Anti-inflammatory and Anti-nociceptive Effects of Teucrium orientale L. Ethanolic Extract in Experimental Animals

Document Type : Research Paper

Authors

1 Pharm D. Student, Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

2 Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

3 Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

Abstract

Background: Teucrium orientale is commonly used as an analgesic, antibacterial, antifungal, and antioxidant, protecting the liver and digestive system, and treating type 2 diabetes.
Purpose: Evaluation of anti-nociceptive and anti-inflammatory properties of ethanolic plant extract using animal models.
Methods: The ethanolic extract was prepared using the maceration method from ground-dried aerial parts of the plant. Analgesic effects were determined by acetic acid-induced writhing and hot plate test on mice. The anti-inflammatory effect was evaluated using Carageenan and Cotton pellet tests on rats.
Result: In the writhing test, TOE (100, 200 and 400 mg/kg) caused a significant reduction in abdominal contractions. Inhibition percentages of abdominal contractions in test groups (100, 200 and 400 mg/kg), morphine and mefenamic acid were 87.40%, 91.80%, 98.29%, 94.40% and 96.89%, respectively, which indicate the visceral analgesic effects.
In hot plate tests, the pain threshold significantly increased in test groups. Extract in the doses of 100mg/kg (P< 0.001), 200mg/kg (P< 0.0001) and 400mg/kg (P< 0.001) significantly reduced the paw edema in the carrageenan test at the second hour. In the cotton pellet test, the prescribed doses of the plant (100-200 mg/kg; P<0.0001) significantly reduced the formation of granuloma tissue and reduced the rate of edema. The percentage of inhibition of granuloma tissue by indomethacin and extract at doses of 100 and 200 mg/kg were 39.19%, 66.75% and 75.49%, respectively, and the percentage of inhibition of exudates were 26.5%, 55.57% and 67.24%, respectively.
Conclusion: These results clearly showed the anti-nociceptive and anti-inflammatory effect of Teucrium orientale extract in animal models.

