Medicinal herbs have attracted considerable attention as natural edibles to enhance growth performance and meat quality in ruminants. This study was conducted to evaluate the impact of dietary supplementation with medicinal herbs, peppermint (Mentha piperita) or thyme (Thymus vulgaris), on production performance and meat quality in Sanjabi fattening lambs. The experimental diets were control diet without medicinal herbs, basal diet contained 3% peppermint powder, and basal diet contained 3% thyme powder.Results showed that peppermint supplementation in lambs improved average daily gain (298.15 g/day), while the control group showed the lowest daily gain (228.70 g/day, P < 0.05). Dietary supplementation with peppermint and thyme powders lowered rumen pH and reduced ammonia-nitrogen concentrations compared to the control group (P < 0.05). The peppermint-supplemented group exhibited significantly higher final live weight, rumen capacity, and both hot and cold carcass weights compared to the thyme and control groups (P < 0.05). Overall, incorporating peppermint and thyme powders into the diet enhanced growth performance, decreased ruminal ammonia-nitrogen levels, and improved carcass traits and meat quality in Sanjabi fattening lambs. These findings highlight the potential of medicinal herbs as effective natural feed additives to boost small ruminant production efficiency.
Matheou A., Abousetta A., Pascoe A.P., Papakostopoulos D., Charalambous L., Panagi S., Johnson E.O. Antibiotic Use in Livestock Farming: A Driver of Multidrug Resistance?. Microorganisms. 2025; 13(4): 779.
Antonius A., Pazla R., Putri E.M., Negara W., Laia N., Ridla M., Marta Y. Effectiveness of herbal plants on rumen fermentation, methane gas emissions, in vitro nutrient digestibility, and population of protozoa. Veterinary World. 2023; 16(7): 1477.
Rahman M.A., Redoy M.R.A., Shuvo A.A. S., Chowdhury R., Hossain E., Sayem S. M., Al-Mamun M. Influence of herbal supplementation on nutrient digestibility, blood biomarkers, milk yield, and quality in tropical crossbred cows. PLoS One. 2024; 19(11): e0313419.
Alem W.T. Effect of herbal extracts in animal nutrition as feed additives. Heliyon. 2024; 10(3).
Wang X., Wang Y., Feng M., Li J., Liu Z., Fu L., Qin J. Herbal formula alleviates heat stress by improving physiological and biochemical attributes and modulating the rumen microbiome in dairy cows. Front. Vet. Sci. 2025; 12: 1558856.
Bąkowski M., Kiczorowska B. Probiotic microorganisms and herbs in ruminant nutrition as natural modulators of health and production efficiency–a review. Ann. Anim. Sci. 2021; 21(1): 3-28.
Elbaz A.M., El-Hawy A.S., Salem F.M., Lotfy M.F., Ateya A., Alshehry G., Abdelhady A. Y. Dietary incorporation of melittin and clove essential oil enhances performance, egg quality, antioxidant status, gut microbiota, and MUC-2 gene expression in laying hens under heat stress conditions. Ital. J. Anim. Sci. 2025; 24(1): 1762-1773.
Ahmed M.G., Elwakeel E.A., El-Zarkouny S.Z., Al-Sagheer A.A. Environmental impact of phytobiotic additives on greenhouse gas emission reduction, rumen fermentation manipulation, and performance in ruminants: an updated review. Environ. Sci. Pollut. Res. 2024; 31(26): 37943-37962.
Feknous I., Ait Saada D., Boulahlib C.Y., Alessandroni L., Souidi S. W., Ait Chabane O., Gagaoua M. Poultry meat quality preservation by plant extracts: an overview. Sci. J. Meat Technol. 2023; 64(3): 80-101.
Piao M., Tu Y., Zhang N., Diao Q., Bi Y. Advances in the application of phytogenic extracts as antioxidants and their potential mechanisms in ruminants. Antioxidants. 2023; 12(4): 879.
Carvalho F.A.L.D. Natural antioxidants and lipid profile improvement in lamb meat products (Doctoral dissertation). Universidade de São Paulo, 2020.
Ponnampalam E. N., Kiani A., Santhiravel S., Holman B. W., Lauridsen C., Dunshea F. R. The importance of dietary antioxidants on oxidative stress, meat and milk production, and their preservative aspects in farm animals: Antioxidant action, animal health, and product quality—Invited review. Animals.2022; 12(23): 3279.
Toral P. G., Monahan F. J., Hervás G., Frutos P., Moloney A. P. Modulating ruminal lipid metabolism to improve the fatty acid composition of meat and milk. Challenges and opportunities. Anim. 2018; 12(s2): s272-s281.
