Investigating the Effect of Taraxacum officinale Extract on Wistar Rats’ Infertility Induced by Cadmium Chloride

Document Type : Research Paper

Authors

1 Faculty of veterinary medicine, Science and research branch, Islamic azad university, Tehran, Iran

2 Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

3 Department of Biology and Anatomical Sciences, School of medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

4 Department of clinical sciences, Faculty of veterinary medicine, Science and research branch, Islamic azad university, Tehran, Iran

5 Department of pathology, Faculty of veterinary medicine, Science and research branch, Islamic Azad University, Tehran, Iran

Abstract

This study was conducted to investigate the efficacy of Taraxacum officinale F.H.Wigg. extract on the infertility of male rats. In this research, we investigated the impacts of T. officinale extract on the infertile cadmium chloride-induced Wistar rats. T. officinale extract was fed to mice daily for 28 days with the help of a gavage needle. Then, all 40 rats were anesthetized with ether and dissected, then epididymis were examined morphologically. Also, we analyzed their right tests for cell line counts and H/E staining and their left testicles were analyzed to evaluate superoxide dismutase (SOD) and malondialdehyde (MDA) enzymes. Our analysis of three sperm quality parameters showed a significant increase in viability, motility and the number of sperm cells in infertile groups treated with 100, 200, and 400 mg/kg T. officinale extract. Our SOD analysis revealed T. officinale extract caused to produce more enzymes in the healthy experimental groups, even compared to the healthy control group. Also, our analysis indicated that increasing the dosage of the T. officinale extract resulted in increasing enzyme production in infertile groups. MDA analyses showed that the healthy experimental groups produced a lower amount of MDA enzyme, which is almost the same level as the healthy control group. Also, due to the usage of T. officinale extract, the level of lipid peroxidation and MDA significantly decreased. In the current study, T. officinale extract can have significant positive impacts on the viability, motility, number of sperms in oxidative stress conditions.

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References

  1. Noh S., Go A., Kim D.B., Park M., Jeon H.W., Kim B. Role of antioxidant natural products in management of infertility: a review of their medicinal potential. Antioxidants. 2020;9(10):957.
  2. Chung H.J., Noh Y., Kim M.S., Jang A., Lee C.E., Myung S.C. Steroidogenic effects of Taraxacum officinale extract on the levels of steroidogenic enzymes in mouse Leydig cells. Animal Cells and Systems. 2018;22(6):407-14.
  3. Das S., Roychoudhury S., Dey A., Jha N.K., Kumar D., Roychoudhury S., et al. Bacteriospermia and male infertility: Role of oxidative stress. Oxidative Stress and Toxicity in Reproductive Biology and Medicine: A Comprehensive Update on Male Infertility-Volume One: Springer; 2022. p. 141-63.
  4. Abdel-Magied N., Abdel Fattah S.M., Elkady A.A. Differential effect of Taraxacum officinale L.(dandelion) root extract on hepatic and testicular tissues of rats exposed to ionizing radiation. Molecular Biology Reports. 2019;46:4893-907.
  5. Sethumadhavan N. Causes, Diagnosis and Management of Male Infertility: Phytocompounds as Natural SERMs-A Review. J. Advanced Scientific Research. 2021;12(03):29-34.
  6. Akbar S., Akbar S. Taraxacum officinale (L.) Weber ex FH Wigg (Asteraceae/Compositae) (Syns.: T. dens-leonis Desf.; T. vulgare (Lam.) Schrank). Handbook of 200 Medicinal Plants: A Comprehensive Review of Their Traditional Medical Uses and Scientific Justifications. 2020:1743-51.
  7. Younis N., Mahasneh A. Probiotics and the envisaged role in treating human infertility. Middle East Fertility Society J. 2020;25:1-9.
  8. Zhou SH., Deng Y.F., Weng Z.W., Weng H.W., Liu Z.D. Traditional Chinese medicine as a remedy for male infertility: a review. The world journal of men's health. 2019;37(2):175.
  9. Rahman A.O., Purwakanthi A., Dewi H. Antifertility effect of betel nut (Areca catechu L) in male rat. Medisains. 2020;18(2):52-7.
  10. Ommati M.M., Li H., Jamshidzadeh A., Khoshghadam F., Retana-Márquez S., Lu Y., et al. The crucial role of oxidative stress in non-alcoholic fatty liver disease-induced male reproductive toxicity: the ameliorative effects of Iranian indigenous probiotics. Naunyn-Schmiedeberg's Archives of Pharmacology. 2022;395(2):247-65.
  11. Masuku N.P., Unuofin J.O., Lebelo S.L. Phytochemical content, antioxidant activities and androgenic properties of four South African medicinal plants. J. Herbmed Pharmacology. 2020;9(3):245-56.
  12. Maslova N.F., Litvinova E.V., Bomko T.V. Patent researches and the study of the effectiveness of Kamavit phytocomposition for normalizing the functions of the Reproductive system in men. Res. J. Pharmacy and Technology. 2020;13(8):3909-14.
  13. SARAÇI A., DAMO R. Semantic reflections of human/animal genitalia in Albanian plant names. Annals of the University of Craiova Series Philology Linguistics. 2021;43(1).
  14. Liang H., Zhang S., Li Z. Ginsenoside Rg3 protects mouse leydig cells against triptolide by downregulation of miR-26a. Drug Design, Development and Therapy. 2019:2057-66.
  15. Boye A., Osei-Owusu A.K., Koffuor G.A., Barku VYA, Asiamah E.A., Asante E. Assessment of polyscias frutcosa (L.) Harm (Araliaceae) leaf extract on male fertility in rats. 2018.
  16. Prabhu Y.D., Gopalakrishnan A.V., Roopan S.M. Medicinal plants and polycystic ovary syndrome. Phytopharmaceuticals: Potential Therapeutic Applications. 2021:287-300.
  17. Wollenweber T.E, van Deenen N., Roelfs K.U., Prüfer D., Gronover C.S. Microscopic and transcriptomic analysis of pollination processes in self-incompatible Taraxacum koksaghyz. Plants. 2021;10(3):555.
  18. Lans C., Taylor-Swanson L., Westfall R. Herbal fertility treatments used in North America from colonial times to 1900, and their potential for improving the success rate of assisted reproductive technology. Reproductive Biomedicine & Society Online. 2018;5:60-81.
  19. Mohammed A.Q., Amin A. Effects of supplementation cinnamon cassia and zingiber officinale powder on reproductive performance of broiler breeder male. Plant Arch. 2019;19:567.
  20. Abu-Taweel G.M. Curcumin palliative effects on sexual behavior, fertility and reproductive hormones disorders in mercuric chloride intoxicated mice offspring. J King Saud Uni Sci. 2020;32(2):1293-9.
Journal of Medicinal plants and By-Products
Volume 14, Issue 2
March and April 2025
Pages 121-126

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