Evaluation of Antibacterial, Antibiofilm and Cytotoxicity Effect of Crude Ethanolic Extract of Cuminum cyminum Against Streptococcus mutans

Document Type : Research Paper

Authors

1 Department of Pediatric Dentistry, School of Dentistry, Shahid Beheshti University of Medical Sciences (SBMU) Tehran, Iran

2 Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences (SBMU) Tehran, Iran

3 Faculty of Paramedical Sciences, Mazandaran University of Medical Sciences, Sari, Iran

Abstract

Although, oral health is important for its role in the overall health but oral health care does not have an integrated model. So, today meeting the needs of oral health requires strong interactions between health research policy and oral health researchThe aim of this study investigates the effects of ethanolic crud extract of Cuminum cyminum L. on S. mutans in terms of antibacterial, antibiofilm and, its cytotoxicity properties. The present experimental study which has conducted in 2019-2020. The crude alcoholic extract of C. cyminum seeds was prepared by the maceration method. Then, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of crude ethanolic extract of C. cyminum was determined by micro-dilution method according to CLSI protocol on Streptococcus mutans (PTCC1683). In addition, its antibiofilm effect was investigated by the previous method using crystal violet. As well, an MTT test was performed to evaluate its cytotoxicity on SW480 cells. Statistical analysis was done by SPSS V.22 software. MIC and MBC concentrations of C. cyminum extract on S. mutans were found to be 0.62 mg/mL and 1.25 mg/mL. The effect of inhibiting biofilm production was also observed at a concentration of 40 mg/mL.  In MTT assay showed a greater cytotoxic effect on SW480 cancer cells at higher concentrations for longer periods of time. The present study showed that the cumin ethanolic crude extract has antimicrobial properties, inhibits the growth of S. mutans biofilms and has no toxic properties.
MIC: minimum inhibitory concentration. MBC: minimum bactericidal concentration. MTT: 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyl-2H- tetrazolium bromide. DMSO: dimethyl sulfoxide