Nutrient Requirements of Small Ruminants: Sheep, Goats, Cervids, and New World Camelids. National Academy of Science, Washington, DC; 2007.
Palhares Campolina J., Gesteira Coelho S., Belli A. L., Samarini Machado F., Pereira R., Tomich T., Magalhães Campos M. Effects of a blend of essential oils in milk replacer on performance, rumen fermentation, blood parameters, and health scores of dairy heifers. PLoS One. 2021; 16(3): e0231068.
Stefańska B., Gąsiorek M., Kański J., Komisarek J., Nowak W. Comparison of pH, volatile fatty acids, and ammonia in preweaning and postweaning ruminal fluid samples obtained via rumenocentesis and stomach tube from dairy calves. Livest. Science. 2019; 230: 103822.
Ellison M. J., Cockrum R. R., Means W. J., Meyer A. M., Ritten J., Austin K.J., Cammack K.M. Effects of feed efficiency and diet on performance and carcass characteristics in growing wether lambs. Small Rumin. Res. 2022; 207: 106611.
AOAC International. Official Methods of Analysis of AOAC International (17th ed.). AOAC International; 2000.
Domínguez R., Pateiro M., Munekata P.E., Zhang W., Garcia-Oliveira P., Carpena M., Lorenzo J.M. Protein oxidation in muscle foods: A comprehensive review. Antioxidants. 2021; 11(1): 60.
Ozdemir V.F., Kocyigit R., Yanar M., Aydin R., Diler A., Palangi V., Lackner M. An investigation of slaughter weight and muscle type effects on carcass fatty acid profiles and meat textural characteristics of young Holstein Friesian bulls. Heliyon. 2024; 10(6).
Simitzis P.E. Enrichment of animal diets with essential oils—a great perspective on improving animal performance and quality characteristics of the derived products. Medicines. 2017; 4(2): 35.
Eburu P.O. Comparative evaluation of the effects of whole essential oils and their active constituent compounds on the biohydrogenation of polyunsaturated fatty acids and fermentation characteristics of rumen microbes in vitro (Doctoral dissertation). University of Essex; 2016.
Yang W.Z., Benchaar C., Ametaj B.N., Chaves A.V., He M.L., McAllister T.A. Effects of garlic and juniper berry essential oils on ruminal fermentation and on the site and extent of digestion in lactating cows. Journal Dairy Science. 2007; 90(12): 5671-5681.
Cobellis, G., Trabalza-Marinucci, M., Yu, Z. Critical evaluation of essential oils as rumen modifiers in ruminant nutrition: A review. Science of the Total Environment. 2016; 545, 556-568.
Pangesti, R.T., Jayanegara, A., Laconi, E.B. Effects of level and type of essential oils on rumen methanogenesis and fermentation: A meta-analysis of in vitro experiments. J. Anim. Feed. Sci. 2024; 33(3), 263-269.
Ribeiro A.D.B., Ferraz M.V.C., Polizel D. M., Miszura A. A., Barroso J. P.R., Cunha A.R., Pires A.V. Effect of thyme essential oil on rumen parameters, nutrient digestibility, and nitrogen balance in wethers fed high concentrate diets. Arq. Bras. Med. Vet. Zootec. 2020; 72(02): 573-580.
Fandiño I., Fernandez-Turren G., Ferret A., Moya D., Castillejos L., Calsamiglia S. Exploring additive, synergistic or antagonistic effects of natural plant extracts on in vitro beef feedlot-type rumen microbial fermentation conditions. Animals. 2020; 10(1): 173.
Valenzuela-Grijalva N.V., Pinelli-Saavedra A., Muhlia-Almazan A., Domínguez-Díaz D., González-Ríos H. Dietary inclusion effects of phytochemicals as growth promoters in animal production. Journal Anim. Science. Technol. 2017; 59(1): 8.
Yang W. Z., He M. L. Effects of feeding garlic and juniper berry essential oils on milk fatty acid composition of dairy cows. Nutr. Metab. Insights 2016; 9: NMI-S33395.
Hosoda K., Kuramoto K., Eruden B., Nishida T., Shioya S. The effects of three herbs as feed supplements on blood metabolites, hormones, antioxidant activity, IgG concentration, and ruminal fermentation in Holstein steers. Asian-Australas. J. Anim. Sci. 2005; 19(1): 35-41.
Bampidis V.A., Christodoulou V., Christaki E., Florou-Paneri P., Spais A. B. Effect of dietary garlic bulb and garlic husk supplementation on performance and carcass characteristics of growing lambs. Anim Feed Sci Technol. 2005; 121(3-4): 273-283.
