Karyotype Analysis of Populations in Five Satureja Species from Iran

Document Type : Research Paper


1 Department of Horticulture Science and Agronomy, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Biotechnology, Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran

3 Department of Medicinal plants, Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran

4 National Institute of Genetic Engineering and Biotechnology, Tehran, Iran


A karyological study of five taxa (15 populations) of the genus Satureja L. from different geographic origins is presented. The haploid levels were different among species (n= 12 and 15). The work deals with chromosome number and morphometry. We found two of the usual basic chromosome numbers in this study for satureja species, x= 12 and x= 15. All populations were diploid and were located in 1B or 2B Stebbins classes. The karyotypic results showed the diversity among the species as mostly displayed median region (m) and submedian region (sm) and the chromosome lengths were determined between 0.60 µm in Satureja sahendica Bornm. (1041) to 2.27 µm in S. macrantha C.A.Mey. (3). Detailed karyotype analysis allows us to group the different populations and determine the relationships among them. It also pointed out the possibility of inter and intra-species cross to improve plants.


  1. Nixon K. Genetic diversity in Satureja genus, diversity of life.org (DOL), Cornell University. 2006. from http://www.Plantsystematics.org.
  2. Jamzad Z. Thymus and Satureja species of Iran, Research Institute of Forests and Rangelands, Tehran, Iran. 2009;1:171.
  3. Sefidkon F., Jamzad Z., Mirza M. Chemical variation in the essential oil of Satureja sahendica from Iran. Food Chem. 2004;88:325-328
  4. Sefidkon F, Jamzad Z. Essential oil analysis of Iranian Satureja edmondi and S. isophylla. Flavour Fragr. J 2006;21:230-233
  5. Khadivi-Khub A., Salehi-Arjmand H., Hadian J. Morphological and phytochemical variation of Satureja bachtiarica populations from Iran. Industrial Crops and Products. 2014;54:257-265
  6. Rechinger K. Flora Iranica, Labiatae, Akademische Druke- u.Verlagsanstalt. Graz Austria. 1982;150:479-480.
  7. Farsam H., Amanlou M., Radpour M.R., Salehinia A.N., Shafiee A. Composition of the essential oils of wild and cultivated Satureja khuzestanica Jamzad from Iran. Flavour Frag J. 2004;19:308-310.
  8. Hadian J., Mirjalili M.H., Kanani M.R., Salehnia A., Ganjipoor Chem P. Phytochemical and morphological characterization of Satureja khuzistanica Jamzad populations from Iran. Biodivers. 2011;8:902-915.
  9. Kasyani Aval M., Tabaei Aghdaei S.R., Sefidkon F., Jafari A.A., Eftekhari S.A. Study the morphology and essential oil content in two Satureja khuzistanica populations under Tehran climatic condition. Annals of Biological Res. 2012;3:975-978.
  10. Attar F., Einollahi N., Keyhani E., Keyhani J. Study on Superoxide Dismutase in Satureja hortensis L. Roots. Acta Hort. (ISHS). 2006;723:215-220.
  11. Hadian J., Azizi A., Fakhr Tabatabaei M., Naghavi M.R., Jamzad Z., Friedt W. Analysis of the genetic diversity and affinities of different Iranian Satureja species based on SAMPL markers. Planta Med. 2010;76:1927-1933.
  12. Bräuchler C., Meimberg H., Heubl G. New names in Old World Clinopodium L. - the transfer of the species of Micromeria sect. Pseudomelissa to Clinopodium. Taxon. 2006;55:977-981.
  13. Bräuchler C., Meimberg H., Abele T., Heubl G. Polyphyly of the genus Micromeria (Lamiaceae) - evidence from cpDNA sequence data. Taxon. 2005;54:639-650.
  14. Bräuchler C., Ryding O., Heubl G. The genus Micromeria (Lamiaceae), a synoptical update. Willdenowia. 2008;38:363-410.
  15. Saidi M., Movahedi K., Mehrabi A.A., Kahrizi D. Molecular genetic diversity of Satureja bachtiarica. Molecular Biology Reports. 2013;40:6501-6508.
