Anti-diabetic Effects of Caucasian whortleberry and Ferula Extract on Blood Biochemical Factors, Liver and Kidney Histology in Streptozotocin-induced Diabetic Mice

Document Type : Research Paper

Authors

1 Department of Biology, Faculty of Basic Sciences, Hakim Sabzevari University, Sabzevar, Iran

2 Department of Electrical Engineering, Faculty of Engineering, Ferdowsi University of Mashhad, Mashhad, Iran

3 Department of Biology, Sabzavar Branch, Islamic Azad University, Sabzavar, Iran

Abstract

One of the biggest issues facing modern societies is diabetes, which is becoming more prevalent as a result of changes in daily routines. Numerous studies are currently being conducted to determine the impact of herbal medications on diabetes, given the negative side effects of chemical treatments used to treat the condition. The present study aimed to compare the antidiabetic effects of Caucasian whortleberry (Vaccinium arctostaphylos L.) methanolic extract and Ferula brewed extract (Ferula tea). To this end, 40 mice were given 200 mg/kg of streptozotocin intraperitoneally to induce diabetes in them. The mice were then divided into six groups, each consisting of ten mice: Group 1 included healthy mice that did not receive any treatment and was mentioned as the control; Group 2 was considered the diabetic control group and received a daily treatment of water; Group 3 and 4 received a daily treatment of the chemical drugs Acarbose or Metformin, which are regularly and separately used at a dose of 100 mg/kg of each drug. Group 5 received a daily treatment of 250 mg/kg of Caucasian whortleberry methanolic extract, and group 6 received a daily treatment of 300 mg/kg of Ferula tea. After the 40-day trial, the results showed that Caucasian whortleberry methanolic extract, Ferula tea, and Acarbose significantly reduced serum glucose levels compared to the control. In addition, Caucasian whortleberry methanolic extract caused a significant reduction in triglycerides and alkaline phosphatase.
Abbreviations: STZ: Streptozotocin, HDL: High-Density Lipoproteins, LDL: Low-Density Lipoproteins, SGOT: Serum Glutamic Oxaloacetic Transaminase, ALP: Alkaline Phosphatase, HbA1c: Hemoglobin A1c, SGPT: Serum Glutamic Pyruvic Transaminase, CGA: Chlorogenic Acid. FBS: Fast Blood Sugar.