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References

  1. Mozaffarian V. A dictionary of Iranian plant names. Farhang Mosavar Publ., Tehran, Iran. 2006.
  2. Ulubelen A., Topu G., Sönmez U. Chemical and biological evaluation of genus Teucrium. Stud Nat Prod Chem. 2000;23:591-648.
  3. Miri A., Sharifi-Rad J., Tabrizian K., Nasiri A.A. Antinociceptive and anti-inflammatory activities of Teucrium persicum Boiss. extract in mice. Scientifica. 2015; 1732-1738.
  4. Ghahraman A. Color atlas of Iranian flora. Research Institute of Forests and Rangelands Publishing, Tehran 1996;9:3071.
  5. Dehghan G., Tahmasebpour N., Hosseinpourfeizii M., Sheikhzadeh F., Banan Khojasteh S. Hypoglycemic, antioxidant and hepato-and nephroprotective effects of Teucrium orientale in streptozotocin diabetic rats. Pharmacol online. 2013;1:182-89.
  6. Çakir A., Mavi A., Kazaz C., Yildirim A., KÜFREVİOĞLU Ö.İ. Antioxidant activities of the extracts and components of Teucrium orientale L. var. orientale. Turk J Chem. 2006;30:483-94.
  7. Amiri H. Antioxidant activity of the essential oil and methanolic extract of Teucrium orientale (L.) subsp. taylori (Boiss.) Rech. F. Iran J Pharm Res. 2010;9:417-23.
  8. Küçük M., Güleç C., Yaşar A., Üçüncü O., Yaylι N., Coşkunçelebi K., et al. Chemical Composition and Antimicrobial Activities of the Essential Oils of Teucrium chamaedrys. subsp. chamaedrys., T. orientale. var. puberulens., and T. chamaedrys. subsp. lydium. Pharm Biol. 2006;44:592-99.
  9. Kucukbay F.Z., Yildiz B., Kuyumcu E., Gunal S. Chemical composition and antimicrobial activities of the essential oils of Teucrium orientale var. orientale and Teucrium orientale var. puberulens. Chem Nat Compd. 2011;47:833-36.
  10. Siegmund E., Cadmus R., Lu G. A method for evaluating both non-narcotic and narcotic analgesics. Exp Biol Med. 1957;95:729-31.
  11. Rangriz E., Mousavi Z., Najafizadeh P., Asgarpanah J. Antinociceptive effect of the endemic species glaucium vitellinum boiss and buhse. Jundishapur J Nat Pharm Prod. 2016;11(1)e24829.
  12. Morris C.J. Carrageenan-induced paw edema in the rat and mouse. Inflammation protocols: Springer; 2003. p. 115-21.
  13. Winter C.A., Porter C.C. Effect of alterations in side chain upon anti‐inflammatory and liver glycogen activities of hydrocortisone esters. J the American Pharmaceutical Association 1957;46:515-19.
  14. Lahlou M. The success of natural products in drug discovery. 2013.
  15. Deraedt R., Jouquey S., Delevallée F., Flahaut M. Release of prostaglandins E and F in an algogenic reaction and its inhibition. Eur J Pharmacol. 1980;61:17-24.
  16. Shah S.M.M., Ullah F., Shah S.M.H., Zahoor M., Sadiq A. Analysis of chemical constituents and antinociceptive potential of essential oil of Teucrium Stocksianum bioss collected from the North West of Pakistan. BMC Complement Altern Med. 2012;12:1-6.
  17. Abdollahi M., Karimpour H., Monsef-Esfehani H.R. Antinociceptive effects of Teucrium polium L. total extract and essential oil in mouse writhing test. Pharmacol Res. 2003;48:31-5.
  18. Zendehdel M., Taati M., Jadidoleslami M., Bashiri A. Evaluation of pharmacological mechanisms of antinociceptive effect of Teucrium polium on visceral pain in mice. Iran J Vet Res. 2011; 292-97.
  19. Shah S.M.M., Sadiq A., Shah S.M.H., Ullah F. Antioxidant, total phenolic contents and antinociceptive potential of Teucrium stocksianum methanolic extract in different animal models. BMC Complement Altern Med. 2014;14:1-7.
  20. Winter C.A., Risley E.A., Nuss G.W. Carrageenin-induced edema in hind paw of the rat as an assay for antiinflammatory drugs. Proceedings of the society for experimental biology and medicine 1962;111:544-7.
  21. Brunton L., Chabner B., Knollman B. Goodman and Gilman’s the pharmacological basis of therapeutics, ed 12, New York, McGraw-Hill. 2010.
  22. Chitsaz R., Zarezadeh A., Asgarpanah J., Najafizadeh P., Mousavi Z. Rubiadin exerts an acute and chronic anti-inflammatory effect in rodents. Braz J Biol. 2021;83. e243775.
  23. Jahandar F., Asgarpanah J., Najafizadeh P., Mousavi Z. Anti-inflammatory activity and chemical composition of Pycnocycla bashagardiana fruit’s essential oil in animal models. Iran J Basic Med Sci. 2018;21:188-93.
  24. Spector W. The granulomatous inflammatory exudates. Int Rev Exp Pathol. 1969;8:1-55.
