Effect of Different Treatments on Dormancy Breaking for Seed Germination Enhancement and Metabolite Analysis of Capparis spinosa L.

Document Type : Research Paper

Authors

1 Agricultural Biotechnology, University Campus 2, University of Guilan, Rasht, Iran

2 Molecular Genetics, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

Abstract

Caper (Capparis spinosa L.) is a Mediterranean multifunctional crop recognized for its drought resistance as well as its capacity to preserve soil moisture. Caper is important in the world due to the important medicinal and nutritional compounds in its roots and leaves and has been considered by the pharmaceutical and food industries. Seed germination is poor owing to physical and physiological dormancy, which makes large-scale cultivation difficult. This research was done on February 2020 in the department of Agriculture, IROST. The effects of gibberellic acid (GA3) at three concentrations of 200, 400, and 600 mg/L, as well as pretreatment with concentrated sulfuric acid (H2SO4 98%) at four-time levels of 15, 30, 45, and 60 minutes, on dormancy breaking in capers, were investigated. Also, total flavonoids, total polyphenols, DPPH assay, and rutin content were analyzed. The findings revealed that 45 minutes of H2SO4 followed by 400 mg/L GA3 was the most efficient method for breaking the dormancy of dried caper seeds, with 81.5% germination, a 3.22 germination rate, and a 10.48 germination index. The highest concentration of metabolic properties in caper leaves was related to total polyphenols (2012.7 mg/g DW). On the other hand, the lowest value was related to rutin content (0.41 mg/g DW). The total flavonoid content was 64.28 mg/g DW, and the DPPH content was 69.06%. So, the results of this study can help improve and grow the output of capers because of the importance of growing them in dry conditions because of their deep root system.

Keywords

References

  1.  