Keywords

Main Subjects

References

  1. Sreenivasamurthy L. Evolution in Diagnosis and Classification of Diabetes. J. Diabetes Mellit. 2021; 11:200-7.
  2. Teshome A.A., Baih S.Z., Wolie A.K., Mengsti M., Muche Z.T., Amare SN., Abdu seid M., Yitbark G.Y., Melkamu Molla Y., Baye N.D., Ayehu GW. Magnitude of impaired fasting glucose and undiagnosed diabetic mellitus and associated risk factors among adults living in Woreta town, northwest Ethiopia: a community-based cross-sectional study, 2021. BMC Endocr Disord. 2022; 22:243.
  3. Adams J.N., Cox A.J., Freedman B., Langefeld C.D., Carr J.J., Bowden D.W. Genetic analysis of haptoglobin polymorphisms with cardiovascular disease and type 2 diabetes in the diabetes heart study. Cardiovasc Diabetol. 2013; 12:31.
  4. Alanis AY., Sanchez O.D., Vaca-González A., Rangel-Heras E. Intelligent Classification and Diagnosis of Diabetes and Impaired Glucose Tolerance Using Deep Neural Networks. Mathematics. 2023; 11(19):4065.
  5. Pei J., Wang B., Wang D. Current Studies on Molecular Mechanisms of Insulin Resistance. J. Diabetes Res. 2022; 2022: 1863429.
  6. Choi K., Kim Y.B. Molecular mechanism of insulin resistance in obesity and type 2 diabetes. The Korean J Intern Med. 2010; 25(2):119-29.
  7. Rang H.P., Dale M.M., Ritter J.M. The endocrine system pharmacology. Pharmacology. Longman Group Ltd., UK. 1991; 504-8.
  8. El Far M.M, Taie HA. Antioxidant activities, total anthocyanins, phenolics and flavonoids contents of some sweetpotato genotypes under stress of different concentrations of sucrose and sorbitol. Australian Journal of Basic and Applied Sciences. 2009;3(4):3609-16.
  9. Ozkan G, Ercisli S, Alam ZE, Guleray AG, Sagbas HI, Ilhan G. Some morphological and biochemical characteristics of wild grown Caucasian Whortleberry (Vaccinium arctostaphylos L.) genotypes from Northeastern Turkey. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2019;47(2):378-83.
  10. Barut B., Barut E.N., Engin S., Özel A., Sezen F.S. Investigation of the Antioxidant, α-Glucosidase Inhibitory, Anti-inflammatory, and DNA Protective Properties of Vaccinium arctostaphylos L. Turk J Pharm Sci. 2019;16(2):175-83.
  11. Li S., Yuan W., Wang P., Su Z. Anthocyanins and Flavonoids of Vaccinium L. Pharmaceutical Crops 2012; 3: 7-37.
  12. Abidov M., Ramazanov A., Jimenez Del Rio M., Chkhikvishvili I. Effect of Blueberin on fasting glucose, C-reactive protein and plasma aminotransferases, in female volunteers with diabetes type 2: double-blind, placebo controlled clinical study. Georgian Medical News. 2006;1(141):66-72.
  13. Mozaffarian V. A dictionary of Iranian plant names: Latin, English, Persian. Farhang Mo'aser; 1996.
  14. Tamemoto K, Takaishi Y, Chen B, Kawazoe K, Shibata H, Higuti T, Honda G, Ito M, Takeda Y, Kodzhimatov OK, Ashurmetov O. Sesquiterpenoids from the fruits of Ferula kuhistanica and antibacterial activity of the constituents of F. kuhistanica. Phytochemistry. 2001;58(5):763-7.
  15. Shahverdi A.R., Iranshahi M., Mirjani R., Jamalifar H., Amin G., Shafiee A. Bioassay-guided isolation and identification of an antibacterial compound from Ferula persica var. persica roots. DARU- J. Pharm Sci. 2005;13(1): 17-9.
  16. Vafaei A., Sajadi A., Taherian A., Emami-Abarghoi M., Miladi-Gorgi H., Jarrahi M. The effect of Ferula persica on modulation of withdrawal syndrome sign in morphine-dependent mice. Iranian J Pharm Res. 2004;3(1):72.
  17. Alkhatib R., Hennebelle T., Joha S., Idziorek T., Preudhomme C., Quesnel B., Sahpaz S., Bailleul F. Activity of elaeochytrin A from Ferula elaeochytris on leukemia cell lines. Phytochemistry. 2008;69(17):2979-83.
  18. Hamdan I.I., Afifi F.U. Studies on the in vitro and in vivo hypoglycemic activities of some medicinal plants used in treatment of diabetes in Jordanian traditional medicine. J Ethnopharmacol. 2004; 93(1):117-21.
  19. Eizadifard F., Tafrihi M., Mohadjerani M. Antioxidant, cytotoxic, and genotoxic potentials of the gum of Ferula gummosa Boiss on PC-3 cells. Avicenna J Phytomed. 2023;13(3):316-27.
  20. Rasekh A. Topical estrogen accelerates wound healing in diabetic rats. Iranian Journal of Endocrinology and Metabolism. 2011;15;12(5):544-51.
