hu Y, Bax R, Page C.  The future challenges facing the development of new antimicrobial drugs. Nature Reviews Drug Discovery. 2002;1:895-910.
28. Giamarellou H. Multidrug-resistant Gram-negative bacteria:   how to treat and for how long. Int J Antimicrobial Agents. 2010;36: S50–S54.
29. Savithramma, N., M. lingarao and D. Suhrulatha. Screening of medicinal plant for secondary metabolites. Middle-East J. Sci. Res. 2011;8:579-584.
30. Boucher HW, Talbot GH, Bradley JS, et al. Bad bugs, no drugs: no ESKAPE! An update from the Infectious Diseases Society of America. Clinical Infectious Diseases. 2009;48:1-12.
31. Sofowora A. Medicinal Plants and Traditional Medicinal in Africa. Sunshine House, Ibadan, Nigeria, 1993, pp. 134-156.
32. Harborne JB. Phytochemical methods: A guide to modern technique of plant analysis, Champ man and Hall, London. 1998.
 
Journal of Medicinal plants and By-Products
Volume 10, Issue 1
April 2021
Pages 117-126

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