Growth Characteristics and Changes in the Active Ingredients of Salvia mirzayanii Essential oil under Foliar Application of Humic Acid and Amino Acid

Document Type : Research Paper

Authors

1 Department of Horticulture, Jahrom Branch, Islamic Azad University, Jahrom,Iran

2 Department of Natural Resources, Fars Agricultural and Natural Resources Research and Education Center, AREEO,Shiraz,Iran

Abstract

Salvia mirzayanii Rech.f. & Esfand. is a medicinal plant from the Lamiaceae family, which has long been of interest to the industry due to its medicinal properties and various metabolites, including terpenoids, phenolic compounds, flavonoids, fatty acids, and sterols. This study was conducted to investigate the effect of foliar spraying of amino acid with concentrations of 0, 0.5, 1, and 1.5 ml/l and humic acid with concentrations of 0, 5, 10, and 15 gr/l along with irrigation water on essential oil compounds and plant growth. The results showed that humic acid (15 ml/l) and amino acid (0.5 ml/l) increased fresh weight, dry weight of leaves, number of inflorescences, and weight of inflorescences, and a further increase in the amino acid level caused a decrease in the measured agricultural characteristics. The use of humic acid 15 mgr/l caused the increase of 5-neo-sedranol, α-terpinyl acetate, bicyclogermacrene, and linalyl acetate as the main components of the essential oil to the extent of 37.5, 20.6, 32.5, 94.1 and 22.7% compared to the control respectively. The amount of 5-neo-sedranol and bicyclogermacrone decreased with the increase of amino acid. Amino acid treatment of 1.5 ml/l increased the percentage of linalyl acetate (40.98%), α-terpinyl acetate (18.06%), and linalool (10.71%) compared to the control. According to the trend of changes in the percentage of essential oil compounds and the measured agricultural characteristics, it is suggested to use the level of 0.5 mg amino acid and 15 mg of humic acid to reach the most desirable quantitative and qualitative yield of Salvia mirzayanii.

