Effect of Environmental Stress on Physical Properties and Antibacterial Activity of Atlas Cedar (Cedrus atlantica) Oil-in-water Emulsion

Document Type : Research Paper


1 Faculty of Fisheries and Food Science, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

2 Department of Engineering, Tabriz Branch, Islamic Azad University, Tabriz, Iran


Synthetic preservative compounds can prevent pathogenic bacterial growth, but they cause other concerns related to the adverse effect on human health. Essential Oil (EO), which possesses antibacterial activity, have potential replacers for synthetic preservatives. This study was conducted to develop Atlas cedar EO antibacterial activity, physical properties and sustainability against environmental stress via emulsification. Firstly, screening to select the most potent EO among various EOs (i.e. anise, Atlas cedar, curry leaf and onion) was done. As Atlas cedar was the most efficient antibacterial agent, emulsions containing Atlas Cedar EO were subsequently prepared using different concentrations of Polysorbate20 via a solvent-displacement technique. The physical properties (droplets size, stability, lightness and turbidity) and antibacterial activity (agar disk diffusion) of emulsions were determined. Results showed that emulsion containing 7% (wt) of Polysorbate20 was the most desirable sample in terms of physical properties of antibacterial activity. Henceforth, it was selected for environmental stresses study (i.e. thermal processing, freeze-thaw cycle and ultraviolet exposure). Results revealed that all types of environmental stresses had a significant (p<0.05) effects on physical properties. Environmental stress treatments showed antibacterial activity enhancement against Gram-positive bacteria. Thus, the present work proved the potential use of emulsion as the delivery system of EO as antibacterial agent for applications in the food industry.


