Effects of Persian Shallot Extract on Inhibition of Adipogenesis in 3T3-L1 Cells

Document Type : Research Paper

Authors

1 Department of Medical Biotechnology, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

2 Department of Medical Basic Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

3 Department of Biotechnology, School of Pharmacy, Alborz university of Medical Sciences, Karaj, Iran

Abstract

Today, the industrial world has encountered the problem of obesity which can be a risk factor for diseases such as cancer, cardiovascular diseases, and type 2 diabetes. Various strategies have been proposed for the management of obesity, one of which includes the utilization of medicinal plants. This study examines the impact of hydroalcoholic extract from Persian shallots on the adipogenesis of the 3T3-L1 cell line. The cell viability of Persian shallot extract was evaluated using the MTT assay following treatment with various concentrations of the extract, with assessments conducted at 24-hour and 48-hour intervals. The lipid content of the cells was evaluated through Oil-Red O staining, in conjunction with quantitative real-time PCR analysis to assess the expression of the PPARγ gene. The results from the MTT test indicated that concentrations below 250 μg/ml maintain cell viability levels exceeding 70%. However, the concentration of 125 μg/ml was also employed in this context. Upon reaching 80% confluency, the cells are treated with a differentiation medium for two days. The subsequent step involved transitioning to an expansion medium for six days. The expansion medium was refreshed every 48 hours during this period. During the differentiation process on Day 8, we observed the presence of lipid droplets that had accumulated. In the Oil-Red O staining analysis, our observations indicated a significant reduction in the lipid content of mature adipocytes treated with 125 μg/ml compared to the control group of mature adipocytes. The gene expression analysis revealed a significant decrease in PPARγ expression on the eighth day of differentiation, with a reduction of 77% compared to the control group (P value = 0.028). These findings indicate that Persian shallot may represent a viable treatment option for managing obesity.

Keywords

Main Subjects

Journal of Medicinal plants and By-Products

Articles in Press, Accepted Manuscript
Available Online from 26 October 2024

Files

Share

How to cite

Statistics