Evaluation of Antioxidant and Antimicrobial Activity of Some Medicinal Plant Extracts on Escherichia coli Isolated from Poultry Feces

Document Type : Research Paper

Authors

1 Research Department of Agronomy and Plant Breeding, Agricultural Research Institute, University of Zabol, Zabol, Iran

2 Research Center of Medicinal Plants, University of Sistan and Baluchestan, Zahedan, Iran

3 Department of Food Science and Technology, Zahedan University of Medical Sciences, Zahedan, Iran

Abstract

Continuous and indiscriminate use of chemical drugs causes an important phenomenon of resistance to microorganisms and consequently, the effect of the drugs is weakened or neutralized. On the other hand, it has been reported that many plant essential oils have a significant inhibitory effect on pathogenic microorganisms, so, this study aimed to evaluate the antioxidant and antimicrobial activity of several plant extracts on Escherichia coli isolated from poultry feces. Cichorium intybus L., Hypericum perforatum L., Lavandula angustifolia Mill., and Thymus vulgaris L. from Shahrekord were collected and determined in the botanical laboratory of University of Zabol. To prepare the ethanolic extract, 40 gr of dried leaves of plants used in 400 cc of ethanol were used. The various strains of E. coli used were isolated from poultry feces by biochemical, bacteriological, and growth tests as well as standard tests. Determination of the free radical trapping activity was perfomed bydiphenylpicryl hydroxyl, and then the antimicrobial effects were investigated by diffusion method in Müller Hinton agar medium using 6 mm paper discs according to Bauer and Kirby instructions. Statistx ver10 software was used for statistical calculations. Mean comparisons were performed using the least significant difference (LSD) test. The results showed an increasing trend of the antioxidant activity of the extracts with increasing the concentration of plant extracts. The interaction of plant extract and the amount of extract in trapping free radicals showed that the highest antioxidant activity at low concentrations of the extract (16 and 32 μg/ml) was observed in the chicory extract following by licorice extract, but it licorice extract showed the highest activity at high concentration (64 μg/ml). In general, H. perforatum L. was the most effective plant in trapping free radicals. The lowest MIC of H. perforatum L. was 3.1 ppm, which was unilaterally inhibited at this concentration. H. perforatum L. and chicory had the highest (5.38) and lowest (2.23) diameter halos of inhibition of E. coli growth. Considering the side effects of chemical drugs and antibiotics as well as the potential effect of medicinal plant extracts used, especially H. perforatum L. on E. coli, compared to Cefazolin, it is recommended to use H. perforatum L. in inhibiting growth of E. coli.

