Influence of Vermicompost and Nitrogen Treatments on the Enhancement of Morphophysiological Traits and Essential Oil Composition of Spearmint (Mentha spicata L.) for Double Harvesting Seasons

Document Type : Research Paper

Authors

Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran

Abstract

This research investigates the impact of organic and chemical fertilizers on the total production and morphophysiological characteristics of Mentha spicata L., a medicinal plant, during two growing seasons in 2021. The study was conducted in a randomized complete block design at Zanjan University's research farm, employing treatments of vermicompost (at rates of 5, 10, and 15 tons per hectare), urea (at rates of 50, 100, and 150 kilograms per hectare), and a control treatment. Significant differences were observed in all measured indices between the fertilizer treatments and harvest times. Particularly, the application of vermicompost at a concentration of 10 tons per hectare significantly enhanced fresh and dry weights, stem height and diameter, number of branches, percentage and yield of essential oils, chlorophyll a, total chlorophyll, carotenoids, total phenols, flavonoids, and antioxidant capacity compared to other treatments. The urea treatment also significantly increased chlorophyll b, leaf width, and length compared to other treatments. Enzyme measurements conducted during one season revealed the highest levels of the APX enzyme in the vermicompost treatment (15 tons per hectare), the CAT enzyme in the vermicompost treatments (10 and 15 tons per hectare), and the SOD enzyme in the vermicompost treatment (10 tons per hectare) during the second harvest. Conversely, the control treatment exhibited the lowest levels across all measured traits. Overall, the results highlight the beneficial effects of using organic and environmentally friendly fertilizers in improving essential oils content, physiological traits, and the overall yield of M. spicata L., suggesting their potential for enhancing sustainable agriculture practices and medicinal plant productivity.