Ma X., Li Y., Zhang S., Wang Y., Liu G. Effects of essential oils rich in cinnamaldehyde and carvacrol on growth performance and rumen fermentation in growing lambs. Anim. Feed Sci. Technol. 2024; 300: 115599.
Uyarlar C., Rahman A., Gultepe E. E., Cetingul I. S., Bayram I. Effect of a dietary essential oil blend in dairy cows during the dry and transition period on blood and metabolic parameters of dams and their calves. Animals. 2024; 14(1): 150.
Okoruwa M. I., Edoror O. M. Effect of garlic oil supplementation on intake, digestibility, performance and rumen function of goats fed silage based-diet. 2019.
de Jesus Ferreira F., das Dores Fernandes L., Júnior A. R. L., Rosado G. L., Bento C. B. P. Meta-analysis of the effects of essential oils on consumption, performance, and ruminal fermentation of beef cattle. Anim. Feed Sci. Technol. 2024; 311: 115956.
Leal K. W., Leal M. L., Breancini M., Signor M. H., Vitt M. G., Silva L. E. L., Da Silva A. S. Essential oils and capsaicin in the diet of Jersey cows at early lactation and their positive impact on anti-inflammatory, antioxidant and immunological responses. Trop. Anim. Health Prod. 2024; 56(7): 247.
Jaiswal L., Ismail H., Worku M. A review of the effect of plant-derived bioactive substances on the inflammatory response of ruminants (sheep, cattle, and goats). Int. Journal Vet. Anim. Med. 2020; 3: 130.
Rezaei M., Mohammadi A., Hosseinzadeh M. Effects of chamomile (Matricaria chamomilla L.) essential oil supplementation on blood parameters and immune response in ruminants: A review. Anim. Feed Sci. Technol. 2023; 295: 115543.
Zhang Y., Wang X., Liu J. Effects of dietary supplementation with essential oils on hematological parameters and immune response in dairy cows. J. Dairy Sci. 2020; 103(8): 7205–7216.
Yu Z., Wang Y., Dong B. Effects of thymol supplementation on rumen fermentation parameters and microbial populations in goats: an in vitro study. Front. Vet. Science. 2020; 7: 512.
Ramos-Morales E., Chaucheyras-Durand F., Newbold C. J. Role of essential oils in rumen fermentation and protein metabolism: practical implications for ruminants. Anim. 2022; 16(6): 100608.
BenJemaa M., Lassoued N., Omri B., Attia H. Effects of Thymbra capitata essential oil on rumen fermentation and microbial population in batch cultures. J. Appl. Microbiol. 2023; 134(2): 765-777.
Alabi J.O., Okedoyin D.O., Anotaenwere C. C., Wuaku M., Gray D., Adelusi O.O., Anele U.Y. Essential oil blends with or without fumaric acid influenced in vitro rumen fermentation, greenhouse gas emission, and volatile fatty acids production of a total mixed ration. Ruminants. 2023; 3(4): 373-384.
Roy B., Wahid H., Das S. Effects of thyme oil supplementation on rumen fermentation and ammonia nitrogen concentration in buffalo rumen liquor. Journal Vet. Science. 2015; 16(4): 441-450.
Foggi M., Rossi F., Bianchi R. In vitro evaluation of carvacrol and thymol on ruminal ammonia production and peptide degradation. Anim. Feed Sci. Technol. 2024; 290: 115568.
Marcel M., Oliveira D., Silva R., Cardoso M. Carcass characteristics, meat quality, and composition of lambs finished on pasture. Ciênc. Tecnol. Aliment. 2011; 31(4): 844-851.
Kim J.H., Kim C.H., Ko Y.D. Influence of dietary addition of dried Wormwood (Artemisia sp.) on the performance and carcass characteristics of Hanwoo Steers and the nutrient digestibility of sheep. National Livestock Research Institute, Rural Development Administration, Suweon; 2001.
Karami M., Alimon AR., Sazili A.Q., Goh Y.M. Meat quality and lipid oxidation of infraspinatus muscle and blood plasma of goats under dietary supplementation of herbal antioxidants. J. Anim. Vet. Adv. 2010; 9(24): 3039-3047.
Ornaghi M.G., Passetti R.A., Torrecilhas J.A., Mottin C., Vital A.C.P., Guerrero A., Prado I.N. Essential oils in the diet of young bulls: Effect on animal performance, digestibility, temperament, feeding behaviour and carcass characteristics. Anim. Feed Sci. Technol. 2017; 234: 274-283.
Alirezalu K., et al. Effects of herbal extracts on meat quality and shelf life: A review. Food Sci. Nutr. 2021; 9(4): 2145-2159.
