Anti-Catalyze Enzyme, Anti-biofilm, Antibacterial Effectiveness of Calcium Bio-Synthesized Nanoparticles from Pseudomonas aeruginosa

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Document Type : Research Paper

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Department of Biology, College of Education, Quran, University of Basrah, Basrah, Iraq

Abstract

Nanotechnology is a crucial modern science focused on the creation of small-sized particles with significant effectiveness, particularly in health applications. There are three primary methods for synthesizing nanoparticles: physical, chemical, and biological. The biological method is preferred due to its environmentally friendly nature, low cost, and minimal equipment requirements. This study utilized the biological method to synthesize calcium nanoparticles (CaNPs) using the supernatant solution from Pseudomonas aeruginosa bacteria isolated from soil. The nanoparticles were characterized microscopically, morphologically, and molecularly. Characterization techniques included colorimetric analysis, UV-visible spectrophotometry, FT-IR, and scanning electron microscopy (SEM), all of which confirmed the successful synthesis of CaNPs. Biofilm-forming bacteria were identified using Congo red and microtiter plate methods, revealing that the pathogenic isolates E. coli, K. pneumoniae, P. aeruginosa, and S. aureus exhibited a strong ability to form biofilms. Additionally, the effectiveness of the synthesized nanoparticles in inhibiting biofilms formed by these pathogenic bacteria was tested, demonstrating their antibacterial properties and ability to inhibit catalase enzyme activity.

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Journal of Medicinal plants and By-products

Articles in Press, Accepted Manuscript
Available Online from 23 April 2026

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