Comparative HPLC Analysis of 6-Gingerol and 6-Shogaol in Soil-Based and Soilless-Grown Ginger

Document Type : Research Paper

Authors

Centre for Drug and Herbal Development, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia

Abstract

Ginger or Zingiber officinale Roscoe is a well-known herbal medicine and is widely used in Asian cuisine. Its major bioactive compounds, 6-gingerol and its dehydrated form, 6-shogaol, were reported to have potential medicinal properties. However, previous phytochemical studies on the compounds are limited to conventionally grown or soil-based ginger, neglecting soilless ginger grown through hydroponic techniques. This technique has been widely adopted as an alternative to circumvent soil-related complications. Therefore, this study aimed to compare both marker compounds in soil-based (SB) and soilless-grown hydroponic (HP) ginger extracted in different ethanol concentrations (95% and 100%) using high-performance liquid chromatography. The study initially found that 6-gingerol concentration in 95% SB ginger ethanolic extract (1.012%) was significantly higher (p<0.05) than in 95% HP dried ginger (HP1) ethanolic extract (0.314%). The 6-gingerol content for both gingers were also significantly higher (p<0.05) in 95% ethanolic extracts compared to 100% ethanolic extracts. The analysis was also performed with fresh-dried HP ginger (HP2), and it was found that the HP2 ginger (0.75%) has a significantly higher 6-gingerol concentration (p<0.05) compared to HP1 ginger (0.314%), confirming that the previous results were implicated by storage conditions. The concentration of 6-gingerol in 95% SB ginger extract (1.012%) differ significantly compared to those in HP2 extract (0.75%) while both gingers have equivalent amount of low 6-shogaol concentrations (0.0004% and 0.0005% respectively). It is worth to note that HP ginger grown in soilless condition could still produce high amount of 6-gingerol. This finding encourages the usage of HP ginger in pharmacological studies considering the other economic and environmental benefits it offers.

