Optimizing Nutrient Solution Regime to Enhance Yield, Biochemical Composition, and Antioxidant Capacity in Andrographis paniculata

Document Type : Research Paper

Authors

1 Immunoregulation Research Center, Shahed University, Tehran, Iran

2 Medicinal Plants Research Center, Shahed University, Tehran, Iran

3 Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran

4 Department of Physiology, Faculty of Medicine, Shahed University, Tehran, Iran

Abstract

The quality and quantity of the nutrient solution and its application duration are critical factors influencing essential growth processes such as photosynthesis and enzyme activity in plants. This research sought to investigate the impact of nutrient supplementation on the yield, biochemical composition, and antioxidant systems of the medicinal plant Andrographis paniculata (AP). To achieve this, an experiment was conducted using a randomized complete block design (RCBD) by four nutrient solution regimes (every day, every three days, every five days, and every week) and five repetitions. Seedlings at the 8-leaf stage were placed in pots filled with a 50:50 mixtures of sand and vermiculite, and maintained under greenhouse conditions for 30 days. The morphological analysis revealed that the highest height of the plant (55.20 cm) and leaf dry weight (6.12 g) were recorded after three days of nutrient solution application. Additionally, the comparison of root lengths indicated that the shortest and longest roots were observed after one and three days of nutrient solution application, respectively. The biochemical analysis demonstrated that the highest levels of chlorophyll, FRAP, total phenols, and flavonoids were achieved after three days of nutrient solution application. Consequently, the findings suggest that administering the nutrient solution every three days in a nutrient-deficient sandy medium is an effective strategy for enhancing the yield and physiological attributes of the A. paniculata plant.