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  1. Sánchez-Mata D., Morales R. The Mediterranean Landscape and Wild Edible Plants. Mediterranean Wild Edible Plants. 2016.
  2. Ramasubramania R. Medicinally potential plants of Labiatae (Lamiaceae) Family: An Overview. J. Med. Plant Res. 2012; 6: 203-213.
  3. McKay D., Blumberg, J. A review of the bioactivity and potential health benefits of peppermint tea (Mentha piperita L.). Phytother. Res. 2006; 20: 619-633.
  4. Jamzad Z. A survey of Lamiaceae in the flora of Iran. Rostaniha. 2013; 14: 59-67.
  5. Moazzami Frida H., Rajabi T., Salami A., Ranjbar M., Taghizadeh M., Rahmani N. Fatty acid composition and phytosterol contents of the seeds from three Salvia L. Species Growing in Iran. Biol. Sci. Promot. 2015; 28: 421-434.
  6. Mohammadian R., Dehghan G., Movafeghi A., Talebpour A. Phytochemical study of Salvia sahendica in different growth stages. Master's Thesis, Faculty of Natural Sciences, Uni. Tabriz. 2014.
  7. Saito B., Seckler M. Alkaline Extraction of Humic Substances from Peat Applied to Organic-Mineral Fertilizer Production. Braz. J. Chem. Eng. 2014; 31(3): 675-682.
  8. Nikbakht A., Kafi M., Babalar M., Xia Y., Luo A. Etemadi N. Effect of Humic Acid on Plant Growth, Nutrient Uptake, and Postharvest Life of Gerbera. J. Plant Nutr. 2008; 31: 1065-1080.
  9. Cacilia R., Juarez R., Craker L., Mendoza, A. Humic substances and moisture content in the production of biomass and bioactive constituents of Thyme (Thymus vulgaris L.). Articulocientifico. 2011;34(3): 183-188.
  10. Khazaie H., EyshiRezaie E., Bannayan M. Application times and concentration of humic acid impact above-ground biomass and oil production of hyssop (Hyssopus officinalis). Med. Plant Res. 2011;5(20): 5148- 5154.
  11. Azizi M., Safaei Z. The effect of foliar application of humic acid and nano fertilizer (pharmks®) on morphological traits, yield, essential oil content and yield of black cumin (nigella sativa l.). J. Hortic. Sci. (Agric. Sci.). 2017; 30(4): 671-680.
  12. Haghighi M., Mozaffarian M. Application of amino acids on the growth and yield of tomatoes and greenhouse bell peppers. Two Q. J. Vegetable Sci. 2015; 1: 69-59.
  13. Gawronak H. Biostimulators in modern agriculture (general aspects). Arysta Life Science. Warsaw: Editorial House Wies Jutra, Limited. 2008.
  14. Faten S., Shaheen A., Ahmad A., Mahmoud A. Effect of foliar application of amino acids as antioxidants on growth, yield and characteristics of squash. Am. J. Agric. Biol. Sci. 2010; 6(5): 583-588.
  15. Thomas J., Mandal A., Raj Kumar R., Chrodia A. Role of biologically active amino acid formulations on tea quality and crop productivity (Camelia sp.). Int. J. Agric. Res. 2009; 4: 228-236.
  16. Golzadeh H., Mehrafarin A., Naghdi Badi H., Fazeli F., Ghaderi A., Zarincheh N. Effects of bio-stimulants on quantitative and qualitative yield of German chamomile. J. Med. Plant. 2011; 11(41): 195-207.
  17. Entezari S., Khalatbari M., Nasri M., Zakeri M., The effect of amino acid spraying on water deficit in wheat in varamin condition. Plant Ecosystem. 2008; 4(14): 64-76.
  18. Sharma, A. A handbook on organic farming. India: Agrobios.2002.
  19. Palada M., Crossman S., Kowalski J. Growth and yield response of thyme (Thymus vulgaris L.) to the source of nitrogen fertilizer. Caribbean Food Crops Soc.1994. 30; 58-64.
  20. Adams, R. Identification of Essential Oils by Ion Trap Mass Spectroscopy. New York: Academic Press. 2011.
  21. Davies N. Gas chromatographic retention indices of monoterpenes and sesquiterpenes on methyl silicone and Carbowax 20. M phases. J. Chromatogr. 1998; 503: 1-24.
  22. Shibamoto T. Retention indices in essential oil analysis. Sandra, P. and Bichi, C. (eds.) Capillary Gas Chromatography in Essential Oil Analysis (pp. 259-274). New York: Alfred Heuthig. 1987.
  23. Befrozfar M., Habibi D., Asgharzadeh A., Sadeghi- Shoae M., Tookalloo M. Vermicompost, plant growth promoting bacteria and humic acid can affect the growth and essence of sweet basil (Ocimum basilicum L.). Ann. Biol. Res. 2013; 4(2): 8-12.
  24. Khoramdel S., Kuchaki A., NasiriMahalati M., Ghorbani R. Effect of biofertilizers on growth indices of black cumin (Nigella sativa L.). J. Agric. Res. 2008; 6: 294-285.
  25. Mirhajian A. What is humic acid. Agric. Eng. 2012;33:7-16.
