Production and Biochemical Evaluation of Camelina [Camelina sativa (L.) Crantz ] Doubled Haploid Lines

Document Type : Research Paper

Authors

1 Department of Agriculture, Jouybar Branch, Islamic Azad University, Jouybar, Iran

2 Agricultural Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

3 Sararood Branch, Dryland Agriculture Research Institute (DARI), Agriculture Research, Education & Extension Organization (AREEO), Kermanshah, Iran

Abstract

The medicinal-oil plant Camelina sativa (L.) Crantz belongs to the Brassicaceae family. Various experiments have shown that the plant has much fewer water requirements and more resistance to spring cold than other oilseeds, especially Rapeseed. In this study, 136 camelina double haploid lines from anther culture progeny (F1) of crosses of 27 selected parents from different countries were generated. After ensuring that the lines were double haploid, biochemical markers were used to investigate genetic diversity. In the biochemical markers section, grain oil content, grain protein and type of fatty acids were measured. The experiment was carried out based on a randomized complete block design with four replications. To determine fatty acids using chromatography, 18 types of fatty acids were identified in camelina seed oil. Genetic parameters including phenotypic and genotypic variation coefficients, heritability and genetic advance were estimated. In this study, the highest phenotypic and genotypic variation in fatty acids (C14:0-C16:1) were estimated. Also, the highest general heritability for fatty acids (96.49% for C20:0, 98.92% for C20:2 and 98.59% for C20:3) were assessed. In this reserach, two lines with values of 35.81-36.67% linolenic acid and four lines with values between 22.08-23% of linoleic acid were identified. Also, the ratio of omega 6 to omega 3 ranged between 0.479 0 and 0.759.