  16. Zarei B., Kahrizi D., Sayfi T., Movahedi R., Ghaheri M., Kazemi E. The Antibacterial Effect of Alcoholic Extraction of Satureja bachtiarica on Four Bacterial Human Pathogens. Agric Biotechnology. 2017;8:81-86.
  17. Krogulevich R.E. Karyological analysis of the species of the flora of eastern Sayana. 19-48. In Malyschev L. I, Peschkova G. A, (eds.) Fl. Prebaikalya. Nauka, Novosibirsk. 1978.
  18. Lövkvist B., Hultgård U.M. Chromosome numbers in south Swedish vascular plants. Opera Bot. 1999;137:1-42.
  19. Markova M, Goranova V. Mediterranean chromosome number reports 5: 435-473. Fl. Medit. 1995;5:289-317.
  20. Irani P., Hesamzadeh Hejazi S.M., Tabaei Aghdaei S.R. Karyological study on four species of Satureja (Lamiaceae) in Iran. International J Biosciences. 2014;4:229-240.
  21. Markova M.L. Chromosome numbers of Bulgarian angiosperms. Fitologija. 1989;36:67-68.
  22. Shariat A., Karimzadeh G., Assareh M.H. Karyology of Iranian Endemic Satureja (Lamiaceae) Species. Cytologia. 2013;78:305-312.
  23. Boscaiu M., Riera J., Estrelles E., Güemes J. Números cromosomáticos de plantas occidetales, 827-848. Anales Jard. Bot. Madrid. 2000;58:163-164.
  24. Morton J.K. Chromosome numbers and polyploidy in the flora of Cameroon Mountain. Opera Bot. 1993;121:159-172.
  25. Ferakova V., Murin A. In Index to chromosome numbers of Slovakian flora. Part 4. Acta Fac. Rerum Nat. Univ. Comenianae, Bot. 1974;23:1-23.
  26. Reeves A., Tear J. MicroMeasure version 3.2. Colorado State University, USA. 2000. http://www.colostate.edu/Depts/Biology/MicroMeasure/
  27. Hesamzadeh Hejazi S.M., Rasouli M. Cytogenetic study of some species of Vetch Genus (Vicia sp.) in Iran. Iran. J Agric Sci. 2006;37:213-225.
  28. Javadi H., Hesamzadeh Hejazi S.M., Majnoon SH. B. Karyotipic studies of three Thymus (Lamiaceae) species and populations in Iran. Caryologia. 2009;62:316-325.
  29. Hesamzadeh Hejazi S.M., Ziaei Nasab M. Cytogenetic study on several populations of diploid species of Onobrychis in natural gene bank of Iran. Iran J. Rangelands for Plant Breed Genet Res. 2009;16:158-171.
  30. Hesamzadeh Hejazi S.M. Karyological study on three Cicer L. species (Fabaceae) in Iran. Asian J Cell Biol. 2011;6:97-104.
  31. Ghasemi E., Hesamzadeh Hejazi S.M. Karyological studies in different populations of Buxus hyrcana (buxaceae) in Iran. Iranian J botany. 2018;24:156-162.
  32. Soltanipoor M.A., Hesamzadeh Hejazi S.M., Jonoubi P. Karyotypic studies in eight populations of Zhumeria majdae Rech. f. & Wendelbo from Iran. Caryologia. 2017;70:222-228.
  33. Huziwara Y. Karyotype analysis in some genera of compositae. V III, Further studies on the chromosome of aster. Am. J. Bot. 1962;49:116-119.
  34. Romero Zarco C. A new method for estimating Karyotype asymmetry. Taxon. 1986;35:526-530.
  35. Lavania U.C., Srivastava S. A simple parameter of dispersion index that serves as an adjunct to karyotype asymmetry. J. Biosci. 1992;17:179-182.
  36. Stebbins G.L. Chromosomal evoluation in higher plants. Edward Arnold publisher LTd, London. 1971;216pp.
  37. Levan A.K., Sandberg A. Nomenclature for centrometric position on chromosomes. Hereditas.1964; 52:201-220.
  38. JMP. JMP/STAT for windows, version 3.1.2. SAS Institute Inc. 1995.
  39. STATISTI X.L. Statistical for windows, version 1.8. University of Western Australia. 2007. from http:// www.statistixl.com.
  40. SAS. SAS/STAT for Windows.Version 6.12 SAS Institute Inc., Cary, NC. USA. 1996
  41. Hesamzadeh Hejazi S.M., Ziaei Nasab M. Cytaxonomy of some Onobrychis (Fabaceae) species and populations in Iran. Caryologia. 2010;63:18-31.
  42. Kalvandi R., Hesamzadeh Hejazi S.M., Atri M., Mirza M., Jamzad Z., Safikhani K. Karyotype analysis among 10 populations of Thymus eriocalyx species in Iran. Ann. Biol Res. 2012;3:3916-3925.
  43. Salehi M., Hesamzadeh Hejazi S.M., Tabaei aghdaei S.R. Cytogenetic studies of two Dracocephalum (Lamiaceae) species and populations in Iran. Int J Biosci. 2014;4:100-108.