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Main Subjects

References

  1. Meskelu E., Mohammed M., Yimenu F., Derese Y. Spearmint Mentha spicata L. response to deficit irrigation. Int. J. Rec. Res. Life Sci. 2014.1: 22-30.
  2. Brahmi F., Khodir M., Mohamed C., Pierre D. Chemical composition and biological activities of Mentha species. Aromatic and Med Plants-Back to Nature. 2017;10: 47-79.
  3. Shahbazi Y. Chemical composition and in vitro antibacterial activity of Mentha spicata essential oil against common food-borne pathogenic bacteria. J. Pathogens. 2015.
  4. Ducasse V., Capowiez Y., Peigné J. Vermicomposting of municipal solid waste as a possible lever for the development of sustainable agriculture. A review. Agronomy for Sustainable Development. 2022;42(5): 1-17.
  5. Tariyal Y.S., Ansari S., Prasad P. Organic Cultivation of Medicinal and Aromatic Plants: a Review. J. Stress Physiology & Biochem. 2021;17(1): 129-133.
  6. Nene Y.L. A critical discussion on the methods currently recommended to support organic crop farming in India. Asian Agri-History. 2017;21(3): 267-285.
  7. Benti G., Tadesse F., Degefa G., Jafar M., Biri A. Integrated Effects of Vermicompost and Nitrogen on Yield and Yield Components of Tomato Lycopersicum esculentum L. in Lowlands of Eastern Harerghe. Plant. 2021;9(3): 81.
  8. Raza S.T., Wu J., Rene E.R., Ali Z., Chen Z. Reuse of agricultural wastes, manure, and biochar as an organic amendment: A review on its implications for vermicomposting technology. Journal of Cleaner Production. 2022;132200.
  9. Makkar C., Singh J., Parkash C., Singh S., Vig A.P., Dhaliwal S.S. Vermicompost acts as bio-modulator for plants under stress and non-stress conditions. Environment, Development and Sustainability. 2022;1-52.
  10. Sharma T., Kaur A., Saajan S., Thakur R. Effect of nitrogen on growth and yield of medicinal plants. A review paper. Eur. J. Mol. Clin. Med. 2020;7: 2771-2776.
  11. Eghlima G., Kheiry A., Sanikhani M., Hadian J., Aelaei M., Nejad Ebrahimi S. Investigation of Phytochemical Variability, Antioxidant Activity and Ecological Conditions of Native Iranian Glycyrrhiza glabra L. Int. J. Horticultural Sci. Technol. 2022;7(4): 387-400.
  12. Alhasan A.S., Khadim A.A., Al-Ameri D.T., Saleh O.S., Al-Alwash F.H.J., Kadkim H.R. Effect of Applying Different Levels of Nitrogen Fertilizer on Growth and Essential Oil of Chamomile (Matricaria chamomilla L.). In IOP Conference Series: Earth and Environ. Sci. 2022;(Vol. 1060, No. 1, p. 012102). IOP Publishing.
  13. Boliko M.C. FAO and the situation of food security and nutrition in the world. J. Nutritional Sci. Vitaminology. 2019;65(Supplement): S4-S8.
  14. Kumar A., Gupta R.K. The effects of vermicompost on growth and yield parameters of vegetable crop radish Raphanus sativus. J. Pharmacognosy and Phytochemistry. 2018;7(2): 589-592.
  15. Zahraa L.S.J.A.T., Al-Budairy J.K. The Effect of Vermicompost, Urea, and Seaweed Extracts on Growth and Yield Characteristics of Yellow Maize (Zea mays L.). J. Al-Muthanna for Agric. Sci. 2021;8(4).
  16. Kalinova J., Vrchotova N. The influence of organic and conventional crop management, variety and year on the yield and flavonoid level in common buckwheat groats. Food Chem. 2011;127(2). 602-608.
  17. Keshavarz Mirzamohammadi H., Modarres-Sanavy S.A.M., Sefidkon F., Mokhtassi-Bidgoli A., Mirjalili M.H. Irrigation and fertilizer treatments affecting rosmarinic acid accumulation, total phenolic content, antioxidant potential and correlation between them in peppermint (Mentha piperita L.). Irrigation Sci. 2021;39(5): 671-683.
  18. Chang C.C., Yang M.H., Wen H.M., Chern J.C. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food and Drug Analysis. 2002;10(3).
  19. Dehghan G., Khoshkam Z. Tin (II)–quercetin complex: Synthesis, spectral characterisation and antioxidant activity. Food Chem. 2012;131(2): 422-426.
  20. Nasr F., Razavi F., Rabiei V., Gohari G., Ali S., Hano C. Attenuation of Chilling Injury and Improving Antioxidant Capacity of Persimmon Fruit by Arginine Application. Foods. 2022;11(16): 2419.
  21. Hosseinzadeh S.R., Amiri H., Ismaili A. Evaluation of photosynthesis, physiological, and biochemical responses of chickpea Cicer arietinum L. cv. Pirouz under water deficit stress and use of vermicompost fertilizer. J. Integrative Agric. 2018;17(11): 2426-2437.
  22. Dinani E.T., Asghari H.R., Gholami A., Masoumi A. Replacement of vermicompost for nitrogen fertilizer as a source of nitrogen on two cultivars of coriander Coriandrum sativum. Acta Horticulturae. 2014;(1018): 343-349.
  23. Bloom A.J. The increasing importance of distinguishing among plant nitrogen sources. Current Opinion in Plant Biology. 2015;25: 10-16.
  24. Muñoz-Huerta R.F., Guevara-Gonzalez R.G., Contreras-Medina L.M., Torres-Pacheco I., Prado-Olivarez J., Ocampo-Velazquez R.V. A review of methods for sensing the nitrogen status in plants: advantages, disadvantages and recent advances. Sensors. 2013;13(8): 10823-10843.
  25. Kalinova J., Vrchotova N. The influence of organic and conventional crop management, variety and year on the yield and flavonoid level in common buckwheat groats. Food Chem. 2011;127(2). 602-608.
  26. Afkari A. An investigation to the vermicompost efficacy on the activity level of antioxidant enzymes and photosynthetic pigments of borage Borago officinalis L. under salinity stress conditions. Russian Agric. Sci. 2018;44(4): 310-317.
  27. Salehi A., Gholamhoseini M., Ataei R., Sefikon F., Ghalavand A. Effects of zeolite, bio-and organic fertilizers application on german chamomile yield and essential oil composition. J. Essential Oil Bearing Plants. 2018;21(1): 116-130.
  28. Joshi R., Singh J., Vig A.P. Vermicompost as an effective organic fertilizer and biocontrol agent: effect on growth, yield and quality of plants. Reviews in Environ. Sci. Biotechnology. 2015;14(1): 137-159.
  29. Rezvani M.P., Amin G.A., Bakhshaie S., Jafari L. The Effect of Organic and Biofertilizers on some Quantitative Characteristics and Essential Oil Content of Summer Savory (Satureja hortensis L.). 2013.
  30. Baldotto L.E., Baldotto M.A. Adventitious rooting on the Brazilian red-cloak and sanchezia after application of indole-butyric and humic acids. Horticultura Brasileira. 2014;32: 434-439.
Journal of Medicinal plants and By-Products
Volume 13, Issue 4
December 2024
Pages 1016-1025

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