    1. Saghafi Khadem F. Flora of Iran, No. 30: Capparaceae. Published by Research Institute of Forests and Rangelands.1999, 61p.
    2. Jin U.H., Lee J.Y., Kang S.K., Kim J.K., Park W.H., Kim J.G., et al. A phenolic compound, 5-caffeoylquinic acid (chlorogenic acid), is a new type and strong matrix metalloproteinase-9 inhibitor: isolation and identification from methanol extract of Euonymus alatus. Life Sci. 2005;77(22):2760–2769.
    3. Legua P. et al. Phenological growth stages of caper plant (Capparis spinosa) according to the Biologische Bundesanstalt, Bundessortenamt and CHemical scale. Ann. Appl. Biol. 2013; v. 163: n. 1, p. 135-141.
    4. Suleiman M.K., Bhat N.R., Abdal M.S., Jacob S., Thomas R., Al-Dossery S., Bellen R. Germination studies of Capparis spinosa Propag. Ornam. Plants. 2009;9(1):35-38.
    5. Hamilton D.F., Hamilton D.F., Carpenter P.L. Regulation of seed dormancy in Cercis canadensis 1975.
    6. Sozzi G., Chiesa A. Improvement of caper (Capparis spinosa) seed germination by breaking seed coat-induced dormancy. Sci. Hortic. 1995; 62:255-261.
    7. Barbera G., Di Lorenzo R. The caper culture in Italy. In IV International Symposium on Spice and Medicinal Plants. 1983; 144:pp. 167-172.
    8. Bond R.E. The caper bush. The herbarist. 1990; 56:77-85.
    9. Foschi M.L., Juan M., Pascual B., Pascual-Seva N. Water Uptake and Germination of Caper (Capparis spinosa) Seeds. Agronomy. 2020;10(6): 838.
    10. Imani A.F., Sardoei A.S., Shahdadneghad M. Effect of H2SO4 on Seed Germination and Viability of Canna indica Int. j. adv. biol. 2014;2(1):223-229.
    11. Demiral T., Turkan I. Comparative lipid peroxidant, antioxidant systems and praline content in roots of two rice cultivars differing in salt tolerance. Environ. Exp. Bot. 2005; 53:247-257.
    12. Soyler D., Khawar K.M. Seed germination of caper (Capparis ovata var Herbacea) using a naphthalene acetic acid and gibberellic acid. Int J. Agric. Biol. 2007;9 (1):35-8.
    13. Caglar G., Caglar S., Ergin O., Yarim M. The influence of growth regulators on shoot proliferation and rooting of in vitro propagated caper. J. Environ. Biol. 2005;26(3):479-485.
    14. Perry D.A. Methodology and application of vigour tests. International Seed Testing Association, Zurich, Switzerland. 1991; 275pp.
    15. Ashraf M. Foolad M.R. Pre‐sowing seed treatment—A shotgun approach to improve germination, plant growth, and crop yield under saline and non‐saline conditions. Advances in agronomy. 2005; 88:.223-271.
    16. Maguire J.D., Canode C.L. Germination of Latar and Pennlate orchardgrass. In Proceedings of the Association of Official Seed Analysts. Association of Official Seed Analysts. 1963; Vol. 53: pp. 92-95.
    17. Chang C.C., Yang M.H., Wen H.M., Chern J.C. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. food Drug Anal. 2002; 10(3):178-182.
    18. Meda A., Lamien C.E., Romito M., Millogo J., Nacoulma O.G. Determination of the total phenolic, flavonoid and pralin contents in Burkina Fasan honey, as well as their scavenging activity. Food Chem. 2005; 91: 571-577.
    19. Miliauskas G., Venskutonis P.R., Vanbeek T.A. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem. 2004; 85: 231-237.
    20. Xiao J.B., Chen X.Q. RP-HPLC-DAD determination of quercetin, luteolin and apigenin in Marchantia covoluta. Nigerian J. Nat. Pro. Med. 2005; 9: 73-77.
    21. Krauze-Baranowska M., Głód D., Kula M., Majdan M., Hałasa R., Matkowski A., Kozłowska W., Kawiak A. Chemical composition and biological activity of Rubus idaeus shoots a traditional herbal remedy of Eastern Europe. BMC Complement. Altern. Med. 2014; 14:480-452.
    22. Ramezani-Gask M., Bahrani M.J., Shekafandeh A., Salehi H., Taghvaei M. Al-Ahmadi MJ. A comparison of different propagation methods of common Caper-bush (Capparis spinosa) as a new horticultural crop. Int J Plant Dev Biol. 2008; 2:106-110.
    23. Gharoobi B., Ghorbani M., Ghasemi N.M. Effects of different levels of osmotic potential on germination percentage and germination rate of barley, corn and canola. Iran. J. Plant Physiol. 2012;2(2):412-417.
    24. Ahadzadeh B., Zaeifizadeh M., Shahbazi H., Ghasemi M. Effect of terminal drought stress on the germination and growth potential wheat cultivars seeds. Int. J. Biosci. 2014;6655:.134-146.
    25. Azizi H, Rezvani Moghaddam P., Parsa M., Shoor M., Khorasani R. Study on seed dormancy breakage treatments and some germination properties of Colchicum kotschyi as a medicinal plant. Iran. J. Seed Res. 2019;6(3):399-410.
