Phytochemical Profiling of Methanolic Leaf extract of Vietnamese Murraya koenigii, a Highly Valued Traditional Medicine, using UHPLC‐Q‐TOF‐MS/MS

Document Type : Research Paper

Authors

1 Tra Vinh University, Tra Vinh City 700000, Vietnam

2 Faculty of Pharmacy, Nguyen Tat Thanh University, Ho Chi Minh City 700000, Vietnam

3 Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh City 700000, Vietnam

4 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia

Abstract

Murraya koenigii (L.) Spreng (curry tree) is a tropical to subtropical tree originally from South Asia. The leaves of curry trees are an essential ingredient of Indian cuisine and are used as a flavoring in many dishes. The leaves are also widely used in Indian traditional medicine and possess the therapeutic potential due to the high content of biologically active compounds, including but not limited to flavonoids, steroids, alkaloids, phenolic compounds, etc. These compounds exhibit antioxidant, hypoglycemic, anticancer, antibacterial, and some other curative properties. It is well known that phytochemical composition is dependent on plant habitat. M. koenigii grows in Vietnam, where it is called “сà ri,” and is used in its cuisine. However, there is no data about the phytochemical profile of this plant from Vietnam. In this study, the extract of curry tree leaves was studied. For long-term methanol extraction, a maceration technique for 7 days was used. The extract was analyzed by UV-spectroscopy and ultra‐high‐performance liquid chromatography‐quadrupole time‐of‐fight tandem mass spectrometry (UHPLC‐Q‐TOF‐MS/MS). In the end, about two hundred matches with known substances including flavonoids, alkaloids, steroids, terpenoids, phenolic, and other compounds were identified in M. koenigii extract. In general, the phytochemical composition of Vietnamese M. koenigii resembles those of plants from other countries. However, several new compounds of unknown structure were found. Therefore, a further more detailed study of M. koenigii and other Murraya species native to Vietnam is needed.

Keywords

Main Subjects