Investigating the Storage Conditions of the Essential Oil Compounds of Garden Thyme

Document Type : Research Paper

Authors

1 Department of Horticultural Science, Yasuj Branch, Islamic Azad University, Yasuj, Iran

2 Department of Agriculture, Payame Noor University, Tehran, Iran

3 Sustainable Agriculture and Food Security Research Group, Yasuj Branch, Islamic Azad University, Yasuj, Iran

Abstract

The changes in the compositions of essential oils (EOs) from the aerial parts of Thymus vulgaris (garden thyme) were determined at different temperatures and storage times. In this study, the effect of time and temperature on the quality of essential oils was investigated. The essential oil of air-dried samples was obtained by hydro-distillation and was analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). Changes in essential oil compositions were detected during storage for three months in a refrigerator (4 ◦C), a freezer (−20 ◦C), and at room temperature. In thyme, the amount of the important thymol compound was stable until the first two months, but when entering the third month, a significant decrease was observed in all three temperature conditions. At the same time, a significant increase was observed in the amount of p-cymene in each of the temperature conditions. In the end, we can introduce the best temperature maintenance conditions for thyme at room temperature that can have a lower cost from an economic point of view than other conditions.

Keywords


  1. Flores Y., Pelegrín C.J., Ramos M., Jiménez A., Garrigos M.C. Use of herbs and their bioactive compounds in active food packaging. Aromatic Herbs in Food. 2021;323-365.
  2. Contrada M., Cerasa A., Tonin P., Bagetta G., Scuteri D. Aromatherapy in Stroke Patients: Is it Time to Begin? Frontiers in Behavioral Neuroscience. 2021;15:749353.
  3. Zhang Y., Long Y., YuaDanLi SH., Yang M, Guan O., Zhang D., Wana J., Liu S NanLia SH., Penga W. Natural volatile oils derived from herbal medicines: A promising therapy way for treating depressive disorder. Pharmacological Res. 2021;164:105376
  4. Semnani S.N., Hajizadeh N., Alizadeh H. Antibacterial effects of aqueous and organic quince leaf extracts on gram–positive and gram–negative bacteria. Banats J Biotechnology. 2017;16:57.
  5. Sanjida S.J., Fahmee S., Mitu Sh.,  Shahriar M.,  Bhuiyan M.A. Genomic DNA extraction methods: A comparative case study with gram-negative organisms. 2015;11:61.
  6. Zare Bidaki M., Arab M., Khazaei M., Afkar E., Zardast M. Anti-Bacterial Effect of Zataria multiflora Boiss. Essential Oil on Eight Gastrointestinal Pathogenic Species. Quarterly of Horizon of Medical Sci. 2015;21:155-161.
  7. Eftekhar F., Zamani S., Yusefzadi M., Hadian J., Ebrahimi S.N. Antibacterial activity of Zataria multiflora Boiss essential oil against extended spectrum β lactamase produced by urinary isolates of Klebsiella pneumonia. Jundishapur J Microbiol. 2011;4:1.
  8. Saei-Dehkordi S.S., Tajik H., Moradi M., Khalighi-Sigaroodi F. Chemical composition of essential oils in Zataria multiflora Boiss. from different parts of Iran and their radical scavenging and antimicrobial activity. Food and Chemical Toxicology. 2010;48:1562-1567.
  9. Tiwari B.K., Valdramidis V.P., Donnell C.P.O., Muthukumarappan K., Bourke P., Cullen P.J. Application of natural antimicrobials for food preservation. J Agric and Food Chem. 2009;57:5987-6000.
  10. Mansour A., Enayat K., Neda M.S., Behzad A. Antibacterial effect and physicochemical properties of essential oil of Zataria multiflora Boiss. Asian Pacific J Tropical Medicine. 2010;3:439-442.
  11. Adams R.P. Identification of essential oils by gas chromatography quadrupole mass spectrometry. Allured Publishing Corporation, Carol Stream, USA. 2001.
  12. Escobar A., Pérez M., Romanelli G., Blustein G. Thymol bioactivity: A review focusing on practical applications. Arabian J Chem. 2020;13:9243-9269.
  13. Rowshan V., Bahmanzadegan A. Saharkhiz M.J. Influence of storage conditions on the essential oil composition of Thymus daenensis Celak. Industrial Crops and Products. 2013;49:97-101.
  14. Kumar R., Sharma S., Sood S., Agnihotri V.K., Singh B. Effect of diurnal variability and storage conditions on essential oil content and quality of damask rose (Rosa damascena Mill.) flowers in north western Himalayas. Scientia Horticulturae. 2013;154:102-108.
  15. Najafian SH. Storage conditions affect the essential oil composition of cultivated Balm Mint Herb (Lamiaceae) in Iran. Industrial Crops and Products. 2014;52:575-581.
  16. Afshar M., Najafian SH., Radi M. Seasonal variation on the major bioactive compounds: total phenolic and flavonoids contents, and antioxidant activity of rosemary from shiraz. Natural Product Res. 2021;1-6.