  1. Karaman R. Commonly Used Drugs-Uses, Side Effects, Bioavailability and Approaches to Improve It: Uses, Side Effects, Bioavailability & Approaches to Improve it. Nova Science Publishers, Incorporated. 2015.
  2. Anand S., Sati N. Research, Artificial preservatives and their harmful effects: looking toward nature for safer alternatives. International J Pharmaceutical Sci. 2013; 4(7): 2496.
  3. Al-Mansoori D.H. Efficacy of essential oil nanoemulsion delivery system for strong antimicrobial action against pathogen Listeria monocytogenes. Rutgers University-School of Graduate Studies. 2018.
  4. Rhafouri R., et al. Chemical composition, antibacterial and antifungal activities of the Cedrus atlantica (Endl.) Manettiex Carrière seeds essential oil. Mediterranean Journal of Chemistry. 2014; 3(5):1034-1043.
  5. Boudarene L., et al. Composition of the seed oils from Algerian Cedrus atlantica G. Manetti. J Essential Oil Res. 2004; 16(1): 61-63.
  6. Derwich E., Benziane Z., Boukir A. Chemical composition and in vitro antibacterial activity of the essential oil of Cedrus atlantica. International J. Agric. Biology. 2010; 12(3): 381-385.
  7. Fernández-López J., Viuda-Martos M. Introduction to the special issue: Application of Essential Oils in Food Systems. Foods. 2018.
  8. Cassini A., et al. Impact of food and water-borne diseases on European population health. Current Opinion in Food Sci. 2016;12: 21-29.
  9. Maté J., Periago P.M., Palop A. Combined effect of a nanoemulsion of d-limonene and nisin on Listeria monocytogenes growth and viability in culture media and foods. Food Sci. Technology International. 2016;22(2): p. 146-152.
  10. McClements D., Emulsion stability. Food emulsions, principles, practices, techniques. 2005; 269-339.
  11. Anarjan N., et al. Effects of pH, ions, and thermal treatments on physical stability of astaxanthin nanodispersions. International Journal of Food Properties. 2014; 17(4): 937-947.
  12. Topuz O.K., et al. Physical and antimicrobial properties of anise oil loaded nanoemulsions on the survival of foodborne pathogens. Food Chemistry. 2016; 203:117-123.
  13. Zhang S., et al. Preparation and characterization of blended cloves/cinnamon essential oil nanoemulsions. LWT-Food Science and Technology. 2017; 75: 316-322.
  14. Ribeiro H.S., et al. Preparation of nanodispersions containing β-carotene by solvent displacement method. Food Hydrocolloids. 2008; 22(1): 12-17.
  15. Komaiko J. Optimization of the fabrication, stability, and performance of food grade nanoemulsions with low and high energy methods. 2016, University of Massachusetts Amherst.
  16. Taghavi E., et al. Effect of microfluidization condition on physicochemical properties and inhibitory activity of nanoemulsion loaded with natural antibacterial mixture. Food and Bioprocess Technology. 2018; 11(3): 645-659.
  17. Taghavi E., et al. Formulation and functionalization of linalool nanoemulsion to boost its antibacterial properties against major foodborne pathogens. Food Bioscience. 2021; 44:101430.
  18. Choo J., Rukayadi Y., Hwang J.K. Inhibition of bacterial quorum sensing by vanilla extract. Letters in Applied Microbiology. 2006; 42(6):637-641.
  19. Galvão K., Vicente A., Sobral P. Development, characterization, and stability of O/W pepper nanoemulsions produced by high-pressure homogenization. Food and Bioprocess Technology. 2018; 11(2):355-367.
  20. Sheng B., et al. Physicochemical properties and chemical stability of β-carotene bilayer emulsion coated with bovine serum albumin and Arabic gum compared to monolayer emulsions. Molecules. 2018; 23(2):495.
  21. Aoki T., Decker E.A., McClements D.J. Influence of environmental stresses on stability of O/W emulsions containing droplets stabilized by multilayered membranes produced by a layer-by-layer electrostatic deposition technique. Food Hydrocolloids. 2005; 19(2):209-220.
  22. Jafari R., Zandi M., Ganjloo A. Effect of ultrasound and microwave pretreatments on extraction of anise (Pimpinella anisum L.) seed essential oil by ohmic-assisted hydrodistillation. Journal of Applied Research on Medicinal and Aromatic Plants. 2022;100418.
  23. Kačániová M., et al. Chemical Composition, Antioxidant, In Vitro and In Situ Antimicrobial, Antibiofilm, and Anti-Insect Activity of Cedar atlantica Essential Oil. Plants. 2022; 11(3):358.
  24. Rajendran M.P., Pallaiyan B.B., Selvaraj N. Chemical composition, antibacterial and antioxidant profile of essential oil from Murraya koenigii (L.) leaves. Avicenna J Phytomedicine. 2014; 4(3):200-14.
  25. Vazquez-Armenta F., Cruz-Valenzuela M., Ayala-Zavala J. Onion (Allium cepa) essential oils, in Essential Oils in Food Preservation Flavor and Safety. 2016; Elsevier. 617-623.
  26. Johansen J.D. Essential oils: General aspects in Monographs in Contact Allergy: Fragrances and Essential Oils (Volume 2) by Anton C. de Groot, Taylor & Francis Group. 2019; LWW. p. 671.
  27. de Cássia Da Silveira e Sá R., Andrade L.N., De Sousa D.P. Sesquiterpenes from essential oils and anti-inflammatory activity. Natural Product Communications. 2015; 10(10):1934578X1501001033.
  28. Hammer K.A., Carson C.F. Antibacterial and antifungal activities of essential oils. Lipids and Essential Oils as Antimicrobial Agents. 2011; 255-306.
  29. Sikkema J., de Bont J.A., Poolman B. Interactions of cyclic hydrocarbons with biological membranes. J Biological Chemistry. 1994; 269(11):8022-8028.
  30. Ultee A., et al. Adaptation of the food-borne pathogen Bacillus cereus to carvacrol. Archives of Microbiology. 2000; 174(4):233-238.
  31. Weber F.J., de Bont J.A. Adaptation mechanisms of microorganisms to the toxic effects of organic solvents on membranes. Biochimica et Biophysica Acta (BBA) -Reviews on Biomembranes. 1996; 1286(3):225-245.
  32. Matsaridou I., et al. The influence of surfactant HLB and oil/surfactant ratio on the formation and properties of self-emulsifying pellets and microemulsion reconstitution. AAPS PharmSciTech. 2012; 13(4):1319-1330.
  33. Kanafusa S., Chu B.S., Nakajima M. Factors affecting droplet size of sodium caseinate‐stabilized O/W emulsions containing β‐carotene. European Journal of Lipid Science and Technology. 2007; 109(10):1038-1041.
  34. Xue J. Essential oil nanoemulsions prepared with natural emulsifiers for improved food safety. 2015; University of Tennessee.
  35. Kabalnov A.S. Coalescence in emulsions, in Modern aspects of emulsion science, B.P. Binks, Editor. The Royal Society of Chemistry. 1998;205-260.
  36. Qian C., McClements D.J. Formation of nanoemulsions stabilized by model food-grade emulsifiers using high-pressure homogenization: Factors affecting particle size. Food Hydrocolloids. 2011; 25(5):1000-1008.
  37. Oyedeji O.A., Afolayan A., Eloff J. Comparative study of the essential oil composition and antimicrobial activity of Leonotis leonurus and L. ocymifolia in the Eastern Cape, South Africa. South African J Botany. 2005; 71(1):114-116.
  38. Liang W., Lam J.K. Endosomal escape pathways for non-viral nucleic acid delivery systems, in Molecular Regulation of Endocytosis, B. Ceresa, Editor. 2012; IntechOpen. p. 429-456.
  39. Terjung N., et al. Influence of droplet size on the efficacy of oil-in-water emulsions loaded with phenolic antimicrobials. Food Function. 2012; 3(3):290-301.
  40. Burt S. Essential oils: their antibacterial properties and potential applications in foods-a review. International Journal of Food Microbiology. 2004; 94(3):223-253.
  41. Tiwari B.K., et al. Application of natural antimicrobials for food preservation. Journal of Agricultural Food Chemistry. 2009; 57(14):5987-6000.
  42. Delcour A.H. Outer membrane permeability and antibiotic resistance. Biochimica et Biophysica Acta -Proteins Proteomics. 2009; 1794(5):808-816.
  43. Clarke S. Handling, safety and practical applications for use of essential oils. Essential Chemistry for Aromatherapy, 2nd ed.. 2008;231-264.
  44. Idouhli R., et al. Inhibitory effect of Atlas cedar essential oil on the corrosion of steel in 1 m HCl. Corrosion Reviews. 2018; 36(4):373-384.