  25. Swingle K., Shideman F. Phases of the inflammatory response to subcutaneous implantation of a cotton pellet and their modification by certain antiinflammatory agents. J Pharmacol Exp Ther. 1972;183:226-34.
  26. Tariq M., Ageel A., Al-Yahya M., Mossa J., Al-Said M. Anti-inflammatory activity of Teucrium polium. Int J Tissue React. 1989;11:185-88.
  27. Rahmouni F., Hamdaoui L., Rebai T. In vivo anti-inflammatory activity of aqueous extract of Teucrium polium against carrageenan-induced inflammation in experimental models. Arch Physiol Biochem. 2017;123:313-21.
  28. Menichini F., Conforti F., Rigano D., Formisano C., Piozzi F., Senatore F. Phytochemical composition, anti-inflammatory and antitumour activities of four Teucrium essential oils from Greece. Food Chem. 2009;115:679-86.
  29. Puntero B.F., Peinado I.I., del Fresno A.M.V. Anti-inflammatory and antiulcer activity of Teucrium buxifolium. J Ethnopharmacol. 1997;55:93-8.
  30. Passos G.F., Fernandes E.S., da Cunha F.M., Ferreira J., Pianowski L.F., Campos M.M., et al. Anti-inflammatory and anti-allergic properties of the essential oil and active compounds from Cordia verbenacea. J Ethnopharmacol. 2007;110:323-33.
  31. De Martino L., Coppola R., De Feo V., Caputo L., Fratianni F., Nazzaro F. Essential oils diversity of Teucrium species. Teucrium Species: Biology and Applications: Springer; 2020.
  32. Zlatić N., Stanković M. Anticholinesterase, Antidiabetic and anti-inflammatory activity of secondary metabolites of Teucrium species. Teucrium Species: Biology and Applications: Springer; 2020.
  33. Uritu C.M., Mihai C.T., Stanciu G.D., Dodi G., Alexa-Stratulat T., Luca A., et al. Medicinal plants of the family Lamiaceae in pain therapy: A review. Pain Res Manag. 2018;2018.
  34. Trivellini A., Lucchesini M., Maggini R., Mosadegh H., Villamarin T.S.S., Vernieri P., et al. Lamiaceae phenols as multifaceted compounds: bioactivity, industrial prospects and role of “positive-stress”. Ind Crops Prod. 2016;83:241-54.
  35. Him A., Ozbek H., Turel I., Oner A.C. Antinociceptive activity of alpha-pinene and fenchone. Pharmacologyonline 2008;3:363-69.
  36. Fidyt K., Fiedorowicz A., Strządała L., Szumny A. β‐caryophyllene and β‐caryophyllene oxide—natural compounds of anticancer and analgesic properties. Cancer med. 2016;5:3007-17.
  37. Chavan M., Wakte P., Shinde D. Analgesic and anti-inflammatory activity of Caryophyllene oxide from Annona squamosa L. bark. Phytomedicine 2010;17:149-51.
  38. Rao V., Menezes A., Viana G. Effect of myrcene on nociception in mice. J Pharm Pharmacol. 1990;42:877-78.
  39. do Amaral J.F., Silva M.I.G., de Aquino Neto M.R.A., Neto P.F.T., Moura B.A., de Melo C.T.V., et al. Antinociceptive effect of the monoterpene R-(+)-limonene in mice. Biol Pharm Bull. 2007;30:1217-20.
  40. de Almeida A.A.C., Silva R.O., Nicolau L.A.D., de Brito T.V., de Sousa D.P., Barbosa A.L.d.R., et al. Physio-pharmacological investigations about the anti-inflammatory and antinociceptive efficacy of (+)-limonene epoxide. Inflammation. 2017;40:511-22.
  41. Peana A.T., Marzocco S., Popolo A., Pinto A. (−)-Linalool inhibits in vitro NO formation: probable involvement in the antinociceptive activity of this monoterpene compound. Life sci. 2006;78:719-23.
  42. Choi Y.W., Kim H.J., Park S.S., Chung J.H., Lee H.W., Oh S.O., et al. Inhibition of endothelial cell adhesion by the new anti-inflammatory agent α-iso-cubebene. Vasc Pharmacol. 2009;51:215-24.
  43. Kim D.S., Lee H.J., Jeon Y.D., Han Y.H., Kee J.Y., Kim H.J., et al. Alpha-pinene exhibits anti-inflammatory activity through the suppression of MAPKs and the NF-κB pathway in mouse peritoneal macrophages. Am J Chin Med. 2015;43:731-42.
  44. Rufino A.T., Ribeiro M., Sousa C., Judas F., Salgueiro L., Cavaleiro C., et al. Evaluation of the anti-inflammatory, anti-catabolic and pro-anabolic effects of E-caryophyllene, myrcene and limonene in a cell model of osteoarthritis. Eur J Pharmacol. 2015;750:141-50.
  45. Aberumand M., Asgarpanah J. Essential oil composition of Teucrium orientale subsp. glabrescens from Iran. Chem Nat Compd. 2017;53:381-82.
Journal of Medicinal plants and By-Products
Volume 13, Issue 2
June 2024
Pages 429-437

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