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References

  1. Nafi A., et al. Partial Characterization of an Enzymatic Extract from Bentong Ginger (Zingiber officinale var. Bentong). Molecules. 2014; 19: p. 12336-12348.
  2. Kubra I.R, Rao L.J. An impression on current developments in the technology, chemistry, and biological activities of ginger (Zingiber officinale Roscoe). Crit Rev Food Sci Nutr. 2012; 52(8): p. 651-88.
  3. Kizhakkayil J., Sasikumar B. Diversity, characterization and utilization of ginger: a review. Plant Genetic Resources. 2011; 9(3): p. 464-477.
  4. Committee M.H.M. Malaysian Herbal Monograph. Forest Res Institute Malaysia. 2015; 517-531.
  5. Mao Q.Q., et al. Bioactive Compounds and Bioactivities of Ginger (Zingiber officinale Roscoe). Foods. 2019; 8(6).
  6. Jafarzadeh A., Nemati M. Therapeutic potentials of ginger for treatment of Multiple sclerosis: A review with emphasis on its immunomodulatory, anti-inflammatory and anti-oxidative properties. J Neuroimmunol. 2018; 324: p. 54-75.
  7. Teng H., et al. Comparing the effects of microwave radiation on 6-gingerol and 6-shogaol from ginger rhizomes (Zingiber officinale Rosc). PLoS One. 2019; 14(6): p. e0214893.
  8. Jafarzadeh H., et al., Making sense of COVID-19 over time in New Zealand: Assessing the public conversation using Twitter. PLoS One. 2021; 16(12): p. e0259882.
  9. Ali B.H., et al. Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): a review of recent research. Food Chem Toxicol. 2008; 46(2): p. 409-20.
  10. Kim C.Y., et al. Neuroprotective Effect and Molecular Mechanism of [6]-Gingerol against Scopolamine-Induced Amnesia in C57BL/6 Mice. Evid Based Complement Alternat Med. 2018; 2018: p. 8941564.
  11. Kim J.K., et al. [6]-Gingerol prevents UVB-induced ROS production and COX-2 expression in vitro and in vivo. Free Radic Res. 2007; 41(5): p. 603-14.
  12. Qian Q.H., et al. Effect of gingerol on substance P and NK1 receptor expression in a vomiting model of mink. Chin Med J (Engl). 2010; 123(4): p. 478-84.
  13. Shukor N.I.A., et al. The changes of chemical quality of ginger during postharvest storage at chilling temperature. Food Res. 2020; 4(5): p. 1653-1662.
  14. Yaseer S.M, Mohamad A.M. Commercial ginger production using fertigation system. Bulletin Teknol MARDI. 2012; (1): p. 97-105.
  15. Yaseer S.M., et al. Effects of Organic Substrates on Growth and Yield of Ginger Cultivated Using Soilless Culture. Malays. Appl. Bio. 2015; 44(3): p. 63-68.
  16. Srivastava V., et al. Agroecological Responses of Heavy Metal Pollution with Special Emphasis on Soil Health and Plant Performances. Frontiers in Environmental Sci. 2017; 5.
  17. Sambo P., et al. Hydroponic Solutions for Soilless Production Systems: Issues and Opportunities in a Smart Agriculture Perspective. Front Plant Sci. 2019; 10: p. 923.
  18. Guideline I.H.T. Validation of analytical procedures: Text and Methodology. Q2 (R1). 2005; 1(20): p. 05.
  19. Kajsongkram T., et al. Development and Validation of a HPLC Method for 6-Gingerol and 6-Shogaol in Joint Pain Relief Gel Containing Ginger (Zingiber officinale). Int J Med and Health Sci. 2015; 9(12).
  20. Khalib S.C. Development and Validation of a HPLC-UV Method for Standardization of Zingiber Cassumunar Roxb. Based on Selected Chemical Markers, in FACULTY OF PHARMACY. UNIVERSITI KEBANGSAAN MALAYSIA. p. 84.
  21. Watson D.G. Pharmaceutical Analysis: A Textbook for Pharmacy Students and Pharmaceutical Chemists. 2 ed. 2012; London: Churchill Livingstone.
  22. Ok S., Jeong W.S. Optimization of Extraction Conditions for the 6-Shogaol-rich Extract from Ginger (Zingiber officinale Roscoe. Prev Nutr Food Sci. 2012; 17: p. 166-171.
  23. Sharif M.F., Bennett M.T. The effect of different methods and solvents on the extraction of polyphenols in ginger (Zingiber officinale). J Teknol (Sci Engin). 2016; 78(11-2): p. 49-54.
  24. Hawlader M.N.A., Perera C.O, Tian M. Comparison of the Retention of 6-Gingerol in Drying of Ginger Under Modified Atmosphere Heat Pump Drying and other Drying Methods. Drying Technol. 2006; 24(1): p. 51-56.
  25. Zhang X., et al. Gingerol Decreases after Processing and Storage of Ginger. J Food Sci. 1994; 59(6).
  26. Wills R., et al. Physiology and biochemistry. Postharvest: An introduction to the physiology and handling of fruit, vegetables and ornamentals. 2007; p. 28-51.
  27. Asamenew G., et al. Characterization of phenolic compounds from normal ginger (Zingiber officinale Rosc.) and black ginger (Kaempferia parviflora Wall.) using UPLC–DAD–QToF–MS. European Food Res Technol. 2019; 245: p. 653-665.
  28. Bailey-Shaw Y.A., et al. Changes in the contents of oleoresin and pungent bioactive principles of Jamaican ginger (Zingiber officinale Roscoe.) during maturation. J Agric Food Chem. 2008; 56(14): p. 5564-5571.
  29. Jiang H., et al. Characterization of gingerol‐related compounds in ginger rhizome (Zingiber officinale Rosc.) by high‐performance liquid chromatography/electrospray ionization mass spectrometry. Rapid Communications in Mass Spectrometry: An Int J Devoted to the Rapid Dissemination of Up‐to‐the‐Minute Res in Mass Spectrometry. 2005; 19(20): p. 2957-2964.
  30. Sanwal S.K., et al. Antioxidant phytochemicals and gingerol content in diploid and tetraploid clones of ginger (Zingiber officinale Roscoe). Scientia Horticulturae. 2010; 124(2): p. 280-285.
  31. Ahui M.L.B., et al. Identification of gingerols in ginger (Zingiber officinale Rosc.) by high-performance liquid chromatography-tandem mass spectrometry and pharmacologic studies of its aqueous extract on the rabbit isolated duodenum contractility. J Phys Pharm Adv. 2013; 3: p. 16-26.
  32. Tanweer S., et al. Comparison and HPLC quantification of antioxidant profiling of ginger rhizome, leaves and flower extracts. Clinical Phytoscience. 2020; 6(12).
  33. Ko M.J., Nam H.H, Chung M.S. Conversion of 6-gingerol to 6-shogaol in ginger (Zingiber officinale) pulp and peel during subcritical water extraction. Food Chem. 2019; 270: p. 149-155.
  34. Yaseer S.M., Adzemi M.A, Zaliha W. Effect of Organic Substrates On Ginger Growth, Yield and [6]-Gingerol Content Cultivated Using Soilless Culture System. Agrofor. 2018; 3.
  35. Sida S., Samakradhamrongthai R., Utama-ang N. Influence of maturity and drying temperature on antioxidant activity and chemical compositions in ginger. Current Applied Sci and Technol. 2019; 19: p. 28-42.
  36. Vedashree M., et al. Characterization of volatile components from ginger plant at maturity and its value addition to ice cream. J Food Sci Technol. 2020; 57(9): p. 3371-3380.
  37. El-Kazzaz A. Soilless Agriculture a New and Advanced Method for Agriculture Development: an Introduction. Agric Res Technol: Open Access J. 2017; 3(2).
Journal of Medicinal plants and By-product
Volume 12, Issue 4
December 2023
Pages 319-328

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