Capparis spinosa L. Reduces Cisplatin-induced Acute Liver Injury in Male Rats: Pretreatment and Single Dose Therapy

Document Type : Research Paper

Authors

1 Department of Persian Medicine, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran

2 Department of Pharmacology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran

3 Department of Public Health, School of Public Health, Zanjan University of Medical Sciences, Zanjan, Iran

4 Metabolic Diseases Research Center, Zanjan University of Medical Sciences, Zanjan, Iran

Abstract

Cisplatin as a chemotherapeutic agent causes liver injury by increasing inflammatory production. Capparis spinosa L. as a source of natural antioxidants can clear this production. The present study was designed to assess the effects of pretreatment as well as treatment with a single dose of hydroalcoholic extract of Capparis seed on cisplatin-induced liver damage in rats. Forty-eight male Sprague-Dawley rats were divided into six groups (control group, Cis (cisplatin) group, 200 C/S (C. spinosa), Cis + 50 C/S 1-day, Cis + 100 C/S 1-day, and 100 C/S + Cis groups). Biochemical and histopathological assessment were done. Statistical analyses were performed with Graph Pad Prism Statistics software 9.1.2. The level of significance was set at p < 0.05. Liver function tests, antioxidant and inflammatory parameters and quantitative parameters of histopathological changes were measured. Significant changes in the pathology results were obvious. The diameter of central vein, portal vein, and bile duct, the thickness of the hepatic artery wall, and hepatic sinusoids were significantly increased in the Cis and 200 C/S-fed groups, compared to the controls, and also changes in favor of improvement were evident in the treatment groups compared to the Cis and 200 C/S groups. By increasing the time interval between cisplatin injection and testing, as well as using the western blotting method to measure the level of antioxidant and inflammatory markers, we may have significant biochemical and antioxidant results. Based on pathology results, single-dose treatment with C. spinosa seed extract may be beneficial in the cisplatin-induced liver damage.

