Screening and Optimization for Smyrnium cordifolium Boiss: Extraction, Isolation, and Identification of Bioactive Components

Document Type : Research Article

Authors

Department of Food Science and Technology, Ro.C., Islamic Azad University, Roudehen, Iran

Abstract

Smyrnium cordifolium Boiss is considered a medicinal plant of Apiaceae family, which has a bright future in the identification of bioactive components and the fields of medicine. The present research aimed to optimize the extraction of S. cordifolium leaves using ultrasound-assisted extraction and determine phytochemical constituents, total phenolic content and antioxidant attributes. Initially, the effective factors on extraction were screened in eight examined assays through the Taguchi design approach. The best conditions were as follows in the present research: 80% ethanol concentration, 20 °C temperature, 200 W ultrasound power, 30 min period, young leaves and ratio of extract to solvent 1:60. The most suitable responses were hexanoic acid (8.96 mg/g), 2-methylhexanoic acid (8.22 mg/g), 8-quinolinol-2-butyl (9.99 mg/g), octadecanoic acid (10.22 mg/g) and phytol (34.3 mg/g) using the high-performance liquid chromatography. Furthermore, TPC (48.25 mg gallic acid/g) and antioxidant traits (73.26%) were calculated for the optimized sample. The ultrasound power, solvent concentration, and leaf age were screened as the most effective factors for optimization by the central composite design (CCD). The levels of 8.46, 6.78, 10.15 and 11.24 mg/g for hexanoic acid, 2-methylhexanoic acid, 8-quinolinol-2-butyl and octadecanoic acid were predicted based on CCD in twenty samples and conditions of 43.7% ethanol, 97.99 W ultrasound power, and 56 days old leaves, respectively. The TPC (43.97 mg gallic acid/g) content and antioxidant activity (63.68%) were reported for the optimized treatment. According to the results obtained, S. cordifolium extract has suitable phytochemical components that possess the necessary potential to play a functional role.

