Upregulation of Myrosinase and TGG Genes in Capparis ovata Under Environmental Radon Exposure

Tadili, Sahar; Asghari, Ali; Hosseinpour Azad, Noraddin; Hashemipetroudi, Seyed Hamidreza

doi:10.22034/jmpb.2025.369852.2000

Upregulation of Myrosinase and TGG Genes in Capparis ovata Under Environmental Radon Exposure

Articles in Press

Document Type : Research Paper

Authors

¹ Faculty of Agricultural Sciences and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran

² Department of Plant Production and Genetics, Faculty of Agricultural Sciences and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran

³ Department of Plant Sciences and Medicinal Plants, Meshginshahr Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran

⁴ Department of Genetic Engineering and Biology, Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran

10.22034/jmpb.2025.369852.2000

Abstract

Radon is a naturally occurring radioactive gas and a significant contributor to environmental ionizing radiation. While its health impacts in humans are well documented, its effects on plant molecular biology remain largely unexplored. Myrosinase, a key enzyme in Capparis ovata and other Brassicas, is involved in plant defense against herbivores by hydrolyzing glucosinolate. This study investigates the impact of environmental radon exposure on the expression of the Myrosinase and TGG (Thioglucoside glucohydrolase) genes in Capparis ovata.
Capparis ovata samples were collected from sites with different elevations and background radon levels in selected regions. Environmental radon concentrations were mapped using an alphabet PQ2000 Pro detector. The total RNA was extracted from leaf tissue, and cDNA of Myrosinase and TGG genes was synthesized for the real-time PCR (qRT-PCR) analysis. The level of genes expression was normalized for actin genes as a housekeeping gene and the relative change was analyzed using 2^−ΔCT method. Statistical differences was assessed by independent T-test. Radon-exposure against on Capparis ovata display an important upregulation of both Myrosinase and TGG genes compared to control. Myrosinase-related genes showed a notable increase in fold change, while TGG gene expression was highly variable, but was largely high. These findings suggest that the environmental radon acts as a potent abiotic stressor, which triggers molecular defense reactions in the Capparis ovata. This study provides the first evidence that environmental radon exposure contributes to the expression of the significant defense-related genes in the Capparis ovata, which contains implications for plant adaptation, ecological interaction, and quality of plant-related products in radon-contaminate areas. As a result, radioactive environmental contaminants highlight the need for further research on the underlying molecular mechanisms of plant reactions.

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