How Quantity of Bioactive Compounds of Zataria multiflora Differ Using Traditional or Modern Extraction Methods

Document Type : Research Paper

Authors

1 Research Center for Traditional Medicine and History of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

2 Department of Chemistry, College of Sciences, Shiraz University, Shiraz, Iran

3 Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran

4 Department of Plant Protection, College of Agriculture, Shiraz University, Shiraz, Iran

Abstract

For obtaining bioactive compounds, decoction of Zataria multiflora Boiss. (ZM) plant materials was interactively evaluated and compared with the modern method of ultrasound-assisted extraction (UAE). The interactive effects of several parameters including; liquid to solid (L/S) ratio, extraction time, extraction method, and plant material size were studied and optimized using the response surface methodology (RSM). Interpretation of the model outputs revealed that total phenolic content (TPC) of the resulting extracts is mostly affected by the interactive effect of plant material size and the extraction time, while rosmarinic acid (RA) content is only affected by the extraction method. Decoctions was found to have higher RA content (21.19 (mg/g E)) in comparison to UAE extractions (11.64 (mg/g E)), statistically significant at (p ≤ 0.05). In addition, UAE as the modern method of extraction showed no privilege over the conventional method in the case of RA and TPC extraction. However, results suggest higher efficiency of UAE compared to decoction for extraction of antioxidant compounds by water. Eventually, ZM decoction obtained by the optimal extraction conditions was chemically characterized for the first time by the aid of LC-MS/MS chromatography. Naringenin, which is almost exclusively found in Citrus fruits, besides luteolin-7-O-rutinoside and a natural derivative of citric acid were shown to be the major constituents of ZM decoction.

Keywords


  1. Sadat-Hosseini M, Farajpour M, Boroomand N, Solaimani-Sardou F. Ethnopharmacological studies of indigenous medicinal plants in the south of Kerman, Iran. J Ethnopharmacol. 2017;199:194-204.
  2. Rodrigues AM, Vieira Falé PL, Madeira P, Pacheco R, Florêncio MH, Ascensão L, Marques Serralheiro ML. Phenolic profile and biological activities of decoctions from Santolina impressa, a Portuguese endemic species. J Herbal Med. 2020;21:100335.
  3. Yang Y, Ross J. Theories and concepts in the composition of Chinese herbal formulas, In: Yang Y, Ross J (eds) Chinese Herbal Formulas. Churchill Livingstone. 2010.
  4. Both S, Strube J, Cravatto G. Mass transfer enhancement for solid–liquid extractions. Green extraction of natural products: theory and practice. 2015.
  5. Peng W.L, Mohd-Nasir H, Setapar S.H.M, Ahmad A, Lokhat D. Optimization of process variables using response surface methodology for tocopherol extraction from Roselle seed oil by supercritical carbon dioxide. Ind Crops Prod. 2020;143:111886.
  6. Shomali T. Zataria multiflora and Gastrointestinal Tract Disorders, In: Dietary Interventions in Gastrointestinal Diseases. Elsevier. 2019.
  7. Sajed H, Sahebkar A, Iranshahi M. Zataria multiflora Boiss.(Shirazi thyme) an ancient condiment with modern pharmaceutical uses. J Ethnopharmacol. 2013;145:686-698.
  8. Naghibi F, Mosaddegh M, Mohammadi Motamed M, Ghorbani A. Labiatae family in folk medicine in Iran: from ethnobotany to pharmacology. Iran J Pharm Res. 2010;4:63-79.
  9. Amirghofran Z, Ahmadi H, Karimi MH. Immunomodulatory activity of the water extract of Thymus vulgaris, Thymus daenensis, and Zataria multiflora on dendritic cells and T cells responses. J Immunoassay Immunochem. 2012;33:388-402.
  10. Berdowska I, Zieliński B, Fecka I, Kulbacka J, Saczko J, Gamian A. Cytotoxic impact of phenolics from Lamiaceae species on human breast cancer cells. Food Chem. 2013;141:1313-1321.
  11. Rajaei P, Mohamadi N. Ethnobotanical study of medicinal plants of Hezar mountain allocated in south east of Iran. Iran J Pharm Res. 2012;11:1153.
  12. Zargaran A, Zarshenas MM, Karimi A, Yarmohammadi H, Borhani-Haghighi A. Management of stroke as described by Ibn Sina (Avicenna) in the Canon of Medicine. Int J Cardiol. 2013;169:233-237.
  13. Izadiyan P, Hemmateenejad B, Francisco M, Rodríguez V.M, Izadiyan M. Metabolite fingerprinting and identification of potential quality markers of Zataria multiflora by a chemometric approach. J Iran Chem Soc. 2019;16:1-9.
  1. Zorić Z, Markić J, Pedisić S, Bučević-Popović V, Generalić-Mekinić I, Grebenar K, Kulišić-Bilušić T. Stability of Rosmarinic Acid in Aqueous Extracts from Different Lamiaceae Species after in vitro Digestion with Human Gastrointestinal Enzymes. Food Technol Biotechnol. 2016;54:97-102.
  2. Sarker SD, Latif Z, Gray AI. Natural Products Isolation. Springer Science Business Media. 2005;20.
  3. Herald TJ, Gadgil P, Tilley M. High‐throughput micro plate assays for screening flavonoid content and DPPH‐scavenging activity in sorghum bran and flour. ‎J Sci Food Agric. 2012;92:2326-2331.
  4. de Aguiar P F, Bourguignon B, Khots M, Massart D, Phan-Than-Luu R. D-optimal designs. Chemom Intell Lab Syst. 1995;30:199-210.
  5. Santos-Buelga C, Gonzalez-Manzano S, Dueñas M, Gonzalez-Paramas A.M. Extraction and isolation of phenolic compounds, In: Natural Products Isolation. 2012.
  6. Capelo-Martínez J.L. Ultrasound in chemistry: analytical applications. John Wiley and Sons. 2009.
  7. Belwal T, Ezzat S.M, Rastrelli L, Bhatt I.D, Daglia M, Baldi A, Devkota HP, Orhan IE, Patra J K, Das G. A critical analysis of extraction techniques used for botanicals: Trends, priorities, industrial uses and optimization strategies. TrAC, Trends Anal Chem. 2018;100:82-102.
  8. Rostagno M.A, Prado JM, Natural product extraction: principles and applications. vol 21. Royal Society of Chem. 2013.
  9. Zhang Q-W, Lin L-G, Ye WC. Techniques for extraction and isolation of natural products: A comprehensive review. ‎Chin Med. 2018;13:20.
  10. Rodríguez Seoane P, Flórez-Fernández N, Conde Piñeiro E, Domínguez González H. Chapter 6 - Microwave-Assisted Water Extraction, In: Water Extraction of Bioactive Compounds. Elsevier. 2017.
  11. Yolmeh M, Najafi MBH, Farhoosh R. Optimisation of ultrasound-assisted extraction of natural pigment from annatto seeds by response surface methodology (RSM). Food Chem. 2014;155:319-324.
  12. Oroian M, Escriche I. Antioxidants: Characterization, natural sources, extraction and analysis. Food Res Int. 2015;74:10-36.
  13. Hernández-Aquino E, Muriel P. Naringenin and the liver, In: Liver Pathophysiology. Elsevier. 2017.
  14. Joshi R, Kulkarni YA, Wairkar S. Pharmacokinetic, pharmacodynamic and formulations aspects of Naringenin: An update. Life Sci. 2018;215:43-56.