Document Type : Research Paper
Authors
1
Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia
2
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia
3
Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia
4
Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia
5
National Institute of Fundamental Studies Hanthana Road, 20000 Kandy, Sri Lanka
Abstract
Background: The abundance of plant resources in Malaysia has gained the attention of many researchers to explore more pharmacological effects of plant’s essential oil (EO). EO from Stevia rebaudiana Bertoni (SrB-EO) leaves consists of a rich blend of a variety of volatile molecules and bioactive compounds which currently attract the attention of a lot of researchers due to its high potential to play a role in the therapeutic activity. Methods: In the present study, SrB-EO used was extracted using a Soxhlet extractor in absolute ethanol. The cell viability on SrB-EO has been investigated using Hs27 skin fibroblast cells against two biological assessments; anti-inflammatory and antimicrobial assays. Results: It shows that 500 µg/mL was the highest concentration of SrB-EO which is considered non-toxic (82.08% cell viability) and demonstrates a primary significant cell proliferative effect toward cells. The maximum inhibition percentage of nitrite of SrB-EO was 33.12%, which showed a good anti-inflammatory property and SrB-EO showed an antimicrobial effect toward Gram-positive bacteria; Bacillus subtilis B29 and Staphylococcus aureus ATCC 43300. The minimal inhibition concentration (MIC) of B. subtilis and S. aureus were 6.25 mg/mL and 3.13 mg/mL of SrB-EO respectively, and the minimal bactericidal concentration (MBC) of B. subtilis and S. aureus were 12.50 mg/mL and 10.42 mg/mL of SrB-EO, respectively. Conclusion: This study concluded that a low concentration of SrB-EO is non-toxic toward human skin cells and these EOs possess satisfactory anti-inflammatory and antibacterial properties. Thus, SrB-EO can be used as an alternative therapeutic agent for topical application in the medicinal field.
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