    26. Makizadeh Tafti M., Farhoudi R., Rastifar M., Sadat Asilan K. Seed dormancy failure methods in Capparis spinosa Iranian J. Range and Desert Res. 2011;18(4):569-577.
    27. Hashemi Dezfuli S.A., Agha Alikhani M. Dormancy and Seed Growth, Ahvaz Chamran University Press. 1999;246 p.
    28. Nasiri, M., Madah Arefi, H., Isvand, H.R. Evaluation of viabilitt changes and dormancy breaking in the seed of some species in Natural Resources Gene Bank. IJFPBGR. 2004;12: 163-182.
    29. Zhu G., An L., Jiao X., Chen X., Zhou G., McLaughlin N. Effects of gibberellic acid on water uptake and germination of sweet sorghum seeds under salinity stress. Chil. J. Agric. Res. 2019;79(3):415-424.
    30. Zhang K., Yao L., Zhang Y., Baskin J.M., Baskin C.C., Xiong Z., Tao J. A review of the seed biology of Paeonia species (Paeoniaceae), with particular reference to dormancy and germination. Planta. 2019;249(2):291-303.
    31. Olmez Z., Yahyaoglu Z., Ucler A.O. Effect of H2SO4, GA3 and KNO3 treatment on germination of caper (Capparis ovata) seeds. Pak. J. Biol. Sci. 2004;7(6): 879-882.
    32. Rostamipour A., Moradi A., Isvand H., Nasiri M. Investigation of seed dormancy type and its breaking methods in 3 ecotypes Iranian Astragale (Astragalus cyclophyllus) Pasture Plant. Iran. J. Sci. Technol. Seed. 2015; 4:51-56.
    33. Nowruzian A., Masoumian M., Ebrahimi A., Bakhshi Khaniki GH. Effect of Breaking Dormancy Treatments on Germination of Ferula assa foetida Seed. Iran. J. Seed Res. 2017;3(2):154-169. (In Persian)
    34. Orphanos P.L. Germination of Caper (Capparis ovata) seeds. J. Hort, Sci. 1983;58(2):267-270.
    35. Labbafi M.R., Mehrafarin A., Badi H.N., Ghorbani M., Tavakoli M. Improve germination of caper (Capparis spinosa) seeds by different induction treatments of seed dormancy breaking. Trakia J. Sci. 2018;16(1):71-74.
    36. Fang L., Qingfeng L., shujun L., Jun X. Some methods for stimulating germination of carex Pratacultural Science. 1998;15(5):39-48.
    37. Aliero B.L. Effects of sulphuric acid, mechanical scarification and wet heat treatments on germination of seeds of Parkia biolobosa. j. biotechnol. 2004; 3:179-181.
    38. Vaisi GH., Mohtadi A., Moradi A. The effect of different treatments on seed germination and dormancy breaking in seeds of Gundelia tournefortii- Nova Biologica Rep. 2018; 5:26-37.
    39. Heydariyan M., Basirani N., Sharifi-Rad M., Khmmari I., Poor S.R. Effect of seed priming on germination and seedling growth of the caper (Capparis Spinosa) under drought stress. Int. j. adv. biol. 2014;2(8):2381-2389.
    40. Vahid H, Rakhshandeh H, Ghorbani A. Antidiabetic properties of Capparis spinosa and its components. Biomed. Pharmacother. 2017; 92, 293–302.
    41. Zhang H., Ma Z.F. Phytochemical and Pharmacological Properties of Capparis spinosa as a Medicinal Plant. Nutrients. 2018; 10(116):1-14.
    42. Mollica A., Zengin, G., Locatelli M., Stefanucci A., Mocan A., Macedonio G., Carradori S., Onaolapo O., Onaolapo A., Adegoke J., et al. Anti-diabetic and anti-hyperlipidemic properties of Capparis spinosa: In vivo and in vitro evaluation of its nutraceutical potential. J. Funct. Foods. 2017; 35, 32–42.
    43. AL-Azawi A.H., Ghaima K.K., Salih H.H. Phytochemical, antibacterial and antioxidant activities of Capparis spinosa cultivated in Iraq. Biosci. Res. 2018; 15(3):2611-2618.
    44. Tlili N., Saadaoui E., Sakouhi F., Elfalleh W., El Gazzah M., Triki1 S., Abdelhamid Kh. Morphology and chemical composition of Tunisian caper seeds: variability and population profiling. Université Tunis El-Manar, Tunis, Afr. J. Biotechnol. 2011; 10 (10):2112-2118.
    45. Yu L, Yang J, Wang X, Jiang B, Sun Y, Ji Y. Antioxidant and antitumor activities of Capparis spinosa L. and the related mechanisms. Oncol. Rep. 2017; 37(1): 357-367.
    46. Aliyazicioglu R., Eyupoglu O.E., Huseyin S., Oktay Y., Nimet B. Phenolic components, antioxidant activity, and mineral analysis of Capparis spinosa Afr. J. Biotechnol. 2013; 12(47):6643-6649.
    47. Mansour R.B., Jilani I.B.H., Bouaziz M, Gargouri B., Elloumi N., Attia H., Ghrabi-Gammar Z., Lassoued S. Phenolic contents and antioxidant activity of ethanolic extract of Capparis spinosa. Cytotechnology. 2016; 68(1):135-142.
Journal of Medicinal plants and By-Products
Volume 12, Issue 4
December 2023
Pages 387-396

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