Antioxidant and Enzyme Inhibitory Activities of Ethanol Extracts of Selected Medicinal Plants from South Punjab, Pakistan

Manzoor, Rizwana; Azher, Iqbal; Qadir, Muhammad Imran; Saher, Tayyaba; Mudassir, Jahanzeb; Dameesha, Dameesha; Hasnain, Syed Zia Ul; Aleem, Ambreen; Abbas, Khizar

doi:10.22034/jmpb.2026.370481.2040

Antioxidant and Enzyme Inhibitory Activities of Ethanol Extracts of Selected Medicinal Plants from South Punjab, Pakistan

Articles in Press

Document Type : Research Paper

Authors

¹ Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Pakistan

² Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan

³ Institute of molecular biology and biotechnology, Bahauddin Zakariya University, Multan, Pakistan

⁴ Department of Pharmacognosy, Faculty of Pharmacy, Bahauddin zakariya University

10.22034/jmpb.2026.370481.2040

Abstract

Natural products are promising source of drugs in pharmaceutical industry, used for the treatment of diseases and serves as a lead molecule for the synthesis of potent drugs. Therefore, current research work was planned to investigate in-vitro antioxidant, antidiabetic and hypolipidemic potential. For this purpose, different parts of Albizia lebbeck L., Mangifera indica L., Vitis vinifera L., Syzigium jabolanum L., Abelmoschus esculentus L., Calotropis procera L., Grewia asiatica L., Morus alba L., and Leucaena leucophala L. were collected from South Punjab. After collection, these parts were dried and extraction was performed by maceration. Total phenolics and total flavonoids contents were measured. Antioxidant potential, in-vitro α-glucosidase, α- amylase, lipase, lipoxygenase and urease inhibition assay was performed. Result showed that Calotropis procera (leaves) had maximum phenolic content of 66 mg/g equivalent of gallic acid and had maximum flavonoid content of 76mg/g equivalent of quercetin. Ficus carica (leaves) showed maximum antioxidant potential of 86%. Vitis vinifera (leaves) showed inhibitory concentration of 50 for lipoxygenase assay. Abelmoschus esculentus (aerial) showed maximum percentage inhibition of 87% for α-glucosidase inhibition assay. Vitis vinifera (leaves) showed maximum inhibition of 79% for α- amylase inhibition assay. Calotropis procera (leaves) had maximum percentage inhibition of 83% for lipase assay. Abelmoschus esculentus (aerial part) showed inhibitory concentration of 37 for urease assay. It is concluded from the aforementioned results that Calotropis procera leaves showed strong DPPH inhibition, lipase inhibition and urease inhibition potential. Vitis vinifera leaves had significant lipoxygenase inhibition, lipid modulating and antidiabetic potential whereas Calotropis procera leaves possessed promising lipid-modulating properties. Therefore, these plants may be helpful in the development of novel therapeutic approaches for dyslipidemia and related metabolic disorders.

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