Comparative Study of the Antioxidant, Antibacterial, Antidiabetic Analysis and Chemical Compounds of Two Different Geographical Regions Propolis for Production of Functional Chewing Gum

Document Type : Research Paper

Authors

1 Nutrition and Food Sciences Research Center, Medical Sciences Tehran, Islamic Azad University, Tehran, Iran

2 Department of Food Science & Technology, Safadasht Branch, Islamic Azad University, Tehran, Iran

3 Department of Food Science and Engineering, University College of Agriculture & Natural, Resources, University of Tehran, Tehran, Iran

Abstract

Apis mellifera L. is a honeybee species responsible for gathering propolis, a resinous material from tree cracks. This study assessed the antibacterial activity by agar well diffusion, antioxidant activity by Trolox equivalent antioxidant capacity assay (TEAC), and α-glucosidase inhibitory activity of north (Mazandaran) and south (Khuzestan) propolis of Iran. The ethanolic extract of propolis (EEP) of both samples demonstrates the highest antibacterial activity against gram-positive bacteria Streptococcus mutans, Streptococcus sanguinis, and Khuzestan EEP was more effective against assessed bacteria than that of Mazandaran. The results of antioxidant activity indicated that higher EEP concentration had more antioxidant activity (31.2 mg/ml of Khuzestan EEP and Mazandaran EEP showed 16.71±0.05 µmol/L and 16.35±0.19 µmol/L Trolox antioxidant activity, respectively). Both EEPs inhibited the α-glucosidase enzyme. EEP chemical compounds have been investigated by GC-MS. Khuzestan and Mazandaran EEP demonstrated 49 and 29 volatile compounds. Chewing gums were prepared with different Khuzestan propolis concentrations, and sensory evaluation of basic properties (smell, texture, appearance, taste, bubble size) was carried out, with results demonstrating that Khuzestan propolis can be used as a functional food and can be formulated into chewing gums as an antibacterial agent.

Keywords

Main Subjects

References

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Journal of Medicinal plants and By-Products
Volume 13, Special
November 2024
Pages 791-805

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