The Phytochemical Content and Cytotoxic Effects of Quinoa Commercial Cultivars (Chenopodium quinoa Willd.) on MCF7 Cell Line of Breast Cancer

Articles in Press

Document Type : Research Paper

Authors

1 Department of Arid Land and Desert Management, School of Natural Resources and Desert Studies, Yazd University, Yazd, Iran

2 Biology Department, Payame Noor University, Tehran, Iran

3 Department of Horticulture, Faculty of Agriculture, Shahed University, Tehran, Iran

4 Department of Medicinal Plants, Research Institute of Forest and Rangelands, Tehran, Iran

Abstract

The Black, Titicaca, and Multi-hued Bulk cultivars were cultivated in the arid and semi-arid regions of Iran to compare the phenolic compounds, antioxidant, and cytotoxic effects of seed and leave extracts of Quinoa on the MCF7 cell line of breast cancer (at 100, 250, 500, and 1000 mg/ml concentrations). The bioactive compounds of samples were extracted using the hydroethanolic solvent using the Soxhlet method. According to this research, although the Titicaca seed extract had the highest total phenolic content at 1000 mg/ml (152 mg GAE/g), the highest average phenolic content was observed in the leaf extracts of all cultivars. So, the seed extract of the Black cultivar showed the highest flavonoid content at 1000 mg/ml (268 mg QE/g DW). The most increased DPPH radical scavenging activity was observed in the leaf extract of the Black cultivar (54.23%), and the highest ferric-reducing power was exhibited by Titicaca leaf extract at 1000 mg/ml (0.37 mmol Fe2+). The results indicated that the leaf extract of the Black cultivar had the lowest IC50 value (786.95 mg/ml). Based on the results, the leaf and seed extracts of Black and Titicaca cultivars were selected to examine the cytotoxic effects of Quinoa extracts on the MCF7 cell line due to their higher phenolic and antioxidant contents. The lowest MCF7 cell line viability percentage (13.92%) was observed in the leaf extract of the Black cultivar at 1000 mg/ml concentration after 72 hours. Regardless of the extract concentration, the leaf and Black cultivar were the superior organ and cultivar compared to other studied characteristics, respectively. In addition, 72 hours significantly yielded better results than other periods.

