A factorial field experiment based on a randomized complete block design with three replications was conducted to investigate the effect of seed priming and soil application of humic acid on the growth and yield of Roselle (Hibiscus sabdariffa L.). The research was carried out in Farm Research of Shirvan Faculty of Agriculture in 2020. Experimental factors included seed priming with three levels of humic acid (0 (P1), 2.5g/l (P2) and 5g/l (P3)) and two levels of humic acid soil application (0 (S1) and 6 liters per hectare humic acid (S2)). The results of the statistics analysis showed that the application of humic acid (both seed priming and soil application) significantly increased the growth traits and yield of Roselle plants compared to non-humic acid-treated plants (P1S1). Plants treated with P3S2 significantly had the highest plant height (167.3 cm), number of branches per plant (9.8), number of leaves per plant (81), leaf dry weight (34 g/m2), leaf area index (5.2), calyx yield (53.3 g/m2), number of seeds per plant (882), seed yield (1281 kg/ha) and biological yield (4230 kg/ha). However, non-humic acid-treated plants (P1S1) had the lowest plant height (133.5cm), number of branches per plant (6.7), number of leaves per plant (46), leaf dry weight (18.7 g/m2), leaf area index (2.9), calyx yield (37.3 g/m2), number of seeds per plant (554), seed yield (977 kg/ha) and biological yield (2900 kg/ha). The results also showed that soil application of humic acid had a more positive effect on the growth traits of Roselle plants than seed priming. However, leaf chlorophyll content and weight of thousand seeds (g) were not affected by both seed priming and soil application of humic acid.
Mohamed B.B., Sulaiman A.A., Dahab AA. Roselle (Hibiscus sabdariffa L.) in Sudan, Cultivation and Their Uses. Bull. Environ. Pharmacol. Life Sci. 2012;1(6):48 – 54.
Khalil S.E., Abdel-Kader A.S. The Influence of Soil Moisture Stress on Growth, Water Relation and Fruit Quality of Hibisicus sabdariffa L. Grown within Different Soil Types. Natu. Sci. 2011;9(4):62-74.
Sonar B.A., Kamble V.R., Chavan P.D. Native A.M., Fungal Colonization in Three Hibiscus Species under NaCl Induced Salinity. J. Pharm. Biol. Sci. 2013;5(6):7-13.
Hirunpanich V., Utaipat A., Morales N.P., Bunyapraphatsara N., Sato H., Herunsalee A. Antioxidant effects of aqueous extracts from dried calyx of Hibiscus sabdariffa Linn. (Roselle) in vitro using rat low-density lipoprotein (LDL). Biol Pharm Bull. 2005;28(3):481-484.
Olaleye Tolulope M. Cytotoxicity and antibacterial activity of methanolic extract of Hibiscus sabdariffa. J Med Plants Res. 2007;1(1):9-13.
Peng-Kong W., Yusof S., Ghazali H.M., Man Y.B. Physico-chemical characteristics of Roselle (Hibiscus sabdariffa L.). J. Nutr Food Sci. 2002; 32: 68-73.
Wong P.K, Yusof S., Ghazali H.M., Bin Che Man Y. Optimization of hot water extraction of Roselle juice using response surface methodology: a comparative study with other extraction methods. J Sci Food Agric. 2002; 83:1273-1278.
Amor B.B., Allaf K. Impact of texturing using instant pressure drop treatment prior to solvent extraction of anthocyanins from Malaysian Roselle (Hibiscus sabdariffa). Food Chem. 2009; 115:820–825.
Cisse M., Vaillant F., Kane A., Ndiaye O., Dornier M. Impact of the extraction procedure on the kinetics of anthocyanin and colour degradation of Roselle extracts during storage. J Sci Food Agric. 2011; 92: 1214-1221.
Fasoyiro S.B, Babalola S.O., Owosibo T. Chemical Composition and Sensory Quality of Fruit-Flavoured Roselle (Hibiscus sabdariffa) Drinks. World J. of Agricultural Sciences. 2005;1(2):161-164.
Mohd-Esa N., Hern F.S., Ismail A., Yee C.L. Antioxidant Activity in Different Parts of Roselle (Hibiscus sabdariffa L.) Extracts and Potential Exploitation of the Seeds. Food Chem. 2010;122:1055-1060.
Sabzevari S., Khazaie H.R., Kafi M. Effect of humic acid on root and shoot growth of two wheat cultivars (Triticum aestivum L.). J. Water Soil. 2009; 23: 87-94. (In Persian).
Cangi R., Tarakcioglu C., Yasar H. Effect of humic acid applications on yield, fruit characteristic and nutrient uptake in Ercis grape (V. vinifera L.) cultivars Asian Journal of Chemistry. 2006;18:1493-1499.
Muscolo A., Sidari M., Francioso O., Tugnoli V., Nardi S. The auxin-like activity of humic substances is related to membrane interactions in carrot cell culture. J. of Chemical Ecology. 2007;33:115-129.
