Burn Wound Healing Effect of Traditionally Used Persian Medicine Ointment Containing Herbal Extracts and Yogurt Butter

Document Type : Research Paper

Authors

1 Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

2 Research Center for Evidence-Based Medicine, Health Management and Safety Promotion Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran

3 Iranian EBM Centre: A Joanna Briggs Institute Affiliated Group, Tabriz, Iran

4 Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran

5 Department of Food Hygiene, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies (AUSMT), Amol, Iran

6 St George and Sutherland Clinical School, UNSW, Sydney, Australia

7 School of Nursing and Midwifery, Alborz University of Medical Sciences, Karaj, Iran

8 Evidence-based Phytotherapy and Complementary Medicine Research Center, Alborz University of Medical Sciences, Karaj, Iran

9 Qom University of Medical Sciences and Health Services, Qom, Iran

10 Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran

Abstract

This study aimed to assess the burn healing efficacy of this traditionally used formulation (herbal ointment) in deep second-degree burns in a Florida white rabbit model. On the dorsal surface of the two rabbits, six burn wounds of the same size were inflicted. Herbal ointment was applied to the wounds on the right side, and silver sulfadiazine was applied to the wounds on the left side. On the 5th and 18th day of the treatment, the burn wound site was excised in a square shape with a narrow surrounding healthy skin for histopathological examination. At the end of day 18 of the treatment period, all the ulcers in the traditional herbal ointment group were covered entirely with epithelial tissue, while in the silver sulfadiazine group, the mean and SD of the ulcer diameter were (1.4 ± 0.1). The duration of pain, blister, and open wound in the silver sulfadiazine group was 6, 8, and 18 days; however, in the traditional herbal ointment group, on the second day, none of these complications were observed. Regarding the pathological findings, epithelial tissue renewal on day 18th was 6.63 ± 0.74 in the traditional herbal ointment group compared to 4.93 ± 0.48 in the silver sulfadiazine (P< 0.05). The findings indicate that the traditional herbal ointment can reduce the duration of the wound healing process as well as the complications, including pain and blister formation. The pathological findings also suggest better epithelial tissue renewal in the traditional herbal ointment group.

