Secondary Metabolites and Antioxidant Activity in Different Iranian Accessions of Dorema ammoniacum D. Don and Dorema aucheri Boiss.

Document Type : Research Paper

Authors

1 Department of Natural Resources, Isfahan University of Technology, Isfahan 84156-83111, Iran

2 Research Institute for Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan 84156-83111, Iran

3 Department of Plant and Animal Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran

Abstract

This study was performed to evaluate different secondary metabolites (SMs) and antioxidant activity in 14 different genotypes from methanolic extracts of plant samples (leaves and stem) from Dorema ammoniacum D. Don (D.am) and D. aucheri Boiss. (D.au). The contents of SMs and antioxidant activity were measured using UV-visible spectrophotometer. The highest total phenolics content (34.3 mg/GAE DW) and antioxidant activity (92%) through DPPH method were detected in leaves samples of D.au9 genotype. Highest levels of total flavonoids (5.5 mg QE/g DW) and total flavonols (3.6 mg QE/g DW) were observed in leaves of D.au12 and D.au2, respectively. The highest content of anthocyanin (250 nano mol/g FW) and carotenoids (18 mg/g DW) were found in leaves samples of D.au7 and D.au3 genotypes. The results showed the superiority of D. aucheri for different SMs rather than D. ammoniacum Finally, leaves samples of D. aucheri and D. ammoniacum species showed higher contents for secondary metabolites which may be suggested for further appliances with medicinal and industrial aims.