Keywords

References

  1. Packiavathy I.A.S.V., Agilandeswari P., Musthafa K.S., Pandian S.K., Ravi A.V. Antibiofilm and quorum sensing inhibitory potential of Cuminum cyminum and its secondary metabolite methyl eugenol against Gram negative bacterial pathogens. Food Res Inter. 2012;45(1):85-92.
  2. 2.https://www.cdc.gov/healthywater/hygiene/disease/dental_caries.html.
  3. Wu J., Fan Y., Wang X., Jiang X., Zou J., Huang R. Effects of the natural compound, xyresveratrol, on the growth of Streptococcus mutans, and on biofilm formation, acid production, and virulence gene expression. European J Oral Sci. 2020;128(1):18-26.
  4. Nassar H.M., Li M., Gregory R.L. Effect of honey on Streptococcus mutans growth and biofilm formation. Applied & Environ Micro. 2012;78(2):536-40.
  5. Mensch K., Nagy G., Nagy Á., Bródy A. Characteristics, diagnosis and treatment of the most common bacterial diseases of the oral cavity. Orvosi hetilap. 2019;160(19):739-46.
  6. Vestby L.K., Grønseth T., Simm R., Nesse L.L. Bacterial biofilm and its role in the pathogenesis of disease. Antibiotics. 2020;9(2):59.
  7. Chen X., Daliri E.B.M., Kim N., Kim J.R., Yoo D., Oh D.H. Microbial etiology and prevention of dental caries: exploiting natural products to inhibit cariogenic biofilms. Pathogens. 2020;9(7):569.
  8. Oliveira B.D.Á., Rodrigues A.C., Cardoso B.M.I., Ramos A.L.C.C., Bertoldi M.C., Taylor J.G., et al. Antioxidant, antimicrobial and anti-quorum sensing activities of Rubus rosaefolius phenolic extract. Industrial Crops & Products. 2016; 84:59-66.
  9. Alejandra B.M., Daniel O.M. Virulence Factors of Streptococcus mutans Related to Dental Caries. Staphylococcus and Streptococcus. 2020:9.
  10. Derakhshan S., Sattari M., Bigdeli M. Effect of subinhibitory concentrations of cumin (Cuminum cyminum L.) seed essential oil and alcoholic extract on the morphology, capsule expression and urease activity of Klebsiella pneumoniae. Inter J Antimicrobial Agents. 2008;32(5):432-6.
  11. Wayne P. A Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing. 30th ed. CLSI supplement M100: Clinical and Laboratory Standards Institute 2020.
  12. O'Tool G., Kaplan H.B., Kolter R. Biofilm formation as microbial development. Annal Rev Microbiol. 2000;54: 49-79.
  13. Kumar G., Jalaluddin M., Rout P., Mohanty R., Dileep C. Emerging trends of herbal care in dentistry. J Clinical & Diagnostic Res: JCDR. 2013;7(8):1827.
  14. Lau D., Plotkin B.J. Antimicrobial and biofilm effects of herbs used in traditional Chinese medicine. Natural Product Communications. 2013;8(11):1934578X1300801129.
  15. Mostafa A.A., Al-Askar A.A., Almaary K.S., Dawoud T.M., Sholkamy E.N., Bakri M.M. Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases. Saudi J Biol Sci. 2018;25(2):361-6.
  16. Lee S-H. Antimicrobial effects of herbal extracts on Streptococcus mutans and normal oral streptococci. J Micro. 2013;51(4):484-9.
  17. Banas JA, Takanami E, Hemsley RM, Villhauer A, Zhu M, Qian F, et al. Evaluating the relationship between acidogenicity and acid tolerance for oral streptococci from children with or without a history of caries. J Oral Micro. 2020;12(1):1688449.
  18. Zhang G., Lu M., Liu R., Tian Y., Vu V.H., Li Y., et al. Inhibition of Streptococcus mutans Biofilm Formation and Virulence by Lactobacillus plantarum K41 Isolated from Traditional Sichuan Pickles. Frontiers in Microbiology. 2020; 11:774.
  19. Sánchez E., Morales C.R., Castillo S., Leos-Rivas C., García-Becerra L., Martínez D.M.O. Antibacterial and antibiofilm activity of methanolic plant extracts against nosocomial microorganisms. Evidence-based Complementary and Alternative Medicine: eCAM. 2016;2016.
  20. Ghazi M., Goudarzi H., Ardekani H.A.Z., Habibi M., Azargashb E., Hajikhani B., et al. Investigation of Antibacterial Effect of Cuminum cyminum and Carum carvi against Streptococcus mutans and Streptococcus pyogenes. Novelty in Biomedicine. 2019;7(1):30-4.
  21. Soleymani N., Sattari M., Sepehriseresht S., Daneshmandi S. Evaluation of reciprocal pharmaceutical effects and antibacterial activity of Bunium persicum essential oil against some Gram positive and Gram negative bacteria. Iranian J Med Microbiol. 2010;4(1):26-34.
  22. Golestannejad Z., Mohammadi E., Motamedi A., Gavanji S., Fallah N., Bagherie S., et al. Chemical composition and antibacterial activity of some herbal essential oils against Streptococcus mutans. Advanced Herbal Medicine. 2016;2(1):1-8.
  23. Ertürk Ö. Antibacterial and antifungal activity of ethanolic extracts from eleven spice plants. Biologia. 2006;61(3):275-8.
  24. Vignesh S., Anitha R., Kumar S.R., Lakshmi T. Evaluation of the antimicrobial activity of cumin oil mediated silver nanoparticles on oral microbes. Res J Pharmacy & Tech. 2019;12(8):3709-12.
  25. Motamedifar, M., Hashemizadeh, Z., Haghighati, G., Yousefi-Avarvand, A., Sedigh, H. Evaluation of Antibacterial Effects of Aqueous and Alcoholic Extracts of Cuminum cyminum L. Seeds on Some Pathogenic Bacteria. Sadra Medical J. 2014; 3(1): 21-30.
  26. Moradi B., Mashak Z., Akhondzadeh Basti A., Moradi B., Barin A. The Survey of the Effect of Cuminum cyminum L. Essential Oil on the Growth of Bacillus cereus in a Food Model System. J Med Plants. 2012;11(41):93-102
  27. Derakhshan S., Sattari M., Bigdeli M. Effect of cumin (Cuminum cyminum) seed essential oil on biofilm formation and plasmid Integrity of Klebsiella pneumoniae. Pharmacogn Mag. 2010 Jan;6(21):57-61.
  28. Arkut A., Özdenefe M.S., Dincer S. Bacterial biofilm.2020. IntechOpen publisher. ISBN: 9781789858990, 1789858992.
Journal of Medicinal plants and By-product
Volume 12, Issue 2
June 2023
Pages 191-196

Files

Share

How to cite

Statistics