The Effect of Bio Fertilizer and Thermal Accumulation on the Flavonoid Content of Oat (Avena sativa L.) Grown in a Sandy Soil Texture

Document Type : Research Paper

Authors

1 College of Pharmacy, University of Alkafeel, Najaf, Iraq

2 Department of Biology, College of Science, University of Kerbala, Karbala, Iraq

3 Department of Pharmacognosy College of Pharmacy University of Alkafeel, Iraq

4 Al-Furat Al-Awsat Tech. University, Almusaib Technical College, Iraq

Abstract

This study investigates the effects of biofertilizers and thermal accumulation on the flavonoid content of oat (Avena sativa L.) cultivars grown in sandy soil. A factorial field experiment was conducted in Al-Musaib city (Babil province, Iraq) during the 2023–2024 growing season to evaluate the influence of three factors: oat cultivars (Shafa, Hamel, and Gazania); biofertilizer treatments including Trichoderma harzianum and Azospirillum spp.; and sowing dates (November 1, 15, and 30, 2023) to study thermal accumulation effects. The experiment followed a randomized complete block design with three replicates. Results showed significant effects of cultivar and biofertilizer treatments on most flavonoid compounds, except catechin which was not significantly affected. Notably, the Gazania cultivar exhibited the highest kaempferol, myricetin, and rutin contents, especially when treated with both fungal and bacterial biofertilizers. Hamel showed higher apigenin levels, particularly under bacterial stimulation and late sowing. The optimum sowing date for maximizing flavonoid content was November 15. The study highlights the potential of using specific biofertilizer treatments and sowing dates to enhance the nutritional and medicinal qualities of oats through flavonoid enrichment.

