Effects of Persian Shallot Extract on Inhibition of Adipogenesis in 3T3-L1 Cells

Document Type : Research Paper

Authors

1 Department of Medical Biotechnology, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

2 Department of Medical Basic Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

3 Department of Biotechnology, School of Pharmacy, Alborz University of Medical Sciences, Karaj, Iran

Abstract

Today, the industrial world has encountered the problem of obesity which can be a risk factor for diseases such as cancer, cardiovascular diseases, and type 2 diabetes. Various strategies have been proposed for the management of obesity, one of which includes the utilization of medicinal plants. This study examines the impact of hydroalcoholic extract from Persian shallots on the adipogenesis of the 3T3-L1 cell line. The cell viability of Persian shallot extract was evaluated using the MTT assay following treatment with various concentrations of the extract, with assessments conducted at 24-hour and 48-hour intervals. The lipid content of the cells was evaluated through Oil-Red O staining, in conjunction with quantitative real-time PCR analysis to assess the expression of the PPARγ gene. The results from the MTT test indicated that concentrations below 250 μg/ml maintain cell viability levels exceeding 70%. However, the concentration of 125 μg/ml was also employed in this context. Upon reaching 80% confluency, the cells are treated with a differentiation medium for two days. The subsequent step involved transitioning to an expansion medium for six days. The expansion medium was refreshed every 48 hours during this period. During the differentiation process on Day 8, we observed the presence of lipid droplets that had accumulated. In the Oil-Red O staining analysis, our observations indicated a significant reduction in the lipid content of mature adipocytes treated with 125 μg/ml compared to the control group of mature adipocytes. The gene expression analysis revealed a significant decrease in PPARγ expression on the eighth day of differentiation, with a reduction of 77% compared to the control group (P value = 0.028). These findings indicate that Persian shallot may represent a viable treatment option for managing obesity.

