Antibiotic Resistance Modulatory Activities of Mentha Cordifolia Opiz and Mentha Arvensis L. buffered Leaves Crude Extracts against Methicillin-resistant Staphylococcus Aureus Phenotypes

Document Type : Short Communication

Authors

1 College of Medical and Biological Sciences, University of the Immaculate Conception, Davao City, Philippines

2 Graduate School Department, University of the Immaculate Conception, Davao City, Philippines

3 Clinical Laboratory and Training Center, University of the Immaculate Conception, Davao City, Philippines

Abstract

Methicillin-resistant strains of Staphylococcus aureus strains pose a critical challenge to healthcare, necessitating the development of novel therapeutic approaches. This study investigated the potential of secondary metabolites from Mentha cordifolia Opiz and Mentha arvensis L. to modulate antibiotic resistance in methicillin-resistant S. aureus clinical isolates. A rapid p-iodonitrotetrazolium chloride (INT) colorimetric assay was employed to evaluate the antibacterial activity, antibiotic resistance-modulating activities, and effects of Mentha cordifolia Opiz and Mentha arvensis L. extracts. This study focused on the interaction between these extracts and oxacillin, an antibiotic that typically exhibits high minimum inhibitory concentrations (MICs) against methicillin-resistant Staphylococcus aureus. Remarkably, the addition of the buffered crude extracts of Mentha cordifolia and Mentha arvensis to oxacillin resulted in significant modulatory activity with a modulatory factor of 2 to 413.3 at a concentration of 125 µg/mL. This modulation was observed as a decrease in oxacillin MIC against the tested methicillin-resistant S. aureus strains. Moreover, both Mentha cordifolia and Mentha arvensis demonstrated potent modulatory effects, accounting for 71% of all tested methicillin-resistant phenotypes. The results of this study open new avenues for combating the growing threat of antibiotic-resistant bacterial infections in the healthcare setting. By potentially enhancing the efficacy of existing antibiotics, such as oxacillin, this approach could lead to improved treatment outcomes for patients with methicillin-resistant S. aureus infections.

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References

  1. Baym M., Stone L.K., Kishony R. Multidrug evolutionary strategies to reverse antibiotic resistance. Sci. 2016;351.
  2. Sharma A., Gupta V.K., Pathania R. Efflux pump inhibitors for bacterial pathogens: From bench to bedside. Indian J Med Res. 2019;149.
  3. Schindler B.D., Jacinto P., Kaatz G.W. Inhibition of drug efflux pumps in Staphylococcus aureus: Current status of potentiating existing antibiotics. Future Microbiol. 2013;8.
  4. Patil S.S., et al. Prevalence of Methicillin-resistant Staphylococcus Aureus in India: A Systematic Review and Meta-analysis. Oman Med J. 2022;37.
  5. Hassanzadeh S., Ganjloo S., Pourmand M.R., Mashhadi R., Ghazvini K. Epidemiology of efflux pumps genes mediating resistance among Staphylococcus aureus; A systematic review. Microb Pathog. 2020;139.
  6. Costa S.S., et al. Genetic diversity of norA, coding for a main efflux pump of Staphylococcus aureus. Front Genet. 2019;10.
  7. Marquez B. Bacterial efflux systems and efflux pumps inhibitors. Biochimie. 2005;87.
  8. Vaou N., Stavropoulou E., Voidarou C., Tsigalou C., Bezirtzoglou E. Towards advances in medicinal plant antimicrobial activity: A review study on challenges and future perspectives. Microorganisms. 2021;9.
  9. Nayim P, Mbaveng A.T., Wamba B.E.N., Fankam A.G., Dzotam J.K., Kuete V. Antibacterial and Antibiotic-Potentiating Activities of Thirteen Cameroonian Edible Plants against Gram-Negative Resistant Phenotypes. ScientificWorldJournal. 2018;2018.
  10. AlMatar M., Albarri O., Makky E.A., Köksal F. Efflux pump inhibitors: new updates. Pharmacol Rep. 2021;73.
  11. Wamba B.E.N., Mbaveng A.T., Nayim P., Dzotam J.K., Ngalani O.J.T., Kuete V. Antistaphylococcal and antibiotic resistance modulatory activities of thirteen Cameroonian edible plants against resistant phenotypes. Int J Microbiol. 2018;2018.
  12. Muntean D., et al. Evaluation of essential oil obtained from Mentha×piperita L. against multidrug-resistant strains. Infect Drug Resist. 2019;12.
  13. Rosato A., et al. Elucidation of the synergistic action of Mentha Piperita essential oil with common antimicrobials. PLoS One. 2018;13.
  14. Hinay A.A., Sarol L.D.C. Screening of Mentha cordifolia Opiz (Yerba Buena) buffer crude extract for aspartyl protease pepsin inhibitory activity. Int J of Res in Pharmacology & Pharmacotherapeutics. 2014;3.
  15. Fankam A.G., Kuiate J.R., Kuete V. Antibacterial and antibiotic resistance modulatory activities of leaves and bark extracts of Recinodindron heudelotii (Euphorbiaceae) against multidrug-resistant Gram-negative bacteria. BMC Complement Altern Med. 2017;17.
  16. Kovač J., Gavarić N., Bucar F., Možina S.S. Antimicrobial and resistance modulatory activity of alpinia katsumadai seed phenolic extract, essential oil and post-distillation extract. Food Technol Biotechnol. 2014;52.
  17. Park Y.J., et al. Composition of volatile compounds and in vitro antimicrobial activity of nine Mentha spp. Springerplus. 2016;5.
  18. Pichersky E., Noel J.P., Dudareva N. Biosynthesis of plant volatiles: Nature’s diversity and ingenuity. Science. 2006;311.
  19. Sanhueza L., Melo R., Montero R., Maisey K., Mendoza L., Wilkens M. Synergistic interactions between phenolic compounds identified in grape pomace extract with antibiotics of different classes against Staphylococcus aureus and Escherichia coli. PLoS One. 2017;12.
  20. Mancuso M. The Antibacterial Activity of Mentha. In: Herbs and Spices. 2020.
  21. Mahmoudi H., Shokoohizadeh L., Zare Fahim N., Mohamadi Bardebari A., Moradkhani S., Alikhani M.Y. Detection of adeABC efllux pump encoding genes and antimicrobial effect of Mentha longifolia and Menthol on MICs of imipenem and ciprofloxacin in clinical isolates of Acinetobacter baumannii. BMC Complement Med Ther. 2020;20.
  22. Zhang L., et al. Antibacterial activity and mode of action of mentha arvensis ethanol extract against multidrug-resistant acinetobacter Baumannii. Tropical Journal of Pharmaceutical Research. 2015;14.
  23. Coutinho H.D.M., Costa J.G.M., Lima E.O., Falcão-Silva V.S., Siqueira J.P. Enhancement of the antibiotic activity against a multiresistant Escherichia coli by Mentha arvensis L. and chlorpromazine. Chemotherapy. 2008;54.
Journal of Medicinal plants and By-Products
Volume 14, Issue 2
March and April 2025
Pages 209-214

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