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References

  1. Torre L.A., Bray F., Siegel R.L., Ferlay J., Lortet-Tieulent J., Jemal A. Global cancer statistics- 2012. CA: Cancer J Clin. 2015; 65(2): 87-108.
  2. Hiller T.W., O’Sullivan D.E., Brenner D.R., Peters C.E., King W.D. Solar ultraviolet radiation and breast cancer risk: a systematic review and meta-analysis. Environ Health Perspect. 2020; 128(1): 016002.
  3. Maryanaji Z. The effect of climatic and geographical factors on breast cancer in Iran. BMC Res Notes. 2020; 13(1): 1-5.
  4. Bodai B. I., Tuso P. Breast cancer survivorship: a comprehensive review of long-term medical issues and lifestyle recommendations. Perm J. 2015; 19(2): 48-79.
  5. Giaquinto A.N., Sung H., Miller K.D., Kramer J.L., Newman L.A., Minihan A., Siegel R. L. Breast cancer statistics, 2022. CA: Cancer J Clin. 2022; 72(6): 524-541.
  6. Faryabi R., Daneshi S., Davarani E.R., Yusefi A.R., Arabpour M., Ezoji K., Hussaini S.M. The assessment of risk factors and risk perception status of breast cancer in Northern Iran. BMC Women's Health. 2023; 23(1): 1-7.
  7. Noel B., Singh S. K., Lillard Jr J. W., Singh R. Role of natural compounds in preventing and treating breast cancer. Front Biosci (Schol Ed). 2020; 12: 137-160.
  8. Ijaz S., Akhtar N., Khan M.S., Hameed A., Irfan M., Arshad M.A., Asrar M. Plant derived anticancer agents: A green approach towards skin cancers. Biomed. Pharmacother. 2018; 103: 1643-1651.
  9. Hazafa A., Rehman K. U., Jahan N., Jabeen Z. The role of polyphenol (flavonoids) compounds in the treatment of cancer cells. Nutr Cancer. 2020; 72(3): 386-397.
  10. Pathan S., Siddiqui R.A. Nutritional composition and bioactive components in quinoa (Chenopodium quinoa Willd.) greens: A review. Nutrients. 2022; 14(3): 1-12.
  11. Pilco-Quesada S., Tian Y., Yang B., Repo-Carrasco-Valencia R., Suomela J.P. Effects of germination and kilning on the phenolic compounds and nutritional properties of quinoa (Chenopodium quinoa) and kiwicha (Amaranthus caudatus). J Cereal Sci. 2020; 94: 1-7.
  12. Rahimi E., Bagheri M. Chemical, antioxidant, total phenolic and flavonoid components and antimicrobial effects of different species of quinoa seeds. Egypt J Vet Sci. 2020; 51(1): 43-54.
  13. Camaggio G., Amicarelli V. The ancient crop of quinoa for world food security. In: Micniakiewicz M. & Popek S (eds). Society of Commodity Science, Cracow University. 2014; 1: 31-45.
  14. Song J., Shao Y., Chen X., Li.X. Release of characteristic phenolics of quinoa based on extrusion technique. Food Chem. 2022; 374: 128780.
  15. Chen X., He X., Sun J., Wang Z. Phytochemical composition, antioxidant activity, α-glucosidase and acetylcholinesterase inhibitory activity of quinoa extract and its fractions. Molecules. 2022; 27(8): 1-16.
  16. Hu Y., Zhang J., Zou L., Fu C., Li.P., Zhao G. Chemical characterization, antioxidant, immune-regulating and anticancer activities of a novel bioactive polysaccharide from Chenopodium quinoa seeds. Int J Biol Macromol. 2017; 99: 622-629.
  17. Albadi I. A. The effect of Chenopodium quinoa saponins on the proliferation of MCF-7 and MDA-MB-231 breast cancer (Doctoral dissertation, Near East University). 2020; 1-53.
  18. Nwozo O.S., Effiong E.M., Aja P.M., Awuchi C.G. Antioxidant, phytochemical, and therapeutic properties of medicinal plants: A review. Int J Food Prop. 2023; 26(1): 359-388.
  19. Hirose Y., Fujita T., Ishii T., Ueno N. Antioxidative properties and flavonoid composition of Chenopodium quinoa seeds cultivated in Japan. Food Chem. 2010; 119(4): 1300-1306.
  20. Präger A., Munz S., Nkebiwe P.M., Mast B., Graeff-Hönninger S. Yield and quality characteristics of different quinoa (Chenopodium quinoa Willd.) cultivars grown under field conditions in Southwestern Germany. Agronomy. 2018; 8(10): 1-19.
  21. Maliro M.F., Guwela V.F., Nyaika J., Murphy K.M. Preliminary Studies of the Performance of Quinoa (Chenopodium quinoa Willd.) Genotypes under Irrigated and Rainfed Conditions of Central Malawi. Front Plant Sci. 2017; 8: 1-9.
  22. Souza S.P., Roos A.A., Gindri A.L., Domingues V.O., Ascari J., Guerra G.P. Neuroprotective effect of red quinoa seeds extract on scopolamine-induced declarative memory deficits in mice: The role of acetylcholinesterase and oxidative stress. J Funct Foods. 2020; 69: 1-8.
  23. Nsimba R. Y., Kikuzaki H., Konishi Y. Antioxidant activity of various extracts and fractions of Chenopodium quinoa and Amaranthus spp. seeds. Food Chem. 2008; 106(2), 760-766.Soland S. F., Laima S. K. Phenolics and cold tolerance of Brassica napus. Plant Agriculture. 1999; 1: 1-5.
  24. Kaijv M., Sheng L., Chao C. Antioxidation of flavonoids of Green Rhizome. J Food Sci. 2006; 27(3): 110-115.
