The Effect of Different Light Spectrum Ratios and Photosynthetic Photon Flux Density (PPFD) on Some Agronomic and Physiological Traits in Artemisia annua L.

Document Type : Research Paper

Authors

Department of Plant Production and Genetics, Faculty of Science and Agricultural Engineering, Razi University, Kermanshah, Iran

Abstract

In order to investigate the effect of the ratio of different light spectra on the growth and physiological traits of the medicinal plant Artemisia annua L., an experiment was conducted based on a randomized complete block design with three replications. Experimental treatments were six levels of different light wavelengths including control (base light: full spectrum), base light + ultraviolet spectrum, base light+blue light spectrum, base light + green light spectrum, base light + red light spectrum and base light + far-red light spectrum. The results showed that different light ratios influenced all the measured traits. Accordingly, the application of all light treatments significantly increased the dry weight of the Artemisia plant. Also, the light treatments had significant (P≤ 0.01) effects on plants height, contents of chlorophyll a and b, total chlorophyll, carotenoids and anthocyanin. The percentage and yield of plant essential oils were impressed significantly (P≤ 0.01) under the application of different light wavelengths. Although UV increased the content of the essential oil, it reduced the yield of the essential oil due to the reduction in the dry weight of plants. According to the results of this study, it can be concluded that a combination of base light with blue light can increase the biomass yield as well as percentage and yield of essential oils of Artemisia, compared to control.