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References

  1. Diels S., Vanden Berghe W., Van Hul W. Insights into the multifactorial causation of obesity by integrated genetic and epigenetic analysis. Obes. Rev. 2020; 21(7):e13019.
  2. World Health Organization. More than 1.9 billion adults were overweight in 2016, and 650 million obese. Obesity 2021 [cited 27 April 2023].
  3. Obesity Rates In The World 2023. Obesity Rates In The World 2023 [cited 27 APRIL 2023].
  4. Kushner R.F. Weight loss strategies for treatment of obesity. Prog Cardiovasc Dis. 2014; 56(4):465-472.
  5. Audano M., Pedretti S., Caruso D., Crestani M., De Fabiani E., Mitro N. Regulatory mechanisms of the early phase of white adipocyte differentiation: an overview. Cell Mol. Life Sci. 2022; 79(3): 139.
  6. Lefterova M.I., Haakonsson A.K., Lazar M.A. Mandrup S. PPARγ and the global map of adipogenesis and beyond. Trends Endocrinol. Metab. 2014. 25(6): 293-302.
  7. Salma N., Xiao H., Imbalzano A.N. Temporal recruitment of CCAAT/enhancer-binding proteins to early and late adipogenic promoters in vivo. J. Mol. Endocrinol. 2006; 36(1): 139-152.
  8. Feng S., Reuss L., Wang Y. Potential of natural products in the inhibition of adipogenesis through regulation of PPARγ expression and/or its transcriptional activity. Molecules 2016; 21(10): 1278.
  9. Kazemi S., Asgary S., Moshtaghian J., Rafieian M., Adelnia A., Shamsi F. Liver-protective effects of hydroalcoholic extract of Allium hirtifolium Boiss. in rats with alloxan-induced diabetes mellitus. ARYA Atheroscler. 2010; 6(1): 11-15.
  10. Sun, W., Shahrajabian M.H., Cheng Q. The insight and survey on medicinal properties and nutritive components of shallot. J. Med. Plant Res. 2019; 13(18): 452-457.
  11. Shahrajabian, M.H., Wenli S., Cheng Q. Chinese onion, and shallot, originated in Asia, medicinal plants for healthy daily recipes. Notulae Scientia Biologicae. 2020; 12(2): 197-207.
  12. Moldovan C., Frumuzachi O., Babotă M., Barros L., Mocan A., Carradori S., Crişan G. Therapeutic uses and pharmacological properties of Shallot (Allium ascalonicum): A systematic review. Front. Nutr. 2022; 28(9):903686.
  13. Hosseini F.S., Falahati-Pour S.K., Hajizadeh M.R., Khoshdel A., Mirzaei, M.R. Ahmadirad H. Behroozi R., Jafari N., Mahmoodi, M. Persian shallot, Allium hirtifolium Boiss, induced apoptosis in human hepatocellular carcinoma cells. Cytotechnology 2017; 69(4): 551-563
  14. Mohammadi-Motlagh H.-R., Mostafaie A., Mansouri K. Anticancer and anti-inflammatory activities of shallot (Allium ascalonicum) extract. Arch. Med. Res. 2011; 7(1): 38-44.
  15. Hosseini J., Hosseini-zijoud S.M., Oubari F., Mahmoodi M., Abbasi Oshaghi E., Rajabi Gilan N., Ghasemi S. R., Hashemi B. Hepatoprotective effects of hydroalcoholic extract of Allium hirtifolium (Persian shallot) in diabetic rats. J. Basic Clin. Physiol. Pharmacol. 2012; 23(2): 83-87
  16. Charoenchai L., Luprasong C., Meksuriyen D. Characterization of some organosulfur compounds in shallot bulbs. Thai J. Pharmaceut. Sci. (TJPS). 2018; 42.
  17. Rao V.R.B.V., Candasamy M., Bhattamisra S.K. Obesity an overview: Genetic conditions and recent developments in therapeutic interventions. Diabetes Metab. Syndr. Clin. Res. Rev. 2019; 13(3): 2112-2120.
  18. Magkos F., Hjorth M.F., Astrup A. Diet and exercise in the prevention and treatment of type 2 diabetes mellitus. Nat. Rev. Endocrinol. 2020; 16(10): 545-555.
  19. Lega I.C., Lipscombe L.L. Diabetes, obesity, and cancer—pathophysiology and clinical implications. Endocr. Rev. 2020; 41(1): 33-52.
  20. Ambele M.A., Dhanraj P., Giles R., Pepper M. S. Adipogenesis: A complex interplay of multiple molecular determinants and pathways. Int. J. Mol. Sci. 2020; 21(12):4283.
  21. Jakab J., Miškić B., Mikšić Š., Juranić B., Ćosić V., Schwarz D., Včev A. Adipogenesis as a potential anti-obesity target: A review of pharmacological treatment and natural products. Diab. Metab. Syndr. Obes: Targets and Therapy 2021; 14: 67-83.
  22. Yun J.W. Possible anti-obesity therapeutics from nature–A review. Phytochemistry 2010; 71(14-15): 1625-1641.
  23. Müller T.D., Blüher M., Tschöp M.H., DiMarchi R.D. Anti-obesity drug discovery: advances and challenges. Nat. Rev. Drug Discov. 2022; 21: 201-223.
  24. Shaik Mohamed Sayed U.F., Moshawih S., Goh H.P., Kifli N., Gupta G., Singh S.K., Chellappan D.K., Dua K., Hermansyah A., Ser H.L., et al. Natural products as novel anti-obesity agents: insights into mechanisms of action and potential for therapeutic management. Front. Pharmacol. 2023; 20(14): 1182937.
  25. Bhatwalkar S.B., Mondal R., Krishna S.B.N., Adam J.K., Govender P., Anupam R. Antibacterial Properties of Organosulfur Compounds of Garlic (Allium sativum). Front. Microbiol. 2021; 27(12): 613077.
  26. Omidifar N., Nili-Ahmadabadi A., Gholami A., Dastan D., Ahmadimoghaddam, D., Nili-Ahmadabadi H. Biochemical and histological evidence on the protective effects of Allium hirtifolium Boiss (Persian Shallot) as an herbal supplement in cadmium-induced hepatotoxicity. Evid. Based Complement. Alternat. Med. 2020; 7457504.
  27. Moradi Y., Moradi-Sardareh H., Ghasemi H., Mohammadi N., Moradi M.N., Hosseini-Zijoud S.M. Medicinal properties of Persian Shallot. Eur. J. Exp. Biol. 2013; 3(1): 371-379.
  28. Moon Y.-S. Molecular Regulation of Adipocyte Differentiation. Proceedings of the Korean Nutrition Society Conference. 2004. The Korean Nutrition Society.
  29. Tamori Y., Masugi J., Nishino N., Kasuga M. Role of peroxisome proliferator-activated receptor-γ in maintenance of the characteristics of mature 3T3-L1 adipocytes. Diabetes 2002; 51(7): 2045-2055.
  30. Farmer S. Regulation of PPARγ activity during adipogenesis. Int. J. Obes. 2005; 29(1): S13-S16.
  31. Soorni A., Akrami A.M., Abolghasemi R., Vahedi M. Transcriptome and phytochemical analyses provide insights into the organic sulfur pathway in Allium hirtifolium. Sci. Rep. 2021; 11(768).
  32. Dwivedi A., Singh S., Kumar, S., Mittal P.C. Organosulfur phytochemicals against metabolic and neurodegenerative diseases: benefits and risks. In: Phytochemicals as Lead Compounds for New Drug Discovery (Egbuna C., EDs.) 2020; Elsevier, pp. 179-194.
Journal of Medicinal plants and By-Products
Volume 14, Issue 1
January and February 2025
Pages 80-86

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