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  1. Lee W.S., Lee H.-J., Yang J.Y., Shin H.-L., Choi S.-W., Kim J.-K., Seo W.D., Kim E.H. The potential neuroprotective effects of extracts from oat seedlings against Alzheimer’s disease. Nutrients. 2022; 14(19): 4103.
  2. Verardo V., Serea C., Segal R., Caboni M.F. Free and bound minor polar compounds in oats: Different extraction methods and analytical determinations. Journal of Cereal Science. 2011; 54(2): 211-217.
  3. Shvachko N.A., Loskutov I.G., Semilet T.V., Popov V.S., Kovaleva O.N., Konarev A.V. Bioactive Components in Oat and Barley Grain as a Promising Breeding Trend for Functional Food Production. Molecules. 2021; 26(8).
  4. Tanu A., Rattey A., Fletcher A., Rich S., Taylor A., Veneklaas E. Enhancing deep sowing success: genetic diversity in mesocotyl and coleoptile length, and field establishment of oats (Avena sativa). Functional Plant Biology. 2025; 52: 24321.
  5. https://www.fao.org/faostat/en/#home, 2023;
  6. Lobell D.B., Hammer G.L., Chenu K., Zheng B., McLean G., Chapman S.C. The shifting influence of drought and heat stress for crops in northeast Australia. Global Change Biology. 2015; 21(11): 4115-4127.
  7. Ndlovu E., van Staden J., Maphosa M. Morpho-physiological effects of moisture, heat and combined stresses on Sorghum bicolor [Moench (L.)] and its acclimation mechanisms. Plant Stress. 2021; 2: 100018.
  8. Mirzaei N., Fahmide L., Fazeli-Nasab B. Evaluation of morphological traits of leaf and fruit in Ziziphus mauritiana L. genotypes in southern Iran. Crop Science Research in Arid Regions. 2024; 6(1): 187-202.
  9. Pahlavan A., Solouki M., Fakheri B., Fazeli-Nasab B. The use of morphological and physiological characteristics and regression step by step to the evaluation of the different ecotypes of Iranian Malva (Malva sylvestris L.). Crop Science Research in Arid Regions. 2024; 6(3): 105-120.
  10. Dawar S., Wahab S., Tariq M., Zaki M.J. Application of Bacillus species in the control of root rot diseases of crop plants. Archives of Phytopathology and Plant Protection. 2010; 43(4): 412-418.
  11. Al-Samarrai I.K., Rahi H.S. Effect of inoculation with Azotobacter and Azosperillium bacteria on the absorption of some nutrients, the concentration of plant hormones, and the growth of tomato transplantss. Iraqi Journal of Agricultural Sciences. 2006; 37(3): 27-32.
  12. Kumar R., Kumawat N., Sahu Y.K. Role of biofertilizers in agriculture. Popular kheti. 2017; 5(4): 63-66.
  13. Vessal S., Salehi-Sardoei A., Fazeli-Nasab B., Shafi N., Shameem N., Parray J.A. Azospirillum, a Free-Living Nitrogen-Fixing Bacterium: Smart Agriculture and Sustainable Exploitation. In Parray J A (Ed.), Progress in Soil Microbiome Research (pp. 365-399). Cham: Springer Nature Switzerland. 2024
  14. Li K.Y., Liang J.J., Peng Y.F., Ling X.F., Cai Y.T., Yi R.H. Leaf Spots on bodhi tree (Ficus religiosa) caused by Diaporthe tulliensis. Plant Disease. 2022.
  15. Yang M., Zeng W., Zhang Y., Hu L., Xi J., Guo Y., Li B., Dong X., Wang J., Gao Q., Liang T. Leaf grey spot caused by Arcopilus aureus on tobacco in China. Plant Dis. 2024.
  16. Prasad A.A., Babu S. Compatibility of Azospirillum brasilense and Pseudomonas fluorescens in growth promotion of groundnut ( Arachis hypogea L.). An Acad Bras Cienc. 2017; 89(2): 1027-1040.
  17. Hu Q.-L., Zhang L.-J., Li Y.-N., Ding Y.-J., Li F.-L. Purification and anti-fatigue activity of flavonoids from corn silk. International Journal of Physical Sciences. 2010; 5(4): 321-326.
  18. Ngamsuk S., Huang T.-C., Hsu J.-L. Determination of phenolic compounds, procyanidins, and antioxidant activity in processed Coffea arabica L. leaves. Foods. 2019; 8(9): 389.
  19. Al-Rawi K.M., Khalaf Allah A.M. Design and analysis of agricultural experiments. El Mousel University, Iraq. 1980; 19: 487.
  20. Sarker U., Hossain M.N., Iqbal M.A., Oba S. Bioactive Components and Radical Scavenging Activity in Selected Advance Lines of Salt-Tolerant Vegetable Amaranth. Frontiers in Nutrition. 2020; 7: 587257.
  21. Liaudanskas M., Noreikiene I., Zymone K., Juodyte R., Zvikas V., Janulis V. Composition and Antioxidant Activity of Phenolic Compounds in Fruit of the Genus Rosa L. Antioxidants (Basel). 2021; 10(4).
  22. Hosseinpour T., Ahmadi A., Mohammadi F., Drikvand R. The effect of seed rate on grain yield and its components of wheat cultivars in rain fed Conditions. Applied Field Crops Research. 2014; 27(105): 101-110.
  23. Altomare C., Norvell W., Björkman T., Harman G. Solubilization of phosphates and micronutrients by the plant-growth-promoting and biocontrol fungus Trichoderma harzianum Rifai 1295-22. Applied and Environmental Microbiology. 1999; 65(7): 2926-2933.
  24. Harman G.E. Myths and dogmas of biocontrol changes in perceptions derived from research on Trichoderma harzinum T-22. Plant Disease. 2000; 84(4): 377-393.
  25. Chen J.-H. The combined use of chemical and organic fertilizers and/or biofertilizer for crop growth and soil fertility. Paper presented at the International workshop on sustained management of the soil-rhizosphere system for efficient crop production and fertilizer use. 2006
  26. Richardson A.E., Barea J.-M., McNeill A.M., Prigent-Combaret C. Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant and Soil. 2009; 321(1): 305-339.
  27. Zeng Q., Ding X., Wang J., Han X., Iqbal H.M.N., Bilal M. Insight into soil nitrogen and phosphorus availability and agricultural sustainability by plant growth-promoting rhizobacteria. Environmental Science and Pollution Research International. 2022; 29(30): 45089-45106.
  28. Jacobs D.I., Snoeijer W., Hallard D., Verpoorte R. The Catharanthus alkaloids: pharmacognosy and biotechnology. Current Medicinal Chemistry. 2004; 11(5): 607-628.
  29. Nazer Kakhki S.H., Taghaddosi M.V., Moini M.R., Naseri B. Predict bean production according to bean growth, root rots, fly and weed development under different planting dates and weed control treatments. Heliyon. 2022; 8(11): e11322.
  30. Islam S.S., Billah A., Hasan A.K., Karim R., Khomphet T. Evaluating the impact of Trichoderma biofertilizer and planting dates on mustard yield performance using the InfoCrop growth model. PLoS One. 2023; 18(5): e0285482.
  31. Ei E., Park H.H., Kuk Y.I. Effects of Plant Extracts on Growth Promotion, Antioxidant Enzymes, and Secondary Metabolites in Rice (Oryza sativa) Plants. Plants (Basel). 2024; 13(19).
  32. Shim D., Lee K.-J., Lee B.-W. Response of phenology- and yield-related traits of maize to elevated temperature in a temperate region. The Crop Journal. 2017; 5(4): 305-316.
  33. Sohag A.A.M., Tahjib-Ul-Arif M., Brestic M., Afrin S., Sakil M.A., Hossain M.T., Hossain M.A., Hossain M.A. Exogenous salicylic acid and hydrogen peroxide attenuate drought stress in rice. Journal of Soil, Plant and Environment. 2020; 66(1): 7-13.