Castellanos E., et al. Administration of distillate thyme leaves into the diet of Segureña ewes: Effect on lamb meat quality. Meat Sci. 2012; 92(4): 781-787.
Ünlü H.B., Ipçak H.H., Kandemir Ç. Effects of oregano essential oil and capsicum extract supplementation on lamb slaughter characteristics, meat quality, and fatty acid composition. S. Afr. J. Anim. Sci. 2024; 52(6).
Kahraman Çetin H., et al. Effects of supplementation of lamb rations with oregano essential oil on performance, antioxidant metabolism, and meat oxidative stability. Kafkas Univ. Vet. Fak. Dergisi. 2017; 23(3): 395-401.
Wood J.D., Enser M., Fisher A.V., Nute G.R., Richardson R. I., Hughes S.I., Whittington F.M. Fatty acid composition of meat from ruminants and its nutritional significance. Meat Sci. 2008; 78(1-2): 68-80.
Díaz D., Cañeque V. A contribution of beef to human health: A review of the role of nutrients in beef and a comparison with other meats. Anim. 2016; 10(7): 1138-1147.
Ribeiro J.S., et al. Effective Strategies for Understanding Meat Flavor: A Review. Korean J. Food Sci. Anim. Resour. 2024; 45(1): 165-182.
Kumar P., Sethi S. Development of a method suitable for high-throughput screening of antioxidant activity using linoleic acid emulsion and TBARS assay. Antioxidants. 2019; 8(12): 638.
Xia X., Zhang Y., Zhou G., Liu D. Influence of heating temperatures and storage on the odor of duck meat: Lipid oxidation and volatile flavor compounds. Food Chem. 2024; 422: 136148.
Deng B., Fu X., Sun Q., Wang Y. Changes in meat quality and volatile flavor compounds profile in dry-aged beef. Meat Sci. 2024; 201: 109732.
Falowo A. B. Antioxidant status of thyme and rosemary leaf powders and their effect on lipid oxidation of minced meat during cold storage. Biotech Stud. 2025; 34(1): 34-39.
Oyeyinka S. A., Afolayan A. J. Antioxidant status of thyme and rosemary leaf powders and their impact on lipid oxidation in minced beef during refrigerated storage. Biotech Stud. 2022; 34(1): 34-47.
Yildirim E., Eren M., Celik T., Kursun O., Çakmak O., Başaran N., Kaya S., Başaran A. A. Effects of dietary thyme and rosemary essential oils on biochemical parameters, antioxidant metabolism, and intestinal morphology in broilers. Poult. Sci. 2023; 102(5): 102023.
Khamisabadi, H. The Effect of Adding Peppermint or Thyme to the Diet on Fattening Performance and Meat Quality of Sanjabi Fattening Lambs. Final report of project, Agricultural Research, Education and Extension Organization (AREEO). 2019; FIPAC System Accession Number: 55770.
Khamisabadi, H. (2026). Effects of Dietary Peppermint and Thyme Powders on Growth, Immunity, Rumen Fermentation, Carcass, and Meat Quality in Sanjabi Fattening Lambs. Journal of Medicinal plants and By-products, 15(2), 290-297. doi: 10.22034/jmpb.2025.370516.2039
MLA
Khamisabadi, H. . "Effects of Dietary Peppermint and Thyme Powders on Growth, Immunity, Rumen Fermentation, Carcass, and Meat Quality in Sanjabi Fattening Lambs", Journal of Medicinal plants and By-products, 15, 2, 2026, 290-297. doi: 10.22034/jmpb.2025.370516.2039
HARVARD
Khamisabadi, H. (2026). 'Effects of Dietary Peppermint and Thyme Powders on Growth, Immunity, Rumen Fermentation, Carcass, and Meat Quality in Sanjabi Fattening Lambs', Journal of Medicinal plants and By-products, 15(2), pp. 290-297. doi: 10.22034/jmpb.2025.370516.2039
CHICAGO
H. Khamisabadi, "Effects of Dietary Peppermint and Thyme Powders on Growth, Immunity, Rumen Fermentation, Carcass, and Meat Quality in Sanjabi Fattening Lambs," Journal of Medicinal plants and By-products, 15 2 (2026): 290-297, doi: 10.22034/jmpb.2025.370516.2039
VANCOUVER
Khamisabadi, H. Effects of Dietary Peppermint and Thyme Powders on Growth, Immunity, Rumen Fermentation, Carcass, and Meat Quality in Sanjabi Fattening Lambs. Journal of Medicinal plants and By-products, 2026; 15(2): 290-297. doi: 10.22034/jmpb.2025.370516.2039