Essential Oil Diversity among Stachys laxa Boiss. & Buhse Populations in North of Iran

Document Type : Research Paper

Authors

1 Department of Horticulture, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran

3 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

4 Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahed University, Tehran, Iran

5 Medicinal Plants Research Center, Shahed University, Tehran, Iran

Abstract

Stachys laxa, Boiss. & Buhse a member of the Lamiaceae family, is a medicinal and aromatic that found in semi-cold and Mediterranean regions of northern Iran. The essential oil composition of Iranian pof S. laxa were evaluated in this study. The 16 populations of S. laxa were collected from North of Iran. The essential oils were extracted by hydrodistillation using Clevenger type and were analyzed by GC and GC/MS. Based on the essential oils analysis, 37 different chemical compounds were identified in this study within the sixteen populations of S. laxa. The results revealed that distinct differences in the content of compounds depending on region of sample collection. The main constituents of the identified essential oils were β-Elemene (1.1-18.7%), trans-Caryophyllene (0-13.2%), Germacrene D (1.1-46.6%), and Caryophyllene oxide from (0.3-32.3%). According to the GC/MS results, some components such as Germacrene A, Spathulenol, Germacrene D, and α-cadinol were the effective components to separate different populations of S. laxa. In general, the lowest and highest content of germacrene D was founded from 1.1 to 46.6% in the population of Kiasar (KSR), and Chalus (CHL), respectively. The S. laxa populations had high diversity in term of essential oil components, which could be considered in future studies.

Keywords

Main Subjects


  1. Ghahreman A. Plant Systematics. Tehran University Publication Center. 1994.
  2. Rechinger K., et al. Flora Iranica, Akademische Druck-U. Verlagsanstalt, Graz. 1982;150:108-216.
  3. Tundis R., Peruzzi L., and Menichini F. Phytochemical and biological studies of Stachys species in relation to Chemotaxonomy: a review. Phytochemistry. 2014;102:7-39.
  4. Lashgargahi Z., Shafaghat A. Volatile Constituents of Essential Oils Isolated from Fresh and Dried Stachys lavandulifolia and Stachys byzantina C. Koch. Two Lamiaceae from North-West Iran. J Essential Oil Bearing Plants. 2017;20:1302-1309.
  5. Khanavi M., et al. Comparison of the antioxidant activity and total phenolic contents in some Stachys species. African J Biotechnology. 2009;8.
  6. Skaltsa H.D., et al. Essential oil analysis and antimicrobial activity of eight Stachys species from Greece. Phytochemistry. 2003;64:743-752.
  7. Skaltsa H.D., et al. Composition and antibacterial activity of the essential oils of Stachys candida and chrysantha from southern Greece. Planta Medica. 1999;65:255-256.
  8. Mahzooni-Kachapi S., et al. The effect of altitude on chemical compositions and function of essential oils in Stachys lavandulifolia(Iran). Int J Med Arom Plants. 2014;4:107-116.
  9. Salimi F., Shafaghat A., Sahebalzamani H., Habibzadeh H. Analysis and Comparison of Chemical Composition of Essential Oils from Stachy byzantina C. Koch. Wet and Dried, Archives of Applied Sci Res. 2011;3:381-383.
  10. Semnani K.M., Akbarzadeh M., Changizi S. Essential Oils composition of Stachys byzantina, S. inflate, S. Lavandulifolia an S. laxa from Iran, Flavour and Fragrance J. 2006;21:300-303.
  11. Se S., and Mehregan I. Composition of the essential oil of Stachys laxa & Buhse. Iranian J Pharmaceutical Res. 2010;57-58.
  12. Salmaki Y., et al. A taxonomic revision of the genus Stachys (Lamiaceae: Lamioideae) in Iran. Botanical J the Linnean Society. 2012;170:573-617.
  13. Omidbaigi, R., Production and processing of medicinal plants. Vol. 2. Razavi Ghods Astan Publication. 2009;347.
  14. https://en.climate-data.org. 2019.
  15. Vokou D., Kokkini S., and Bessiere J.M. Geographic variation of Greek oregano (Origanum vulgare ssp. hirtum) essential oils. Biochemical Systematics and Ecology. 1993;21:287-295.
  16. , G., Ecological identification of Stachys and Nepeta species in Mazandaran (with emphasis on medicinal properties). J Plant Res. 2008.
  17. Kiashi F., Hadjiakhoondi A., Tofighi1 Z., Khanavi M., Ajani Y., Ahmadi Koulaei Sh., Yassa N. 2021. Compositions of Essential Oils and Some Biological Properties of Stachys laxa & Buhse and S. byzantina K. Koch, Res J Pharmacognosy (RJP). 2021;8:5-15.
  18. Nejadhabibvash F., et al. Effect of Harvesting Time on Content and Chemical Composition of Essential Oil from Stachys lavandulifolia Vahl (Lamiaceae). J Medicinal Plants and By-Product. 2018;7:181-187.
  19. Hajdari A., et al., Essential oil composition and antioxidant activity of Stachys sylvatica(Lamiaceae) from different wild populations in Kosovo. Natural Product Res. 2012;26:1676-1681.
  20. Renda G., et al. Volatile Constituents of three Stachys L. species from Turkey. Marmara Pharmaceutical J. 2017;21:278-285.
  21. Grujic‐Jovanovic S., et al. Composition and antibacterial activity of the essential oil of six Stachys species from Serbia. Flavour and Fragrance J. 2004;19:139-144.
  22. Bahadori MB, Zengin G, Dinparast L, Eskandani M. The health benefits of three hedgenettle herbal teas (Stachys byzantina, Stachys inflata, and Stachys lavandulifolia)-profiling phenolic and antioxidant activities. Eur J Integ Med. 2020;36:1-7.
  23. Alizadeh F., Ramezani M., Piravar Z. Effects of Stachys sylvatica hydroalcoholic extract on the ovary and hypophysis-gonadal axis in a rat with polycystic ovary syndrome. Middle East Fertil Soci J. 2020;25:1-7.
  24. Aghaei Y, Hossein Mirjalili M, Nazeri V. Chemical diversity among the essential oils of wild populations of Stachys lavandulifolia VAHL (Lamiaceae) from Iran. Chem Biodivers. 2013;10:262-273.
  25. Mann C., Staba E.J. Commercial Formulations of Chamomile. Herbs, spices, and medicinal plants: recent advances in botany, horticulture, and Pharmacology. 1992;1:235.
  26. Jerkovic I., et al. Chemical composition of the essential oil from Stachys serotina. Chem of Natural compounds. 2012;48:508-509.
  27. Goren A.C., et al. Essential oil composition of twenty-two Stachys species (mountain tea) and their biological activities. Phytochemistry Letters. 2011;4:448-453.