Keywords

Main Subjects

References

  1. Abbaszadeh M., Banifatemeh H., Nikdel N. A Study of Farmers’ Perception of Urmia Lake (UL) Crisis with Grounded Theory Approach. Appl Sociol J. 2020; 31(2): 1-22.
  2. Aghmioni M., Aghaei M.J., Vaezi Sh, Majidi Heravan E. Evaluation of genetic diversity, heritability and genetic progress in Kabuli type Chickpea genotypes. Iran J Pulses Res. 2015; 6: 100-107 (In Persian).
  3. Aslani F., Mozaffari J., Ghannadha M., Attari A. Microspore Culture for Producing Haploid Plants in Different Rapeseed (Brassica napus) Cultivars. Iran Agric Sci. 2005; 36 (2).
  4. Bakhshi B., Rostami-Ahmadvandi H., Fanaei H.R. Camelina, an adaptable oilseed crop for the warm and dried regions of Iran. Cent Asian J Plant Sci Innov. 2021; 1(1): 39-45.
  5. Crippa I., Bermejo C., Espósito M.A., Martin E.A., Cravero V., Liberatti D., et al. Genetic variability, correlation and path analyses for agronomic traits in Lentil genotypes. Int J Plant Breed 2009; 3(2): 76-80.
  6. Crowley J.G., Fröhlich A. The composition and use of camelina. Teagasc, Dublin, Ireland. 1998.
  7. Derksen D.A., Anderson R.L., Blackshaw R.E., Maxwell B. Weed dynamics and management strategies for cropping systems in the northern Great Plains. Agron J. 2002; 94(2): 174-185.
  8. Enjalbert J.N., Zheng S., Johnson J.J., Mullen J.L., Byrne P.F., McKay J.K. Brassicaceae germplasm diversity for agronomic and seed quality traits under drought stress. Ind Crops Prod. 2013; 47: 176-185.
  9. Fallah F., Kahrizi D., Rezaeizad A., Zebarzadi A., Zarei L.. Evaluation of Genetic Variation and Parameters of Fatty Acid Profile in Doubled Haploid Lines of Camelina sativa L. Plant Genetic Res J. 2020; 6(2): 79-96.
  10. FAOSTAT. Food and Agriculture Organization of the United Nations Statistics Division. FAO of the United Nations. 2015; http://faostat3.fao.org.
  11. Ferrie A.M.R., Bethune T.D. A microspore embryogenesis protocol for Camelina sativa, a multi-use crop. Plant Cell Tissue Organ Culture (PCTOC). 2011; 106(3): 495-501.
  12. Ferrie A.M.R., Keller W.A. Optimization of methods for using polyethylene glycol as a non-permeating osmoticum for the induction of microspore embryogenesis in the Brassicaceae. In Vitro Cell Dev Biol Plant. 2007; 43(4): 348-355.
  13. Francis A., Warwick S.I. The biology of Canadian weeds. 142. Camelina alyssum (Mill.) Thell.; C. microcarpa Andrz. ex DC.; C. sativa (L.) Crantz. Can J Plant Sci. 2009; 89(4): 791-810.
  14. Gavzan H. A review on health benefits of echium oil; as dietary vegetable source of essential fatty acids. Iran J Physiol Pharmacol. 2019; 2(4): 239-226.
  15. Ghanbari A. Investigation of oilseed cultivation in Iran. Political Deputy and Political Res Department of IRIB. 2019.
  16. Gunstone F.D. ed. Rapeseed and canola oil: production, processing, properties and uses. CRC Press. 2004.
  17. Hakansson S. Weeds and Weed Management on Arable Land An Ecological Approach. CABI. 2003.
  18. Haslam T.M., Kunst L. Extending the story of very-long-chain fatty acid elongation. Plant Sci. 2013; 210: 93-107.
  19. Hoagland D.R., Snyder W.C. Nutrition of strawberry plant under controlled conditions. (a) Effects of deficiencies of boron and certain other elements, (b) susceptibility to injury from sodium salts. Proceed Am Society Hortic Sci 1933; 30: 288–294.
  20. Imbrea F., Jurcoane S., Halmajan H.V., Duda M., Botos L. Camelina sativa: A new source of vegetal oils. Rom. Biotechnol. Lett. 2011; 16(3): 6263-6270.
  21. Jiang Y., Caldwell C.D. Effect of nitrogen fertilization on camelina seed yield, yield components, and downy mildew infection. Can J Plant Sci. 2016; 96(1): 17-26.
  22. Kahrizi D., Maniee M., Mohammadi R., Cheghamirza K. Estimation of genetic parameters related to morpho-agronomic traits of Durum Wheat (Triticum turgidum var. durum). Biharean Biol. 2010; 4(2): 93-97.
  23. Kahrizi D., Rostami A.H., Akbarabadi A. Feasibility cultivation of Camelina (Camelina sativa) as medicinal-oil Plant in Rainfed Conditions in Kermanshah-Iran's First Report. 2015.
  24. Kurasiak-Popowska D., Ryńska B., Stuper-Szablewska K. Analysis of distribution of selected bioactive compounds in Camelina sativa from seeds to pomace and oil. Agronomy. 2019; 9(4): 168.
  25. Liberty J.T., Dehghannya J., Ngadi M.O. Effective strategies for reduction of oil content in deep-fat fried foods: A review. Trends Food Sci Technol. 2019; 92: 172-183.
  26. Lichter R. Induction of haploid plants from isolated pollen of Brassica napus. Zeitschrift für Pflanzenphysiologie. 1982; 105(5): 427-434.
  27. Naghavi M.R., Ghareyazie B., Hosseini Salekdeh Gh. Molecular markers (3 st edn). University of Tehran Press. 2009; pp. 5-6. (In Persian).
  28. Rahman M., de Jiménez M.M. Behind the scenes of microspore-based double haploid development in Brassica napus: A review. Plant Sci Mol Breed J. 2016; 5: 1.
  29. Raziei Z., Kahrizi D., Rostami-Ahmadvandi H. Effects of climate on fatty acid profile in Camelina sativa. Cell Mol Biol. 2018; 64(5): 91-96.
  30. Rezaei A., Hibdi F. Commercializable research achievements of the Agricultural Research, Education and Extension Organization. 2020. Agricultural Research, Education and Extension Organization. Technology Planning and Monitoring Group. The first volume of agriculture and horticulture.
  31. Singh T.P., H.L., Raige J. Kumari Singh, Deshmukh PS. Evaluation of Chickpea genotypes for variability in seed protein content and yield components under restricted soil moisture condition. Indian J Plant Physiol. 2014; 19: 273-280.
  32. Toncea I., Necseriu D., Prisecaru T., Balint L.N., Ghilvacs M.I., Popa M. The seed’s and oil composition of Camelia first Romanian cultivar of camelina (Camelina sativa, L. Crantz). Rom. Biotechnol. Lett. 2013; 18(5): 8594-8602.
  33. USDA N.A.S.S. National Agricultural Statistics Service. USDA. 2015; http://www.nass.usda.gov/ (accessed Dec. 2015).
  34. Watts JL. Using Caenorhabditis elegans to uncover conserved functions of omega-3 and omega-6 fatty acids. Clinic Med J. 2016; 5(2): 19.
  35. Zanetti F., Eynck C., Christou M., Krzyżaniak M., Righini D., Alexopoulou E., et al. Agronomic performance and seed quality attributes of Camelina (Camelina sativa L. crantz) in multi-environment trials across Europe and Canada. Ind Crops Prod. 2017; 107: 602-608.
Journal of Medicinal plants and By-Products
Volume 13, Issue 2
June 2024
Pages 361-368

Files

Share

How to cite

Statistics