Keywords

References

  1. Anushiravani A., Ghajarieh Sepanlou S. Burden of Liver Diseases: A Review from Iran. Middle East J Dig Dis. 2019;11(4):189-191.
  2. Marcellin P., Kutala B.K. Liver diseases: A major, neglected global public health problem requiring urgent actions and large-scale screening. Liver Int. 2018;38 Suppl 1(S1):2-6.
  3. Asrani S.K., Devarbhavi H., Eaton J., Kamath PS. Burden of liver diseases in the world. J Hepatol. 2019;70(1):151-171.
  4. Omar H.A., Mohamed W.R., Arab HH, Arafa el S.A. Tangeretin Alleviates Cisplatin-Induced Acute Hepatic Injury in Rats: Targeting MAPKs and Apoptosis. PLoS One. 2016;11(3): e0151649.
  5. Omar H.A., Mohamed W.R., Arafa el S.A., Shehata B.A., El Sherbiny G.A., Arab H.H., Elgendy A.N. Hesperidin alleviates cisplatin-induced hepatotoxicity in rats without inhibiting its antitumor activity. Pharmacol Rep. 2016;68(2):349-356.
  6. Hosseini S.M.R., Ghayour Razmgah G.R., Nematy M., Esmaily H., Yousefi M., Kamalinejad M., Mosavat SH. Efficacy of Black Seed (Nigella sativa) and Lemon Balm (Melissa officinalis) on Non-Alcoholic Fatty Liver Disease: A Randomized Controlled Clinical Trial. Iranian Red Crescent Medical J. 2018;20(3).
  7. Ibrahim L., El-Ansari M., Sharaf M. Capparis spinosa L.: a natural source of pharmaceuticals. Egyptian Pharmaceutical J. 2018;17(2):61.
  8. Sardari S., Fallahiarezoudar F., Emadi F., Davati A., Faramarzi E., Fesharaki M.G., Esmaeili S.S. CAPPARIS A MULTIFUNCTIONAL HERB: EVIDENCE FROM THE IRANIAN TRADITIONAL MEDICINE TO MODERN MEDICINE. Indo American J Pharmaceutical Sci. 2017;4(12).
  9. Manikandaselvi S., Vadivel V., Brindha P. Review on ethnobotanical studies of nutraceutical plant: Capparis spinosa L.(Caper). Asian J Pharm Clin Res. 2016;9:123-126.
  10. Moufid A., Farid O., Eddouks M. Pharmacological Properties of Capparis spinosa Linn. International J Diabetology & Vascular Disease Res. 2015;3(5):99-104.
  11. Allaith AAA. Assessment of the antioxidant properties of the caper fruit (Capparis spinosa L.) from Bahrain. J the Association of Arab Universities for Basic and Applied Sciences. 2018;19(1):1-7.
  12. Ennacerie F.Z., Rhazi Filali F., Moukrad N., Bouidra M., Bentayeb A. Evaluation of the Antioxidant Activity and the Cytotoxicity of Extracts of Capparis spinosa. International J Pharmaceutical Sci and Drug Res. 2018;10(02):57-64.
  13. Khavasi N., Somi M., Khadem E., Ayati M.H., Torbati M., Fazljou S.M.B. Daily Consumption of the Capparis spinosa Reduces Some Atherogenic Indices in Patients with Non-alcoholic Fatty Liver Disease: A Randomized, Double-blind, Clinical Trial. Iranian Red Crescent Medical J. 2018;In Press(In Press).
  14. Ghassemifard L., Sardari S., Safari H., Ramezanikhah H., Khavasi N. Assessment of the antioxidant activity of the extrcat, cold press, and n-hexane oils and in vitro cytotoxic effects of Capparis spinosa L. seed on SH-SY5Y cancer cell lines. J Medicinal Plants. 2022;21(83):87-98.
  15. El-Sharouny SH., Rizk AAEE, Rashed L.A., Sayed W.M., Elmoneam M. Analysis of the therapeutic role of platelet-rich plasma against cisplatin-induced hepatotoxicity in rats: controversy between oxidative and apoptotic markers. Eur J Anat. 2019;23(3):201-213.
  16. Tir M., Feriani A., Labidi A., Mufti A., Saadaoui E., Nasri N., Khaldi A., El Cafsi M., Tlili N. Protective effects of phytochemicals of Capparis spinosa seeds with cisplatin and CCl4 toxicity in mice. Food Bioscience. 2019;28:42-48.
  17. Fanoudi S., Rakhshandeh H., Afshari A.R., Mollazadeh H., Taher M. Nephrotoxicity and Hepatotoxicity of Capparis Spinosahydro-Alcoholic Extract in Mice. JOJ Urology & Nephrology. 2017;4(2):555640.
  18. Kalantari H., Foruozandeh H., Khodayar M.J., Siahpoosh A., Saki N., Kheradmand P. Antioxidant and hepatoprotective effects of Capparis spinosa L. fractions and Quercetin on tert-butyl hydroperoxide- induced acute liver damage in mice. J Tradit Complement Med. 2018;8(1):120-127.
  19. Mahmoodpoor F., Hosseini S., Ahmadian E., Ardalan M., Kamali K., Sardari S., Khavasi N. Hydroalcoholic extract of Capparis spinosa seeds reduces cisplatin-induced nephrotoxicity in rats. Eurasian Chemical Communications. 2022;4(3):263-271.
  20. Ali M., Khan T., Fatima K., Ali Q.U.A., Ovais M., Khalil A.T., Ullah I., Raza A., Shinwari Z.K., Idrees M. Selected hepatoprotective herbal medicines: Evidence from ethnomedicinal applications, animal models, and possible mechanism of actions. Phytother Res. 2018;32(2):199-215.
  21. Tlili N, Feriani A., Saadoui E., Nasri N., Khaldi A. Capparis spinosa leaves extract: Source of bioantioxidants with nephroprotective and hepatoprotective effects. Biomed Pharmacother. 2017;87:171-179.
  22. Wojdylo A., Nowicka P., Grimalt M., Legua P., Almansa M.S., Amoros A., Carbonell-Barrachina AA, Hernandez F. Polyphenol Compounds and Biological Activity of Caper (Capparis spinosa L.) Flowers Buds. Plants (Basel). 2019;8(12):539.
  23. Gull T., Anwar F., Sultana B., Alcayde M.A.C., Nouman W. Capparis species: A potential source of bioactives and high-value components: A review. Industrial Crops and Products. 2015;67:81-96.
  24. Bhoyar M.S., Mishra G.P., Naik P.K., Singh S.B. Evaluation of Antioxidant Capacities and Total Polyphenols in Various Edible Parts of Capparis spinosa L. Collected from Trans-Himalayas. Defence Life Sci J. 2018;3(2):30-36.
  25. Aichour R., Benzidane N., Arrar L., Charef N., Baghiani A. Hepatoprotective and Anti-inflammatory Activities of Algerian Capparis spinosa. L. Annual Res & Review in Biology. 2018;25(3):1-12.
  26. Aghel N., Rashidi I., Mombeini A. Hepatoprotective activity of Capparis spinosa root bark against CCl4 induced hepatic damage in mice. Iranian J Pharmaceutical Research. 2007.
  27. Khavasi N., hosein Somi M., Khadem E., Faramarzi E., Ayati M.H., Fazljou S.M.B., Torbati M. Effect of daily caper fruit pickle consumption on disease regression in patients with non-alcoholic fatty liver disease: a double-blinded randomized clinical trial. Advanced pharmaceutical bulletin. 2017;7(4):645.
  28. Mohebali N., Shahzadeh Fazeli S.A., Ghafoori H., Farahmand Z., MohammadKhani E., Vakhshiteh F., Ghamarian A, Farhangniya M., Sanati M.H. Effect of flavonoids rich extract of Capparis spinosa on inflammatory involved genes in amyloid-beta peptide injected rat model of Alzheimer's disease. Nutr Neurosci. 2018;21(2):143-150.
  29. Eddouks M., Lemhadri A., Hebi M., El Hidani A., Zeggwagh N.A., El Bouhali B., Hajji L., Burcelin R. Capparis spinosa L. aqueous extract evokes antidiabetic effect in streptozotocin-induced diabetic mice. Avicenna J Phytomed. 2017;7(2):191-198.
  30. Rahmani R., Mahmoodi M., Karimi M., Hoseini F., Heydari R., Salehi M., Yousefi A. Effect of hydroalcoholic extract of Capparis spinosa fruit on blood sugar and lipid profile of diabetic and normal rats. Zahedan J Res in Medical Sciences. 2013;15(11):34-38.
  31. Tajik M., Seifi S., Feizi F., Kazemi S., Moghadamnia A. Histopathological evaluation of hydroalcoholic extraction of capparisspinosa on the oral wound healing in rats. Journal of Babol University of Medical Sci. 2016;18(12):33-39.
Journal of Medicinal plants and By-Products
Volume 12, Issue 4
December 2023
Pages 513-524

Files

Share

How to cite

Statistics