  26. Delfine S., Tognetti R., Desiderio E., Alvino A. Effect of foliar application of N and humic acids on growth and yield of durum wheat. Agron. Sustain. Dev. 2005; 25:183-191.
  27. Ghorbani S., Khazayi H., Kafi M., Banayanaval, M. Effect of Humic acid in irrigation water on yield and yield components of maize (Zea mays L.). J. Ecol. 2010;2(1): 131-123.
  28. Ayas H., Gulser F. Use of humic acid for improving soil organic matter and increasing crop yield. Res. Biol. Sci. 2005; 5(6): 801-804.
  29. Cangi R., Tarakcioglu C., Yasar H. Effect of humic acid applications on yield, fruit characteristics and nutrient uptake in Ercis grape ( L.) cultivar. Chem. Asian J. 2006; 18:1493-1499.
  30. Yu-kui R., Yunfeng P., Zhengrui W., Jian B. Stem perimeter, height and biomass of maize (Zea mays L.) grown under different N fertilization regimes in Beiging. Int. J. Plant Product. 2009;3(2):85-90.
  31. Gijo´n , Guerrero J., Couceiro J., Morian A. Deficit irrigation without reducing yield or nut splitting in pistachio (Pistacia vera cv. Kerman on Pistacia terebinthus L.). Agric. Water Manag. 2009; 96: 12-22.
  32. 32- Bayat H., Nemati H., Selah varzi Y., Abdollahi Saadabad A. The effect of silicon on growth, evaporation and transpiration and reproductive function of Iranian Atlasi (Petunia hybrida). Plant Ecol. J. 2012; 8(33): 51-60.
  33. Filner P. Regulation of nitrate reductase in cultured tobacco cells. Biochimica et Biophysica Ada.1996;118: 299-310.
  34. Gamborg O., Miller R., Ogtma K. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res.1970;50:151-158.
  35. Nardi S., Pizzeghello D., Muscolo A., Vianel, A. Physiological effects of humic substances on higher plants. Soil Biol. Biochem. 2002; 34:1527-1536.36. Omidbeigi R. Production and processing of medicinal plants. Vol. 3. Astan Quds Razavi Publications. 2012.
  36. Sangwan S., Farooqi A., Shabih F., Sangwan S. Regulation of essential oil production in plants. Plant Growth Regulation. 2001;31: 3-21.
  37. Niakan M., Khavarinejad R., Rezaei M. The effect of different ratios of N, P, K fertilizers on fresh weight, dry weight, leaf area and essential oil content of peppermint plant Mentha piperita L. Research of medicinal and aromatic plants of Iran. 2013;20(2):148-131
  38. Gorgini Shabankareh H., Sabouri F., Saedi F., Fakheri B. Effects of different levels of humic acid on growth indices and essential oil of lemon balm (Melissa Officinalis L.) under different irrigation regimes. J. Crop Sci. Res. Arid Reg. 2017;(2):166-176.
  39. Narimani R., Moghaddam M., Ghasemi Pirbalouti P. Phytochemical changes of Dracocephalum moldavica L. Essential oil under different salinity stresses and application of humic and ascorbic acid. Eco-phytochem. J. Med. Plant. 2002;7(4):34-48.
  40. Shahhosseini R., Omidbaigi R., Kiani D. Effect of biological fertilizers of biosulfur, nitroxin and super absorbent polymer on growth, yield and essential oil content of sweet basil (Ocimum basilicum L.). J. Hortic. Sci. (Agric. Sci. Technol.). 2012;26(3):246-254.
  41. Rowshan V., Tarakemeh A. Comparison of S. mirzayanii volatile compounds extracted by headspace extraction and hydrodistillation methods. Int. J. Plant Animal Env. Sci. 2013;3:136-140.
  42. Haghighat M., Alizadeh A., Nouroznejadfard M. Essential oil composition and antimicrobial activity in Iranian S. mirzayanii Rech. and Esfand. Adv. Environ. Biol. 2012; 6: 1985-1989.
  43. Ziaei A., Ramezani M., Wright L., Paetz C., Schneider B., Amirghofran Z. Identification of spathulenol in S. mirzayanii and the immunomodulatory effects. Phytotherapy Res. 2011;25:557-562.
  44. Zarshenas M., Krenn L. Phytochemical and pharmacological aspects of S. mirzayanii Rech. and Esfand. Evid. Based Complement. Alternat. Med. 2015; 20(1):65-72.
  45. Khajeh M., Ghanbari A. Application of factorial design and BoxBehnken matrix in optimizing a microwave-assisted extraction of essential oils from S. mirzayanii. Nat. Prod. Res. 2011; 25: 1766-1770.
  46. Yamini Y., Khajeh M., Ghasemi E., Mirza M., Javidnia K. Comparison of essential oil compositions of S. mirzayanii obtained by supercritical carbon dioxide extraction and hydrodistillation methods. Food Chem. 2008;108:341-346.
  47. Omer E., Said-Al Ahl H., El Gendy E., Wahby M. Effect of amino acids application on production, volatile oil and chemical composition of chamomile cultivated in saline Soil at sinai. Res. J. Appl. Sci. 2013;9:3006-3021.