Keywords

References

  1. Valizadeh M., Beigomi M., Fazeli-Nasab B. Antibacterial and Anti biofilm effects of ethanol and aceton leaf extract of Momordica charantia and Tecomella undulata against Acinetobacter baumannii. International J Advanced Biological and Biomedical Res. 2020;8:403-418. https://doi.org/10.33945/sami/ijabbr.2020.4.6
  2. Ismail S.M. Cholinesterase and Aliesterase as a Natural Enzymatic Defense against Chlorpyrifos in Field Populations of Spodoptera Littoralis (Boisdüval, 1833) (Lepidoptera, Noctüidae). J Plant Bioinform Biotech. 2021;1:41-50. https://doi.org/10.22034/jpbb.2021.288332.1007
  3. Naderi D., Jami R., Rehman F.U. A Review of RNA Motifs, Identification Algorithms and their Function on Plants. J Plant Bioinform. Biotech. 2021;1:28-40. https://doi.org/10.22034/jpbb.2021.271442.1001
  4. Fooladvand Z., Fazeli-nasab B. Antibacterial activities of Stachys lavandulifolia extract against eight bacteria. J Herbal Drugs (An International J on Medicinal Herbs). 2014;5:13-18.
  5. Fazeli-nasab B., Moshtaghi N., Forouzandeh M. Effect of Solvent Extraction on Phenol, Flavonoids and Antioxidant Activity of some Iranian Native Herbs. Scientific J Ilam University of Medical Sci. 2019;27:14-26. https:doi.org/10.29252/sjimu.27.3.14
  6. Fazeli-Nasab B., Rahnama M., Mazarei A. Correlation between Antioxidant Activity and Antibacterial Activity of Nine Medicinal Plant Extracts. J Mazandaran Univ Med Sci. 2017;27:63-78.
  7. Naddaf M.E., Rabiei G., Ganji Moghadam E., Mohammadkhani A. In vitro Production of PPV-free Sweet cherry (Prunus avium cv. Siahe-Mashhad) by Meristem culture and micro-grafting. J Plant Bioinform. Biotech. 2021;1:51-59. https://doi.org/10.22034/jpbb.2021.282382.1005
  8. Fazeli-Nasab B., Rahnama M., Shahriari S. The antimicrobial properties of hydro-alcoholic extracts of 29 medicinal plants on E. coli and Staphylococcus aureus New Findings in Veterinary Microbiology. 2018;2: 1-15.
  9. Fazeli-Nasab B., Yazdanpour Z. Antimicrobial effects of extract of Citrullus colocynthis and Teucrium polium on some Bacteria. New Findings in Veterinary Microbiology. 2020;3: 1-10. http://nfvm.uoz.ac.ir/article_113940.html
  10. Fazeli-Nasab B., Rahmani A.F., Khajeh H. Effects of culture medium and plant hormones in organogenesis in olive (CV. Kroneiki). J Plant Bioinform. Biotech. 2021;1:1-13. https://doi.org/10.22034/jpbb.2021.268638.1000
  11. Khajeh H., Fazeli F., Mazarie A. Effects of Culture Medium and Concentration of Different Growth Regulators on Organogenesis Damask rose (Rosa damascena Mill). J Plant Bioinform. Biotech. 2021;1:14-27. https://doi.org/10.22034/jpbb.2021.276335.1004
  12. Wach A., Pyrzyńska K., Biesaga M. Quercetin content in some food and herbal samples. Food Chem. 2007;100:699-704. https://doi.org/10.1016/j.foodchem.2005.10.028
  13. Forouzandeh M., Mohkami z., Fazeli-Nasab B. Evaluation of Biotic Elicitors Foliar Application on Functional Changes, Physiological and Biochemical Parameters of Fennel (Foeniculum vulgare). Int. J Plant Prod. 2019;25: 49-65. https:doi.org/10.22069/jopp.2018.14077.2262
  14. Hossein-Abadi M., Mehrabi A.A., Etminan A.R., Fazeli-Nasab B. Studying of callus induction and plant regeneration of medicinal plants Valeriana Offinalis. J Medicinal Plants Biotechnology. 2015;1:20-34.
  15. Najafi Momen R., Torabi Goudarzi M., Bahonar A., Akbari H., Darabi M. Clinical Evaluation of the Effect of Myrtle Oil on the Oral Lesions of FMD in Cattle. [Research]. J Medicinal Plants. 2011;2:135-141 [Farsi].
  16. Al-saimary L.E., Bakr S.S., Jaffar T., Al-saimary A.E., Al-Muosawi R. Effect of some plant extracts and antibiotics on Psendo minas aeruginosa isolated from various burn cases. Saudi Medical J. 2002;23:802-805.
  17. Vahidi H., Kamalinejad M., Sedaghati N. Antimicrobial properties of Croccus sativus L. Iranian J Pharmaceutical Res. 2010;1:33-35.
  18. Larrán S., Ringuelet J.A., Carranza M.R., Henning C.P., Ré M S., Cerimele E.L., Urrutía M.I. In vitro fungistatic effect of essential oils against Ascosphaera apis. J Essential Oil Res. 2001;13:122-124. https://doi.org/10.1080/10412905.2001.9699633
  19. Sokmen A., Sokmen M., Daferera D., Polissiou M., Candan F., Ünlü M., Akpulat H.A. The in vitro antioxidant and antimicrobial activities of the essential oil and methanol extracts of Achillea biebersteini (Asteraceae). Phytotherapy Res. 2004; 18:451-456.
  20. Hadizadeh H., Mohebodini M., Esmaeilpoor B., Chamani E. Studies on Callus Induction and Regeneration of Medicinal Plant Chicory (Cichorium intybus) from Leaf and Petiole Explants. J Horticulture Science. 2015;29:621-630. https://doi.org/10.22067/jhorts4.v29i4.32672
  21. Babaei Z., Solouki M., Fazeli-Nasab B. Investigating The Effect of Biological and non-Biological Elicitor on Expression of Hyp-1 Gene in Hypericum perforatum. Modern Genetics. 2018;13: 543-549. Article Code: MGJ-17-B-00487
  22. Yaghoobi K., Kaka G.R., Davoodi Sh., Ashayeri H. Therapeutic effects of Lavandula angustifolia. [Review Article]. J Gorgan University of Medical Sci. 2016;17:1-9.
  23. Hosseinzadeh F., Sadeghieh Ahari S., Mohammadian-erdi A. Survey the Antibiotics Prescription by General Practitioners for Outpatients in Ardabil City in 2013. J Ardabil University of Medical Sci. 2016;16:140-150.