  21. Furman B.L. Streptozotocin-Induced Diabetic Models in Mice and Rats. Current Protocols. 2021; 1(4):e78.
  22. Palsamy P, Subramanian S. Resveratrol protects diabetic kidney by attenuating hyperglycemia-mediated oxidative stress and renal inflammatory cytokines via Nrf2–Keap1 signaling. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease. 2011;1812(7):719-31.
  23. Ramzy M.M., El-Sheikh A.A., Kamel M.Y., Abdelwahab S.A., Morsy M.A. Mechanism of testicular protection of carvedilol in streptozotocin-induced diabetic rats. Indian journal of pharmacology. 2014;46(2):161.
  24. Nasiri Bezenjani S., Razavizadeh R., Oloumi H. Evaluation of content of phenylpropanoid compounds of latex and chemical composition of essential oil of Ferula assa-foetida L. in some natural pasturelands of Kerman, Iran. Journal of Plant Researches (Iran Biology). 2017;3(30):1-4.
  25. Kweon M.H., Hwang H.J., Sung H.C. Identification and antioxidant activity of novel chlorogenic acid derivatives from bamboo (Phyllostachys edulis). Journal of agricultural and food chemistry. 2001;49(10):4646-55.
  26. Meng S., Cao J., Feng Q., Peng J., Hu Y. Roles of chlorogenic acid on regulating glucose and lipids metabolism: a review. Evidence-based complementary and alternative medicine: eCAM. 2013;2013.
  27. Tousch D., Lajoix A.D., Hosy E., Azay-Milhau J., Ferrare K., Jahannault C., Cros G., Petit P. Chicoric acid, a new compound able to enhance insulin release and glucose uptake. Biochemical and biophysical research communications. 2008;377(1):131-5.
  28. Faraji H. Effect of Decaffeinated Coffee-enriched Chlorogenic Acid on Blood Glucose Levels in Healthy Controls: A Systematic Review. Int J Prev Med. 2018; 24; 9.
  29. Meng S., Cao J., Feng Q., Peng J., Hu Y. Roles of chlorogenic acid on regulating glucose and lipids metabolism: a review. Evidence-based complementary and alternative medicine: eCAM. 2013;2013.
  30. Jian-hui W.A., Yong-le L.I., Chi-ling L.I., Jian Y.U., Fa-xiang W.A., Xiang-hong L.I., Yan L.I. Effect of Chlorogenic Acid Extracted from Eucommia ulmoides Oliv. on Hemorheological Changes of Mice Induced by High Fat Diet. NATURAL PRODUCT RESEARCH AND DEVELOPMENT. 2012;23(09):1186.
  31. Ahn T., Yun C.H., Oh D.B. Tissue-specific effect of ascorbic acid supplementation on the expression of cytochrome P450 2E1 and oxidative stress in streptozotocin-induced diabetic rats. Toxicology letters. 2006;166(1):27-36.
  32. Mohamed J., Nafizah A.N., Zariyantey A.H., Budin S. Mechanisms of diabetes-induced liver damage: the role of oxidative stress and inflammation. Sultan qaboos university medical journal. 2016;16(2):e132.
  33. Hajinezhad M.R., Esmaeilzadeh Bahabadi S., Miri H.R., Davari S.A., Darvish Sargazi M. Effect of hydroalcoholic extract of Prosopis farcta pod on liver histopathology and malondialdehyde level in streptozotocin diabetic rats. Ofogh-e-Danesh. 2015;21(1):31-6.
  34. SM BK, Basirat E., Sheikhzadeh F., Hatami H. Effect of alcoholic extract of Rosa canina on hepatic tissue and hepatic enzymes activity in diabetic rats. J. Ardabil University of Medical Sci. 2017;17(1):81-9.
  35. Darenskaya M.A., Kolesnikova L.I., Kolesnikov S.I. Oxidative stress: pathogenetic role in diabetes mellitus and its complications and therapeutic approaches to correction. Bulletin of experimental biology and medicine. 2021;171(2):179-89.
  36. Khaje Gandomani A., Fatahian Dehkordi R.A., Heidarnejad M.S., Jafarian Dehkordi M. Evaluation of serum biochemical and histopathological changes in mice-diabetic kidney followed by simultaneous injection of nanoparticles of zinc oxide and thiamine. Journal of Arak University of Medical Sciences. 2017;20(5):103-18.
  37. Pooladvand V., Taghavi S.M., Mahmoodi M., Tavakolian Ferdosieyeh V., Hosseini Zijoud M. Histological alterations due to the consumption of different doses of Citrullus colocynthis fruit in normal and diabetic male rats. Journal of Mazandaran University of Medical Sciences. 2011;21(82):63-71
Journal of Medicinal plants and By-Products
Volume 13, Issue 2
June 2024
Pages 369-379

Files

Share

How to cite

Statistics