Keywords

Main Subjects


  1. Chen X., Ren J., Yang J., Zhu Z., Chen R., Zhang L. A critical review of Andrographis paniculata. Medicinal Plant Biology. 2023;2(1):1-12.
  2. Valdiani A., Mihdzar A.K., Tan S.G., Talei D., Puad M.A., Nikzad S. Nain-e Havandi (Andrographis paniculata) present yesterday, absent today: A plenary review on underutilized herb of Iran’s pharmaceutical plants. Molecular Biology Reports. 2012;39:5409-5424.
  3. Rajani M., Shrivastava N., Ravishankara M.N. A rapid method for isolation of andrographolide from Andrographis paniculata Nees (Kalmegh). Pharmaceutical Biology. 2000;38(3):204-209.
  4. Basak A., Banik U.K., Basak S., Seiah N.G., Li S. Evaluation of anti-proprotein convertase activity of diterpene andrographolid derived products. Regulation of carcinogenesis, angiogenesis and metastasis by the proprotein convertases (PCs): a new potential strategy in cancer therapy, Springer. 2006:137-154.
  5. Ko H.C., Wei B.L, Chiou W.F. The effect of medicinal plants used in Chinese folk medicine on RANTES secretion by virus-infected human epithelial cells. Journal of Ethnopharmacology. 2006;107(2):205-210.
  6. Chun J.Y., Tummala R., Nadiminty N., Lou W., Liu C. Andrographolide, an herbal medicine, inhibits interleukin-6 expression and suppresses prostate cancer cell growth Genes & Cancer. 2010;1(8):868-876.
  7. Dumrongsak P., Nadege B., Catherine A., Georges M., Alail B., Helene M. Effect of Andrographis paninculata extract and andrographolide on hepatic cyt P450 mRNA expression and monoxygenase activity after in vivo administration to rats and in vitro in rat and human hepatic cultures. Chemico Biological Interactions. 2009;179(2-3):247-255.
  8. Chao W.W., Kuo Y.H., Lin B.F. Anti-inflammatory activity of new compounds from Andrographis paniculata by NF-KB transactivation inhibition. J Agric Food Chem 2010;58(4):2505-12.
  9. Kumar B., Verma S.K., Singh H. Effect of temperature on seed germination parameters in Kalmegh (Andrographis paniculata Wall. ex Nees.) Ind Crops Prod. 2011;34:1241-1244.
  10. Bayer A., Whitaker K., Chappell M., Ruter J., van Iersel M., editors. Effect of irrigation duration and fertilizer rate on plant growth, substrate solution EC and leaching volume. International Symposium on Growing Media and Soilless Cultivation 1034; 2013.
  11. Schjoerring J.K., Cakmak I., White P.J. Plant nutrition and soil fertility: synergies for acquiring global green growth and sustainable development. Springer; 2019; 1-6.
  12. Petropoulos S.A., El-Nakhel C., Graziani G., Kyriacou M.C., Rouphael Y. The effects of nutrient solution feeding regime on yield, mineral profile, and phytochemical composition of spinach microgreens. Horticulturae. 2021;7(7):162.
  13. Tang L. Soil Fertility, Plant Nutrition and Nutrient Management. MDPI; 2024;1- 34.
  14. Withers P.J., Doody D.G., Sylvester-Bradley R. Achieving sustainable phosphorus use in food systems through circularisation. Sustainability. 2018;10(6):1804.
  15. Rychter A.M., Rao I. Role of phosphorus in photosynthetic carbon metabolism. Handbook of photosynthesis. 2005;2:123-48.
  16. Rao I.M., Miles J.W., Beebe SE, Horst WJ. Root adaptations to soils with low fertility and aluminium toxicity. Annals of Botany. 2016;118(4):593-605.
  17. Smith M.R., Reis Hodecker B.E., Fuentes D., Merchant A. Investigating nutrient supply effects on plant growth and seed nutrient content in common bean. Plants. 2022;11(6):737.
  18. Tozlu I., Moore G.A., Guy C.L. Effects of increasing NaCl concentration on stem elongation, dry mass production, and macro-and micro-nutrient accumulation in Poncirus trifoliata. Functional plant biology. 2000;27(1):35-42.
  19. Garcia C.B., Grusak M.A. Mineral accumulation in vegetative and reproductive tissues during seed development in Medicago truncatula. Frontiers in Plant Science. 2015;6:155788.
  20. Talei D., Mihdzar A.K., Khanif M.Y., Saad M.S., Valdiani A.R. Effects of different surface sterilizers on seed germination and contamination of king of bitters (Andrographis paniculata Nees.). American-Eurasian journal of agricultural and environmental sciences. 2011;10:639-643.
  21. Talei D., Valdiani A., Maziah M., Sagineedu S.R., Abiri R. Salt stress-induced protein pattern associated with photosynthetic parameters and andrographolide content in Andrographis paniculata Nees. Bioscience, biotechnology, and biochemistry. 2014;(ahead-of-print):1-8.
  22. Bates L.S., Waldren R.P., Teare I.D. Rapid determination of free proline for water-stress studies. Plant and Soil. 1973;39(1):205-207.
  23. Wong S.P., Leong L.P., Koh J.H.W. Antioxidant activities of aqueous extracts of selected plants. Food chemistry. 2006;99(4):775-783.
  24. Marinova D., Ribarova F., Atanassova M. Total phenolics and total flavonoids in Bulgarian fruits and vegetables. Journal of the University of Chemical Technology and Metallurgy. 2005;40(3):255-260.
  25. Martin C.C.S. Enhancing soil suppressiveness using compost and compost tea. Organic amendments and soil suppressiveness in plant disease management: Springer; 2015;25-49.
  26. Ma Q., Bell R., Biddulph B. Potassium application alleviates grain sterility and increases yield of wheat (Triticum aestivum) in frost-prone Mediterranean-type climate. Plant and Soil. 2019;434:203-16.
  27. Tränkner M., Tavakol E., Jákli B. Functioning of potassium and magnesium in photosynthesis, photosynthate translocation and photoprotection. Physiologia plantarum. 2018;163(3):414-431.
  28. El‐Nakhel C., Pannico A., Kyriacou M.C., Giordano M., De Pascale S., Rouphael Y. Macronutrient deprivation eustress elicits differential secondary metabolites in red and green‐pigmented butterhead lettuce grown in a closed soilless system. Journal of the Science of Food and Agriculture. 2019;99(15):6962-6972.
  29. Murphy C., Pill W. Cultural practices to speed the growth of microgreen arugula (roquette; Eruca vesicaria subsp. sativa). The Journal of Horticultural Science and Biotechnology. 2010;85(3):171-176.
  30. Wieth A.R., Pinheiro W.D., Duarte TdS. Purple cabbage microgreens grown in different substrates and nutritive solution concentrations. Revista Caatinga. 2020;32:976-85.
  31. Pannico A., Graziani G., El-Nakhel C., Giordano M., Ritieni A., Kyriacou M.C., et al. Nutritional stress suppresses nitrate content and positively impacts ascorbic acid concentration and phenolic acids profile of lettuce microgreens. Italus Hortus. 2020;27:41-52.
  32. Becker C., Urlić B., Jukić Špika M., Kläring H.P., Krumbein A., Baldermann S., et al. Nitrogen limited red and green leaf lettuce accumulate flavonoid glycosides, caffeic acid derivatives, and sucrose while losing chlorophylls, β-carotene and xanthophylls. PloS one. 2015;10(11):e0142867.
  33. Pereira C., Dias M.I., Petropoulos S.A., Plexida S., Chrysargyris A., Tzortzakis N., et al. The effects of biostimulants, biofertilizers and water-stress on nutritional value and chemical composition of two spinach genotypes (Spinacia oleracea L.). Molecules. 2019;24(24):4494.
  34. El Boukhari M.E.M., Barakate M., Drissi B., Bouhia Y., Lyamlouli K. Seaweed extract biostimulants differentially act in mitigating drought stress on faba bean (Vicia faba L.). Journal of Plant Growth Regulation. 2023;42(9):5642-5652.
  35. Karray-Bouraoui N., Harbaoui F., Rabhi M., Jallali I., Ksouri R., Attia H., et al. Different antioxidant responses to salt stress in two different provenances of Carthamus tinctorius L. Acta Physiologiae Plantarum. 2011;33:1435-1444.
  36. Arif Y., Singh P., Siddiqui H., Bajguz A., Hayat S. Salinity induced physiological and biochemical changes in plants: An omic approach towards salt stress tolerance. Plant Physiology and Biochemistry. 2020;156:64-77.
  37. Zhang Q., Dai W. Plant response to salinity stress. Stress physiology of woody plants: CRC Press; 2019;155-173.