  24. Nadjafi F., Koocheki A., Honermeier B., Asili J. Autecology, ethnomedicinal and phytochemical studies of Nepeta binaludensis Jamzad a highly endangered medicinal plant of Iran. Journal of Essential Oil Bearing Plants. 2009;12:97-110. https://doi.org/10.1055/s-2007-987389
  25. Shafiee P., SHOJA A.S., Charkhabi A.H. Biodegradation of polycyclic aromatic hydrocarbons by aerobic mixed bacterial culture isolated from hydrocarbon polluted soils. Iranian J Chem And Chem Engin (IJCCE). 2006;25:73-78 (In Persian).
  26. Khodadadi S., Mahdinezhad N., Fazeli-Nasab B., Heidari M.J., Fakheri B., Miri A. Investigating the Possibility of Green Synthesis of Silver Nanoparticles Using Vaccinium arctostaphlyos Extract and Evaluating Its Antibacterial Properties. BioMed Res International. 2021. Article ID: 5572252. https://doi.org/10.1155/2021/5572252
  27. Bauer A., Kirby W., Sherris J.C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. American J Clinical Pathology. 1966;45:493-496.
  28. Wikler M.A. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard. CLSI (NCCLS). 2006;26:M7-A7. NII Article ID (NAID): 20001404762
  29. Kiehlbauch J.A., Hannett G.E., Salfinger M., Archinal W., Monserrat C., Carlyn C. Use of the National Committee for Clinical Laboratory Standards guidelines for disk diffusion susceptibility testing in New York state laboratories. J Clinical Microbiology. 2000;38:3341-3348.
  30. Owuama C.I. Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using a novel dilution tube method. African J Microbiology Res. 2017; 11:977-980. https:doi.org/10.5897/AJMR2017.8545
  31. Lambert R., Pearson J. Susceptibility testing: accurate and reproducible minimum inhibitory concentration (MIC) and non-inhibitory concentration (NIC) values. J Appl Microbiol. 2000;88: 784-790. https://doi.org/10.1046/j.1365-2672.2000.01017.x
  32. Ebrahimzadeh M.A., Hosseinimehr S.J., Hamidinia A., Jafari M. Antioxidant and free radical scavenging activity of Feijoa sallowiana fruits peel and leaves. Pharmacology online. 2008;1: 7-14.
  33. Statistix R. Statistix 10 Analytical Software. Tallahassee, FL USA. 2013.
  34. Fazeli-Nasab B., Mirzaei N. Evaluation of total phenol and flavonoid content in a wide range of local and imported plants. Scientific J Ilam University of Medical Sci. 2018;26: 141-154. http://doi.org/10.29252/sjimu.26.2.141
  35. Rahnama M., Fazeli Nasab B., Mazarei A., Shahriari S. Evaluation of antimicrobial activity hydro alcoholic extract of some medicinal herbs against a range of Gram-positive and gram-negative bacteria. New Findings in Veterinary Microbiology. 2018; 2:1-19.
  36. Ghasemi Pirbalouti A., Jahanbazi P., Enteshari S., Malekpoor F., Hamedi B. Antimicrobial activity of some Iranian medicinal plants. Archives of Biological Sci. 2010;62: 633-641.
  37. Amensour M., Bouhdid S., Fernandez-Lopez J., Idaomar M., Senhaji N.S., Abrini J. Antibacterial activity of extracts of Myrtus communis against food-borne pathogenic and spoilage bacteria. International J Food Properties. 2010;13: 1215-1224.
  38. Salvagnini L.E., Oliveira J.R.S., Santos L E d, Moreira R.R.D., Pietro R.C.L. Evaluation of the antibacterial activity of Myrtus communis(Myrtaceae) leaves. Revista Brasileira de Farmacognosia. 2008; 18:241-244.
  39. Afshar-Mohammedan M., Kordi S., Mashhadi-Nejad A. Antibacterial activity of stigma and petal of different species of saffron (Crocus Spp.). J Cellular and Molecular Res (Iranian J Biology). 2016;29: 265-273.
  40. Gandomi Nasrabadi H., Azami Sarokelaei L., Misaghi A., Abbaszadeh S., Shariatifar N., Tayyar Hashtjin N. Antibacterial effect of aqueous and alcoholic extracts from petal of saffron (Crocus sativus) on some foodborne bacterial pathogens. J Medicinal Plants. 2012;2: 189-196.
  41. Razzaghi R., Nourbakhsh R., Hemmati Kakhaki A., Saberi Najafi M. Antimicrobial effect of saffron. 3rd national congress on saffron, Iran [Farsi]. 2003.
  42. Tayel A.A., El Tras W.F. Possibility of fighting food borne bacteria by egyptian folk medicinal herbs and spices extracts. J Egypt Public Health Assoc. 2009;84:21-32.
  43. Ghaderi A.A., Fakheri B., Mahdinezhad N., Saeedi S. Assessment of the Antimicrobial Effects of the Thymus Vulgaris Ethanol Extract Against Human Pathogenic Bacteria. Journal of Sabzevar University of Medical Sci. 2016;23: 756-761. https://doi.org/10.21859/sums-2305756
  44. Bajalan I., Rouzbahani R., Ghasemi-Pirbalouti A., Maggi F. Chemical composition and antibacterial activity of Iranian lavandula× hybrida. Chem & Biodiversity. 2017;14:e1700064. https://doi.org/10.1002/cbdv.201700064
  45. Moon T., Wilkinson J., Cavanagh H. Antibacterial activity of essential oils, hydrosols and plant extracts from Australian grown Lavandula spp. International J Aromatherapy. 2006;16:9-14. https://doi.org/10.1016/j.ijat.2006.01.007.
  46. Moussi Imane M., Houda F., Said Amal A.H., Kaotar N., Mohammed T., Imane R, Farid H. Phytochemical Composition and Antibacterial Activity of Moroccan Lavandula angustifolia J Essential Oil Bearing Plants. 2017; 20: 1074-1082. https://doi.org/10.1080/0972060X.2017.136300.
Journal of Medicinal plants and By-Products
Volume 11, Issue 2
September 2022
Pages 265-275

Files

Share

How to cite

Statistics