Keywords

Main Subjects

References

  1. Erb M., Kliebenstein D.J. Plant secondary metabolites as defenses, regulators, and primary metabolites: the blurred functional trichotomy. Americ Soc Plant Biologists. 2020; 184: 39-52.
  2. Khare S., Singh N.B., Singh A., Hussain I., Niharika K.M., Yadav V., Bano C., Yadav R.K., Amist N. Plant secondary metabolites synthesis and their regulations under biotic and abiotic constraints. J Plant Biol. 2020; 63: 203-216.
  3. Xu D.P., Li Y., Meng X., Zhou T., Zhou Y., Zheng J., Zhang J.J., Li HB. Natural antioxidants in foods and medicinal plants: extraction, assessment and resources. Int J Mol Sci. 2017; 18: 96.
  4. Raut JS, Karuppayil S.M. A status review on the medicinal properties of essential oils. Ind Crops Prod. 2014; 62: 250-264.
  5. Li Y., Kong D., Fu Y., Sussman M.R., Wu H. The effect of developmental and environmental factors on secondary metabolites in medicinal plants. Plant Physiol Biochem. 2020; 148: 80-89.
  6. Yang L., Wen K.S., Ruan X., Zhao Y.X., Wei F., Wang Q. Response of plant secondary metabolites to environmental factors. Molecules 2018, 23: 762
  7. Belkheir A.K., Gaid M., Liu B., Hänsch R., Beerhues L. Benzophenone synthase and chalcone synthase accumulate in the Mesophyll of Hypericum perforatum Leaves at different developmental stages. Front in Plant Sci. 2016; 7: 921.
  8. Zibaee E., Amiri M. S., Boghrati Z., Farhadi F., Ramezani M., Emami S.A., Sahebkar A.H. Ethnomedicinal Uses, Phytochemistry and Pharmacology of Dorema Species (Apiaceae): A Review. J Pharmacopuncture. 2020; 23: 91-123.
  9. Mozaffarian V. Flora of Iran. No. 54: Umbelliferae. Publications of Forests and Rangelands Research Institute. 1st ed, Tehran. 2007.
  10. Eftekhari M., Oskou F., Tofighi Z., Motevaseli E., Jafari S., Nodooshan S., Shams-Ardekani MR. Alert for Consumption of Dorema aucheri: an Edible Medicinal Plant of Iran. J Med Plants. 2019; 18:77-84.
  11. Khanahmadi M., Miraghaee SSh., Karimi I. Evaluation of the Antioxidant and antimicrobial Properties of Dorema aucheri plant. Iranian Red Crescent Med J. 2012, 14: 684-685.
  12. Iravani N., Solouki M., Omidi M., Saidi A., Zare A. Seed germination and dormancy beaking in Dorema ammoniacum an endangered medicinal plant. Trakia J Sci. 2012; 10: 9-15.
  13. Batooli H., Haghir-Ebrahimabadi A.R., Mahmoudi B., Mazuchi A. Comparison of the chemical composition of essential oils of vegetative and reproductive organs of Dorema ammoniacum D Don. in Shahsavaran region of Kashan. Iranian J Med Arom Plants. 2014; 5: 746-755.
  14. Zandpour F., Vahabi M.R., Allafchian A.R., Farhang H.R. Phytochemical investigation of the essential oils from the leaf and stem of Dorema ammoniacum D. Don. (Apiaceae) in Central Zagros, Iran J Herb Drug. 2016; 7: 109-116.
  15. Masoudi S., Kakavand S. Volatile constituents of the aerial parts of Terataenium lasiopentalum (Boiss.) Manden., stems and leaves of Dorema ammoniacum D. Don. and leaves, fruits and stems of Leutea petiolare (DC.) M. Pimen from Iran. J Chilean Chem Soc. 2017; 62: 3311-3314.
  16. Mianabadi M., Hoshani M., Salmanian S. Antimicrobial and antioxidative effects of methanolic extract of Dorema aucheri Boiss. J Agric Sci Technol. 2015; 17: 623-634.
  17. Akbarian A., Rahimmalek M., Sabzalian M.R., Saeedi Q. Evaluation of phytochemical, morphological and antioxidant activity of Dorema aucheri populations cultivated in different environments. J Med Plants. 2017; 16: 120-135.
  18. Singleton V.L., Orthofer R., Lamuela-Raventós R.M. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol. 1999; 299: 152-178.
  19. Chang C., Yang M., Wen H., Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 2002; 10: 178-182.
  20. Golkar P., Taghizadeh M., Jalali SAH. Determination of phenolic compounds, antioxidant and anticancer activity of Chrozophora tinctoria accessions collected from different regions of Iran. J Food Biochem. 2019; 43: 1-14.
  21. Lichtenthaler H., Wellburn A. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Transactions. 1983; 11: 591-592.
  22. Ahmadi H., Morshedloo M.R., Emrahi R., Javanmard A., Rasouli F., Maggi F., Kumar M., Lorenzo J.M. Introducing three new fruit-scented mints to farmlands: insights on drug yield, essential-oil quality, and antioxidant properties. Antioxidants. 2022; 11(5), 866.
  23. Team RC. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Aust. 2017.
  24. Ndhlala A.R., Moyo M., Van-Staden J. Natural antioxidants: fascinating or mythical biomolecules? Molecules. 2010; 15: 6905-6930.
  25. Pollastri S., Tattini M. Flavonols: old compounds for old roles. Ann Bot. 2011, 108: 1225-1233.
  26. Pandey G, Khatoon S, Pandey MM, Rawat AKS. Altitudinal variation of berberine, total phenolics and flavonoid content in Thalictrum foliolosum and their correlation with antimicrobial and antioxidant activities. J Ayurveda Integr Med. 2018; 9: 169-176.
  27. Zargoosh Z., Ghavam M., Bacchetta G., Tavili A. Effects of ecological factors on the antioxidant potential and total phenol content of Scrophularia striata Boiss. Sci Rep. 2019; 9: 1-15.
  28. Chrysargyris A, Mikallou M, Petropoulos S, Tzortzakis N. Profiling of essential oils components and polyphenols for their antioxidant activity of medicinal and aromatic plants grown in different environmental conditions. Agron. 2020, 10: 727.
  29. Gairola S., Sharif N., Bhate A., Prakashkala C. Influence of climate change on production of secondary chemicals in high altitude medicinal plants. J Med Plants Res. 2010; 4: 1825-1829.
  30. Saboora A., Ahmadi A., Zeinali A., Parsa M. Comparison of the content of phenolic compounds, favonoids and antioxidant activity of the two extremities in northwestern Iran. J Rafsanjan Univ Med Sci. 2013; 13: 249-266.
  31. Norouzi V., Yousefzadeh S., Asilan K., Mansoorifar S. Investigating the variation of essential oil content, chlorophyll, carotenoid, anthocyanin and flavonoid of Mentha Longifolia (L.) Hods. subsp. Longifolia in several habitats of Marand. Eco-Phytochemical J Med Plants. 2017; 17: 52-64.
  32. Zhang Y., Butelli E., Martin C. Engineering anthocyanin biosynthesis in plants. Curr Opin Plant Biol. 2014, 19: 81-90.
Journal of Medicinal plants and By-product
Volume 12, Issue 4
December 2023
Pages 459-468

Files

Share

How to cite

Statistics