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Main Subjects

References

  1. Jeschke M.G., van Baar M.E., Choudhry M.A., Chung K.K., Gibran N.S., Logsetty S. Burn injury. Nature Reviews Disease Primers. 2020;6(11):1-25.
  2. Burgess M., Valdera F., Varon D., Kankuri E., Nuutila K. The immune and regenerative response to burn injury. Cells. 2022;11(19):3073.
  3. Li H.-L., Deng Y.-T., Zhang Z.-R., Fu Q.-R., Zheng Y.-H., Cao X.-M., Nie J., Fu L.-W., Chen L.-P., Xiong Y.-X. Evaluation of effectiveness in a novel wound healing ointment-crocodile oil burn ointment. African Journal of Traditional. Complementary and Alternative Medicines. 2017;14(1):62-72.
  4. Manginstar C.O., Tallei T.E., Niode N.J., Salaki C.L., Hessel S.S. Therapeutic potential of propolis in alleviating inflammatory response and promoting wound healing in skin burn. Phytotherapy Research. 2024;38(2):856-79.
  5. Cancio L.C. Topical antimicrobial agents for burn wound care: history and current status. Surgical Infections. 2021;22(1):3-11.
  6. Fuller F.W. The side effects of silver sulfadiazine. Journal of Burn Care & Research. 2009;30(3):464-70.
  7. Xie S., Han J., Xie W., Luo G., Shi X., Wang H., Hou H., He X., Hu X., Ji P. Comparing collagenase and silver sulfadiazine in deep second-degree burn treatment. Archives of Dermatological Research. 2024;316(7):417.
  8. Nojani M.R., Tabarraei H., Tabari P.Z., Saberfard D., Dineli A., Mosavi S.S., Mojtahedzadeh S.M., Sabbaghi S.S., Jahandideh A., Hassanzadeh S. Evaluation of the Effects of a Traditional Combined Ointment on Healing Process of Burn Wounds in Comparison to Silver Sulfadiazine. 2024.
  9. Gümüş K., Özlü Z.K. The effect of a beeswax, olive oil and Alkanna tinctoria (L.) Tausch mixture on burn injuries: An experimental study with a control group. Complementary Therapies in Medicine. 2017;34:66-73.
  10. Bayir Y., Un H., Ugan R.A., Akpinar E., Cadirci E., Calik I., Halici Z. The effects of Beeswax, Olive oil and Butter impregnated bandage on burn wound healing. Burns. 2019;45(6):1410-17.
  11. Shahzad M.N., Ahmed N. Effectiveness of Aloe Vera gel compared with 1% silver sulphadiazine cream as burn wound dressing in second degree burns. Journal of the Pakistan Medical Association. 2013;63(2):225-30.
  12. Xu F.-W., Lv Y.-L., Zhong Y.-F., Xue Y.-N., Wang Y., Zhang L.-Y., Hu X., Tan W.-Q. Beneficial effects of green tea EGCG on skin wound healing: A comprehensive review. Molecules. 2021;26(20):6123.
  13. Fallah Huseini H., Abdolghaffari A.H., Ahwazi M., Jasemi E., Yaghoobi M., Ziaee M. Topical application of Teucrium polium can improve wound healing in diabetic rats. The International Journal of Lower Extremity Wounds. 2020;19(2):132-38.
  14. Fallah Huseini H., Yaghoobi M., Fallahi F., Boroumand F., Ezzati M.H., Tabatabaei S.M., Sotvan H., Ahvazi M., Badiee Aval S., Ziaee M. Topical Administration of Teucrium polium on Diabetic Foot Ulcers Accelerates Healing: A Placebo-Controlled Randomized Clinical Study. The International Journal of Lower Extremity Wounds. 2024;23(2):238-46.
  15. Cheppudira B., Fowler M., McGhee L., Greer A., Mares A., Petz L., Devore D., Loyd D.R., Clifford J.L. Curcumin: a novel therapeutic for burn pain and wound healing. Expert Opin Investig Drugs. 2013;22(10):1295-303.
  16. Avsar U., Halici Z., Akpinar E., Yayla M., Avsar U., Harun U., Harun U., Hasan Tarik A., Bayraktutan Z. The effects of argan oil in second-degree burn wound healing in rats. Ostomy Wound Manage. 2016;62(3):26-34.
  17. Edraki M., Akbarzadeh A., Hosseinzadeh M., Tanideh N., Salehi A., Koohi-Hosseinabadi O. Healing Effect of Sea Buckthorn, Olive Oil, and Their Mixture on Full-Thickness Burn Wounds. Advances in Skin & Wound Care. 2014;27(7):317-23.
  18. Armand N., Amiri H., Ismaili A. The effects of spraying methanol solution and drought stress on level of antioxidant enzyme activity, growth, biomass and yield of beans (Phaseolus vulgaris L. cv. COS16). Legume Research-An International Journal. 2016;39(3):385-95.
  19. Rad A., Abbasi A., Javadi A., Pourjafar H., Javadi M., Khaleghi M. Comparing the microbial quality of traditional and industrial yoghurts. Biointerface Research in Applied Chemistry. 2020;10(4):6020-25.
  20. Moustafa A., Atiba A. The effectiveness of a mixture of honey, beeswax and olive oil in treatment of canine deep second-degree burn. Global Veterinaria. 2015;14(2):244-50.
