Estimation of Genetic Affinity between some Tissue-planted Date Palm Taxa and Natural-planted Date Palm Taxa in Iraq using the Nucleotide Sequence of the Rbcl Gene

Document Type : Research Paper

Author

Department Biology, College of Education for Pure Sciences, University of Diyala, Iraq

Abstract

The date palm is one of the most important early trees that man took care of because of its importance in many areas, including the economy, industry, and medicine. Due to the phenotypic similarities of date palm cultivars in several traits. This study's goal is to quantify the genetic affinity of 12 taxa of tissue-planted date palm and natural-planted date palm using specific primers for the rbcl gene, The rbcl gene's nitrogenous bases were aligned, As the results of paving the nitrogenous bases of the rbcl gene showed that there are two types of mutations, translocation and transformation, and the match rate between the study samples and the samples of the gene bank were (96%, 98%, 99%). The phylogenetic tree of the current study's findings revealed that there are three primary groupings, the K1 variety serving as their common ancestor. The variations K6 and K10, K2 and K7, K3 and K4, and K8 and K11 are the most closely related. The results of the phylogenetic tree of the current study varieties and samples of the gene bank and the ancestors of 3 main groups showed that the first group included the varieties (K3, K4, K12) and that the two varieties (K3) and (K4) were more closely related, while the second main group included the variety MG946856.1, and the third main group included There were 18 cultivars, and the most closely related cultivars were (MN216504.1 with MN216503.1), (KT454753.1 with K8), (K2 with K7), and (K6 with 10).

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Journal of Medicinal plants and By-Products

Articles in Press, Accepted Manuscript
Available Online from 21 December 2024

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