Keywords

Main Subjects

References

  1. Weathers P.J., Elkholy S., Wobbe KK. The biosynthetic pathway and its regulation in Artemisia annua, a terpenoid rich species, In Vitro Cell. Dev. Biol.: Plant. 2006;42:309-317.
  2. Mannan A., Ahmed I., Arsad W., Asim M., .FQureshi R.A., Hussain I., Mirza B. Survey of Artemisinin production by diverse Artemisia species in northern Pakistan, Malar. 2010;3099:310.
  3. Liu C., Zhao Y., Wang Y. Artemisinin: current state and perspectives for Biotechnological production of an antimalarial drug, Appl. Microbiol. Biotechnol. 2006;72:11-20.
  4. Mutabingwa T.K. Artemisinin-based combination therapies (ACTs): best hope for malaria treatment but inaccessible to the needy, Acta Trop. 2005;95:305-315.
  5. Avery M.A., Chong W.K., Jennings M., White C. Stereoselective total synthesis of (+)-artemisinin, the antimalarial constituent of Artemisia annua L. J Am. Chem. Soc. 1992;114:974-979.
  6. Chen D.H., Ye H.C., Li G.F. Expression of a chimeric farnesyl diphosphate synthase gene in Artemisia annua L. transgenic plants via Agrobacterium tumefaciens-mediated transformation, Plant Sci. 2000;155:179-185.
  7. Ro D.K., Paradise E.M., Ouellet M., Fisher K.J., Newman K.L., Ndungu J.M., Ho K.A., Eachus R.A., Ham T.S., Kirby J., Chang M.C.Y., Withers S.T., Shiba Y., Sarpong R., Keasling J.D. Production of the antimalarial drug precursor artemisinic acid in engineered yeast, Nature. 2006;440:940-943.
  8. Avercheva O.V., Berkovich Y.A., Erokhin A.N., Zhigalova T.V., Pogosyan S.I., Smolyanina S.O. Growth and photosynthesis of Chinese cabbage plants grown under light-emitting diode-based light source. Russ J Plant Physiol. 2009;56:14-21.
  9. Fukuda N., Fujitan M., Ohta Y., Sase S., Nishimura S., Ezura H. Directional blue light irradiation triggers epidermal cell elongation of abaxial side resulting in inhibition of leaf epinasty in geranium under red light condition. Sci Hortic. 2008;115:176-182.
  10. Nishioka N., Nishimura T., Ohyama K., Sumino M., Malayeri S., Goto E., Inagaki N., Morota T. Light Quality Affected Growth and Contents of Essential Oil Components of Japanese Mint Plants; International Workshop on Greenhouse Environmental Control and Crop Production in Semi-Arid Regions: Tucson, AZ, USA; 2008; pp. 431-436.
  11. Asami D.K., Hong Y.J., Barrett D.M., Mitchell A.E. Comparison of the total phenolic and ascorbic acid content of freeze-dried and air-dried marionberry, strawberry, and corn grown using conventional, organic, and sustainable agricultural practices. J Agric Food Chem. 2003;51:1237-1241.
  12. Croteau R., Kutchan T.M., Lewis N.G. Natural products (secondary metabolites). Biochem Mol Biol Plants. 2000;24:1250-1319.
  13. Croteau R., Kutchan T.M., Lewis N.G. Natural products (secondary metabolites). Biochem. Mol Biol. Plants. 2000;24:1250-1319.
  14. Tanaka R., Tanaka A. Tetrapyrrole biosynthesis in higher plants. Plant Biol. 2007;58:321-346.
  15. Hoober J.K., Eggink L.L. Assembly of light-harvesting complex II and biogenesis of thylakoid membranes in chloroplasts. Photosynth Res. 1999;61:197-215.
  16. Marsac N.T., Houmard J. Adaptation of cyanobacteria to environmental stimuli: new steps towards molecular mechanisms. FEMS Microbiol Rev. 1993;1:119-189.
  17. Wang H., Gu M., Cui J.X., Shi K. Effects of light quality on CO2 assimilation, chlorophyll-fluorescence quenching, expression of Calvin cycle genes and carbohydrate accumulation in Cucumis sativus. J Photochem Photobiol B. 2009;96:30-37.
  18. Poudel P.R., Kataoka I., Mochioka R. Effect of red-and blue light- emitting diodes on growth and morphogenesis of grapes. Plant Cell Tiss Org. 2008;2:147-153.
  19. Kurilcik A., Canova M.R., Dapkuniene S., Zilinskaite S., Kurilcik G. In vitro culture of Chrysanthemum plantlets using lightemitting diodes. Cent Eur J Biol. 2008;2:161-167.
  20. Sood S., Gupta V., Tripathy B.C. Photoregulation of the greening process of wheat seedlings grown in red light. Plant Mol Biol. 2005;59:269-287.
  21. Nadernejad N.A., Ahmadimoghadam J., Hossyinifard S Poorseyedi S. Study of the rootstock and cultivar effect in PAL activity, production of phenolic and flavonoid compounds on flower, leaf and fruit in Pistachio (Pistacia vera L.). Iranian Journal of Plant Biology. 2013;5:95-109.
  22. Whitelam G., Halliday K. Light and Plant Development. Blackwell Publishing, Oxford, p.313. with UV-B, J. Plant Physiol. 2007;147:589-592.
  23. Abinaya M., Prabhakaran S., Nur H., Chung H., Byoung R. Blue LED Light Enhances Growth, Phytochemical Contents, and Antioxidant Enzyme Activities of Rehmannia glutinosa Cultured in Vitro. Environ. Biotechnol. 2015;56:105-113.
  24. Sakalauskien E.S., Samuolien G., Brazaityt A. Supplementary UV-B irradiation effects on basil (Ocimum basilicum L.) growth and phytochemical properties. J. Food Agric. Environ. 2012;10:342-346.
  25. Boccalandro H.E., Giordano C.V., Ploschuk E.L., Piccoli P.N., Bottini R., Casal J.J. Phototropinsbut notcrypto chromes mediate the blue light-specific promotion of stomatal conductance, while both enhance photosynthesis and transpiration under full sunlight. Physio Plant. 2012;158:1475-84.
  26. Cristiane P.V., Marcos V., Leal-costa E., Schwart T., Tavares R., Machado K., Celso L. Light spectra affect the morphoanatomical and chemical feautures of clonal Phyllanthus tenellus Roxb grown in vitro. Soc. 2015;114:69-119.
  27. Li H., Tang M., Xu M., Liu Z.G., Han X.Y. Effects of different light sources on the growth of non-heading Chinese cabbage (Brassica campestris L.). J Agric Sci. 2012;4:262-273.
  28. Lillo C., Appenroth K.J. 2001. Light regulation of nitrite reductase in higher plants: which photoreceptors are involved? .Plant Biology. 2012; 3: pp. 455-465.
  29. Terfa M.T., Poudel M.S., Roro A.G., Gislerod H.R., Olsen J.E., Torre S. Light emitting diodeswith a high proportion of blue light affects external and internal quality param-eters of pot roses differently than the traditional high pressure sodium lamp. Acta Hortic. 2012a;956:635-642.
  30. Candan N., Tarhan L. Changes in chlorophyll-carotenoid contents, cytokinins. Plant growth regulation. 2003;32:359-567.
  31. Kopsell D.A., Kopsell D.E., Lefsrud M.G., Curran-Celentano J., Dukach L.E. Variation inlutein, carotene, and chlorophyll concentrations among Brassica oleraceacultigens and seasons. HortScience. 2004;39:361-364.
  32. Mackerness S.A.H. Plant responses to ultraviolet-B (UV-B: 280-320 nm) stress: What are the key regulators?. Plant Growth Regul. 2000;32:27-39.
  33. Singh N., Luthra R., Sangwan R. Oxidative pathways and essential oil tissue-specific expression of an anionic peroxidase in zucchini. Plant Physiology. 1990b;26:2129-2136.
  34. Coley P.D. Costs and benefits of defense by tannins in a neotropical tree complex patterns of terpenoid gene expression. Biotechnol Bioeng. 1986;83:653-667.
Journal of Medicinal plants and By-Products
Volume 11, Issue 2
September 2022
Pages 139-147

Files

Share

How to cite

Statistics