Canellas L.P., Dobbss L. B., Oliveira A.L., Chagas J.G., Aguiar N. O., Rumjanek V. M., Novotny E. H., Olivares F. L., Spaccini R., Piccolo A. Chemical properties of humic matter as related to induction of plant lateral roots. European Journal of Soil Science. 2012;63:315–324.
Bakry B.A., Taha M.H., Abdelgawad Z.A., Abdallah M.M.S. The role of humic acid and proline on growth, chemical constituents and yield quantity and quality of three flax cultivars grown under saline soil conditions. Agric. Sci. 2014;5:1566-1575.
Rose M.T., Patti A.F., Little K.R., Brown A.L., Jackson W.R., Cavagnaro T.R. A Meta-Analysis and Review of Plant-Growth Response to Humic Substances: Practical Implications for Agric. Advan. Agron. 2014; 24: 37- 89.
Koocheki A., Fallahi H.R., Amiri M.B., Ehyaei H.R. Effects of humic acid application and mother corm weight on yield and growth of saffron (Crocus sativus L.). J. Agroecol. 2016; 7(4): 1-18.
Khalil S.E., Abdel-Kader A.S. The Influence of Soil Moisture Stress on Growth, Water Relation and Fruit Quality of Hibisicus sabdariffa L. Grown within Different Soil Types. Natu. Sci. 2011; 9(4): 62-74.
Bettoni M.M., Mogor A. F., Pauletti V., Goicoechea N. Growth and metabolism of onion seedlings as affected by the application of humic substances, mycorrhizal inoculation and elevated CO2. Sci. Horti. 2014; 180: 227-235.
Garcia M.C.V., Estrella F.S., Lopes M.J., Moreno J. Influence of compost amendment on soil biological properties and plants. Dynamic soil Dynamic Plant. 2008; 1:1-9.
Sanjari-Mijani M. 2014. Effect of Humic Acid and Drought Stress on Qualitative and Quantitative Indices of Roselle (Hibiscus sabdariffa) Medicinal Plant. Univ. of Zabol, M.Sc. Thesis.
Sanjari-Mijani M., Sirousmehr A., Fakheri B.A. The Effects of Drought Stress and Humic Acid on Some Physiological Characteristics of Roselle (Hibiscus sabdariffa). Agric. Crop. Manag. 2015; 17(2): 403-414.
Zhang X., Zhou D., Ervin E. H., Evanylo G. Cataldi, D., & Li,J. Bio solids impact on antioxidant metabolism associated with drought tolerance in tall fescue. Hortscience, 2012;47:1550–1555
Tavassoli A. Effect of Seed Priming on Yield and Some Qualitative Indicators of Roselle (Hibiscus sabdariffa L.) under Drought Stress. Desert Management: 2021;9(1): 81-96.
Ebrahimi M., Miri E. Effect of Humic Acid on Seed Germination and Seedling Growth of Borago officinalis and Cichorium intybus. Ecopercia. 2016;4(1):1239-1249.
Karami L., Hedayat M., Farahbakhsh S. Effect of salicylic acid priming on seed germination and morphophysiological and biochemical characteristics of tomato seedling (Lycopersicom esculentun). Iranian Journal of Seed Research. 2020;7(1):165-180.
Jones C.A., Jacobsen J.S., Mugaas A. Effects of humic acid on phosphorus availability and spring wheat yield. J. Facts Ferilizer. 2004; 32-41.
Hanafy Ahmad A.H., Nesiem M.R., Hewedy A.M. Sallam H.E.I.S. Effect of some simulative compounds on growth, yield and chemical composition of snap bean plants grown under calcareous soil conditions J. of Amer. Science. 2010; 6(10):552-569.
Albayrak S., Camas N. Effect of different levels and application times of humic acid on root and leaf yield and yield component of forage Turpin. Journal of Agronomy. 2005;42:130-133.
Dadkhah, A. (2024). Effect of Seed Priming and Soil Application of Humic Acid on Growth and Yield of Roselle (Hibiscus sabdariffa L.). Journal of Medicinal plants and By-Products, 13(3), 513-518. doi: 10.22034/jmpb.2024.362229.1556
MLA
Alireza Dadkhah. "Effect of Seed Priming and Soil Application of Humic Acid on Growth and Yield of Roselle (Hibiscus sabdariffa L.)". Journal of Medicinal plants and By-Products, 13, 3, 2024, 513-518. doi: 10.22034/jmpb.2024.362229.1556
HARVARD
Dadkhah, A. (2024). 'Effect of Seed Priming and Soil Application of Humic Acid on Growth and Yield of Roselle (Hibiscus sabdariffa L.)', Journal of Medicinal plants and By-Products, 13(3), pp. 513-518. doi: 10.22034/jmpb.2024.362229.1556
VANCOUVER
Dadkhah, A. Effect of Seed Priming and Soil Application of Humic Acid on Growth and Yield of Roselle (Hibiscus sabdariffa L.). Journal of Medicinal plants and By-Products, 2024; 13(3): 513-518. doi: 10.22034/jmpb.2024.362229.1556