  21. Cicerale S., Lucas L., Keast R. Antimicrobial, antioxidant and anti-inflammatory phenolic activities in extra virgin olive oil. Current Opinion in Biotechnology. 2012;23(2):129-35.
  22. Farooq M.A., Ali S. Therapeutic applications of garlic and turmeric for the diabetic wound healing in mice. 2022.
  23. Zhang P., Zou B., Liou Y.-C., Huang C. The pathogenesis and diagnosis of sepsis post burn injury. Burns & Trauma. 2021;9:tkaa047.
  24. Jordan K.C., Di Gennaro J.L., von Saint André-von Arnim A., Stewart B.T. Global trends in pediatric burn injuries and care capacity from the World Health Organization Global Burn Registry. Frontiers in Pediatrics. 2022;10:954995.
  25. Kaufman T., Lusthaus S.N., Sagher U., Wexler M.R. Deep partial skin thickness burns: A reproducible animal model to study burn wound healing. Burns. 1990;16(1):13-16.
  26. Johnson C. Research Tools for the Measurement of Pain and Nociception. Animals (Basel). 2016;6(11).
  27. Kilkenny C., Browne W.J., Cuthill I.C., Emerson M., Altman D.G. The ARRIVE guidelines animal research: reporting in vivo experiments. PLoS Biology. 2010;8(6):e1000412.
  28. Wang P.H., Huang B.S., Horng H.C., Yeh C.C., Chen Y.J. Wound healing. Journal of the Chinese Medical Association. 2018;81(2):94-101.
  29. Sorg H., Tilkorn D.J., Hager S., Hauser J., Mirastschijski U. Skin Wound Healing: An Update on the Current Knowledge and Concepts. European Surgical Research. 2017;58(1-2):81-94.
  30. Harding C.R., Rawlings A.V. 18 Effects of Natural Moisturizing Factor and Lactic Acid Isomers on Skin Function. Dry Skin and Moisturizers. 2000:187.
  31. Nguyen T.T.T., Ghosh C., Hwang S.-G., Tran L.D., Park J.S. Characteristics of curcumin-loaded poly (lactic acid) nanofibers for wound healing. Journal of Materials Science. 2013;48:7125-33.
  32. Saravanan P., Balachander N., K K.R.S., S S., S R. Anti-inflammatory and wound healing properties of lactic acid bacteria and its peptides. Folia Microbiologica. 2023;68(3):337-53.
  33. Porporato P.E., Payen V.L., De Saedeleer C.J., Préat V., Thissen J.-P., Feron O., Sonveaux P. Lactate stimulates angiogenesis and accelerates the healing of superficial and ischemic wounds in mice. Angiogenesis. 2012;15:581-92.
  34. Wang Z., Zhao F., Xu C., Zhang Q., Ren H., Huang X., He C., Ma J., Wang Z. Metabolic reprogramming in skin wound healing. Burns & Trauma. 2024;12:tkad047.
  35. Abdolhosseinzadeh E., Dehnad A.R., Pourjafar H., Homayouni A., Ansari F. The Production of Probiotic Scallion Yogurt: Viability of Lactobacillus Acidophilus Freely and Microencapsulated in the Product. Carpathian Journal of Food Science & Technology. 2018;10(3).
  36. Rad A.H., Hosseini S., Pourjafar H. Postbiotics as dynamic biological molecules for antimicrobial activity: A mini-review. Biointerface Research in Applied Chemistry. 2022;12(5):6543-56.
  37. Homayouni-Rad A., Azizi A., Oroojzadeh P., Pourjafar H. Kluyveromyces marxianus as a probiotic yeast: A mini-review. Current Nutrition & Food Science. 2020;16(8):1163-69.
  38. Melguizo-Rodríguez L., de Luna-Bertos E., Ramos-Torrecillas J., Illescas-Montesa R., Costela-Ruiz V.J., García-Martínez O. Potential effects of phenolic compounds that can be found in olive oil on wound healing. Foods. 2021;10(7):1642.
  39. Akbik D., Ghadiri M., Chrzanowski W., Rohanizadeh R. Curcumin as a wound healing agent. Life Sciences. 2014;116(1):1-7.
  40. Mahmudi G., Nikpour M., Azadbackt M., Zanjani R., Jahani M., Aghamohammadi A., Jannati Y. The impact of turmeric cream on healing of caesarean scar. The West Indian Medical Journal. 2016;64(4):400.
  41. Nong Y., Maloh J., Natarelli N., Gunt H.B., Tristani E., Sivamani R.K. A review of the use of beeswax in skincare. Journal of Cosmetic Dermatology. 2023;22(8):2166-73.
  42. Shuban F.S., Idris M.I. Fabrication of natural and bio-based wound healing material from alginate and beeswax. Paper Presented at the AIP Conference Proceedings. 2024.
  43. Farzadinia P., Jofreh N., Khatamsaz S., Movahed A., Akbarzadeh S., Mohammadi M., Bargahi A. Anti-inflammatory and wound healing activities of Aloe vera, honey and milk ointment on second-degree burns in rats. The International Journal of Lower Extremity Wounds. 2016;15(3):241-47.
Journal of Medicinal plants and By-products
Volume 14, Issue 6
November and December 2025
Pages 632-638

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