The Effect of Biosynthesized Zinc Oxide Nanoparticles Capped by Scenedesmus obliquus on Apoptosis and Gene Expression of p53 and VEGF in Breast Cancer Cell Line

Document Type : Research Paper

Authors

Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

Abstract

Breast cancer is the most prevalent form of cancer in women. This condition poses a significant public health concern that requires further research to understand its prognosis better and proper treatment options. Scenedesmus obiquus algae has unique biological compounds that can be effective in the synthesis of nanoparticles. The objective of this study is to examine how the impact of ZnO NPs, which are produced by the S. obliquus algae, affects cell death and gene expression in a breast cancer cell line. MDA-MB-231 cells are a subtype of breast cancer cells known for their high aggressiveness and connection to the triple-negative breast cancer subtype. The impact of biosynthesized ZnO NPs (0, 800, 1600, and 2400 μg/ml) on apoptosis of the MDA-MB-231 cell line and VEGF and p53 gene expression in breast cancer cells was assessed. The absorption band at 362 nm exhibited an absorption maximum that confirmed the synthesis of ZnO NPs. Zinc oxide nanoparticles exhibited significant toxicity towards MDA-MB-231 cell lines, with an IC50 value of 1050 μg/ml. The findings indicated that 1600 μg/ml of ZnO NPs had the most potent inhibitory effect on the growth (71.1%) of MDA-MB-231cultures. The ZnO NPs increased the expression of p53 and reduced the expression of VEGF. This study demonstrates that nanoparticles created using S. obliquus algae, which possess anti-cancer properties, have potential effectiveness in treating cancer. However, it is essential to conduct appropriate in vivo studies to investigate its impact on additional biochemical indicators.

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References

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Journal of Medicinal plants and By-Products
Volume 14, Issue 2
March and April 2025
Pages 149-155

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