  25. Oyaizu M. Studies on products of browning reaction antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr Diet. 1986; 44(6): 307-315.
  26. Wettasinghe M., Shahidi F. Oxidative stability of cooked comminuted lean pork as affected by alkali and alkali‐earth halides. J Food Sci. 1996; 61(6): 1160-1164.
  27. Guney Eskiler G., Eryilmaz I. E., Yurdacan B., Egeli U., Cecener G., Tunca B. Synergistic effects of hormone therapy drugs and usnic acid on hormone receptor‐positive breast and prostate cancer cells. J Biochem Mol Toxicol. 2019; 33(8): 1-13.
  28. Lim J.G., Park H.M., Yoon K.S. Analysis of saponin composition and comparison of the antioxidant activity of various parts of the quinoa plant (Chenopodium quinoa Willd.). Food Sci Nutr. 2020; 8(1): 694-702.
  29. Stikić R.I., Milinčić D. D., Kostić A.Ž., Jovanović Z.B., Gašić U.M., Tešić Ž.L., Pešić M.B. Polyphenolic profiles, antioxidant, and in vitro anticancer activities of the seeds of Puno and Titicaca quinoa cultivars. Cereal Chem. 2020; 97(3): 626-633.
  30. Pellegrini M., Lucas-Gonzales R., Ricci A., Fontecha J., Fernandez-Lopez J., Perez-Alvarez J. A., Viuda-Martos M. Chemical, fatty acid, polyphenolic profile, techno-functional and antioxidant properties of flours obtained from quinoa (Chenopodium quinoa Willd) seeds. Ind Crops Prod. 2018; 111: 38-46.
  31. Chen X., Zhang Y., Cao B., Wei X., Shen Z., Su N. Assessment and comparison of nutritional qualities of thirty quinoa (Chenopodium quinoa Willd.) seed varieties. Food Chem. 2023; 19: 1-9.
  32. Liu M., Zhu K., Yao Y., Chen Y., Guo H., Ren G., ... LiJ. Antioxidant, anti‐inflammatory, and antitumor activities of phenolic compounds from white, red, and black Chenopodium quinoa seed. Cereal Chem. 2020; 97(3): 703-713.
  33. Han Y., Chi J., Zhang M., Zhang R., Fan S., Huang F., Liu L. Characterization of saponins and phenolic compounds: Antioxidant activity and inhibitory effects on α-glucosidase in different varieties of colored quinoa (Chenopodium quinoa Willd). Biosci Biotech Bioch. 2019; 83(11): 2128–2139.
  34. Liu Y., Liu J., Kong Z., Huan X., Li L., Zhang P., Qin P. Transcriptomics and metabolomics analyses of the mechanism of flavonoid synthesis in seeds of differently colored quinoa strains. Genomics. 2022; 114(1): 138-148.
  35. Piñuel L., Boeri P., Zubillaga F., Barrio D.A., Torreta J., Cruz A., Carrillo W. Production of white, red and black quinoa (Chenopodium quinoa Willd Var. Real) protein isolates and its hydrolysates in germinated and non-germinated quinoa samples and antioxidant activity evaluation. Plants. 2019; 8(8): 1-18.
  36. Ong E. S., Pek C. J. N., Tan J. C. W., Leo C. H. Antioxidant and cytoprotective effect of quinoa (Chenopodium quinoa Willd.) with pressurized hot water extraction (PHWE). Antioxidants. 2020; 9(11): 1-16.
  37. Meng-meng Z.H.A.O., Xi-juan Y.A.N.G., Bin D.A.N.G., Jie Z.H.A.N.G., Wen-gang Z.H.A.N.G. Comparative analysis of nutrient quality and polyphenol composition and antioxidant activity of different colored quinoa. Food and Machinery. 2023; 36(8): 29-35.
  38. Abderrahim F., Huanatico E., Segura R., Arribas S., Gonzalez M.C., Condezo-Hoyos L. Physical features, phenolic compounds, betalains and total antioxidant capacity of coloured quinoa seeds (Chenopodium quinoa Willd.) from Peruvian Altiplano. Food Chem. 2015; 183: 83-90.
  39. Polturak G., Aharoni A. “La Vie en Rose”: biosynthesis, sources, and applications of betalain pigments. Mol Plant. 2018; 11(1): 7-22.
  40. Dang B. Analysis on phenols and antioxidant activities of quinoa resources in Qinghai. Food Ind Techol. 2019; 40(17): 30–37.
  41. Yousif Y.B., Sanaa S., Talal A., Shtywy A. Structure-activity relationships regarding the antioxidant effects of the flavonoids on human erythrocytes. J Nat Sci. 2012; 4(9): 740-747
  42. Bhaduri S. An assessment of antioxidant and anti-proliferative activities of super grain quinoa. J Food Process Technol. 2016; 7(2): 1-8.
  43. Tang Y., Li, X., Chen P.X., Zhang B., Hernandez M., Zhang H., Tsao R. Characterization of fatty acid, carotenoid, tocophenol/ tocotrienol compositions and antioxidant activities in seeds of three Chenopodium quinoa Willd. genotypes. Food Chem. 2015; 174: 502–508.
  44. Melini V., Melini F. Functional components and anti-nutritional factors in gluten-free grains: A focus on quinoa seeds. Foods. 2021; 10(2): 351.
  45. Gawlik-Dziki U., Świeca M., Sułkowski M., Dziki D., Baraniak B., Czyż J. Antioxidant and anticancer activities of Chenopodium quinoa leaves extracts–in vitro study. Food Chemical Toxicol. 2013; 57: 154-160.
Journal of Medicinal plants and By-Products

Articles in Press, Accepted Manuscript
Available Online from 10 February 2024

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