Keywords

Main Subjects

References

  1. Aboonajmi M., Kakelahi A., Arabhosseini A., Sadat-Noori S.A. Efficacy of ultrasonic waves in extraction ascorbic acid and bioactive compounds from Ajowan seeds (Carum copticum L.) and comparison with conventional Soxhlet extraction. Journal of Medicinal Plants and By-Products. 2024;13(4):1159–1165. doi: 10.22034/jmpb.2024.363314.1596.
  2. Doodman S., Saeidi K., Lorigooini Z., Kiani M. Chemical composition of essential oils from Smyrnium cordifolium Boiss. (Apiaceae) ecotypes. Biochemical Systematics and Ecology. 2023;110:104682. doi:10.1016/j.bse.2023.104682.
  3. Taylor J.R.N., Graaff T., David J., Kamau D., Joshi D., Byinshi B., De Kock H.L. Vitamins are retained in fortified whole-grain maize meal when stored under tropical conditions when optimal storage practices are used. Cereal Chemistry. 2025;102(5):894–908. doi:10.1002/cche.70006.
  4. Miri S.M., Ahmadi S., Moradi P. Influence of salicylic acid and citric acid on the growth, biochemical characteristics and essential oil content of thyme (Thymus vulgaris L.). Journal of Medicinal Plants and By-Products. 2015;4(2):141–146.
  5. Oreopoulou A., Tsimogiannis D., Oreopoulou V. Extraction of polyphenols from aromatic and medicinal plants: An overview of the methods and the effect of extraction parameters. In: Watson R.R., editor. Polyphenols in Plants. 2nd ed. Academic Press. 2019;243–259. doi:10.1016/B978-0-12-813768-0.00025-6.
  6. Alkhamessi R.N., Abbas M.I., Bahedh S.B., Kadham M.J., Lahhob Q.R., Mudhafar M. Harnessing medicinal plants and their by-products to combat fungal pathogens in the face of climate change: Implications for global food security. Journal of Medicinal Plants and By-Products. 2026;15(1):102–110. doi:10.22034/jmpb. 2025.369007.1934.
  7. Ashengroph M., Nahvi I., Amini J. Application of Taguchi design and response surface methodology for improving conversion of isoeugenol into vanillin by resting cells of Psychrobacter sp. CSW4. Iranian Journal of Pharmaceutical Research. 2013;12(3):411–421. doi:10.22037/ijpr.2013.1337.
  8. Sodeifian G.H., Azizi J., Ghoreishi S.M. Response surface optimization of Smyrnium cordifolium Boiss. (SCB) oil extraction via supercritical carbon dioxide. The Journal of Supercritical Fluids. 2014;95:1–7. doi:10.1016/j.supflu.2014.07.023.
  9. Tabaraki R., Ghadiri F. In vitro antioxidant activities of aqueous and methanolic extracts of Smyrnium cordifolium Boiss. and Sinapis arvensis L. International Food Research Journal. 2013;20(5):2111–2115.
  10. Muñoz-Labrador A., Prodanov M., Villamiel M. Effects of high intensity ultrasound on disaggregation of a macromolecular procyanidin-rich fraction from Vitis vinifera L. seed extract and evaluation of its antioxidant activity. Ultrasonics Sonochemistry. 2019;50:74–81. doi:10.1016/j.ultsonch.2018.08.030.
  11. Hęś M., Dziedzic K., Górecka D., Jędrusek-Golińska A., Gujska E. Aloe vera (L.) Webb.: Natural sources of antioxidants – A review. Plant Foods for Human Nutrition. 2019;74(3):255–265. doi:10.1007/s11130-019-00747-5.
  12. Eom S.J., Kim Y.E., Kim J.E., Park J., Kim Y.H., Song K.M., Lee N.H. Production of Undaria pinnatifida sporophyll extract using pilot-scale ultrasound-assisted extraction: Extract characteristics and antioxidant and anti-inflammatory activities. Algal Research. 2020;51:102039. doi:10.1016/j.algal.2020.102039.
  13. Đurović S., Nikolić B., Luković N., Jovanović J., Stefanović A., Šekuljica N., Knežević-Jugović Z. The impact of high-power ultrasound and microwave on the phenolic acid profile and antioxidant activity of the extract from yellow soybean seeds. Industrial Crops and Products. 2018;122:223–231. doi:10.1016 /j.indcrop.2018.05.078.
  14. Sharma P., Wichaphon J., Klangpetch W. Antimicrobial and antioxidant activities of defatted Moringa oleifera seed meal extract obtained by ultrasound-assisted extraction and application as a natural antimicrobial coating for raw chicken sausages. International Journal of Food Microbiology. 2020;332:108770. doi:10.1016/j.ijfoodmicro.2020.108770.
  15. Savic I.M., Savic Gajic I.M. Optimization of ultrasound-assisted extraction of polyphenols from wheatgrass (Triticum aestivum L.). Journal of Food Science and Technology. 2020;57(8):2809–2818. doi:10.1007/s13197-020-04312-w.
  16. Chen X.M., Ma Z., Kitts D.D. Effects of processing method and age of leaves on phytochemical profiles and bioactivity of coffee leaves. Food Chemistry. 2018;249:143–153. doi:10.1016/j. foodchem.2017.12.073.
  17. Ghazy O.A., Fouad M.T., Saleh H.H., Kholif A.E., Morsy T.A. Ultrasound-assisted preparation of anise extract nanoemulsion and its bioactivity against different pathogenic bacteria. Food Chemistry. 2021;341:128259. doi:10.1016/j.foodchem. 2020.128259.
  18. Carrera C., Ruiz-Rodríguez A., Palma M., Barroso C.G. Ultrasound assisted extraction of phenolic compounds from grapes. Analytica Chimica Acta. 2012;732:100–104. doi:10.1016 /j.aca.2011.11.032.
  19. Dong R., Liu S., Xie J., Chen Y., Zheng Y., Zhang X., Yu Q. The recovery, catabolism and potential bioactivity of polyphenols from carrot subjected to in vitro simulated digestion and colonic fermentation. Food Research International. 2021;143:110263. doi:10.1016/j.foodres.2021.110263.
  20. Boussetta N., Soichi E., Lanoisellé J.L., Vorobiev E. Valorization of oilseed residues: Extraction of polyphenols from flaxseed hulls by pulsed electric fields. Industrial Crops and Products. 2014;52:347–353. doi:10.1016/j.indcrop.2013.10.048.
  21. Rajha H.N., Boussetta N., Louka N., Maroun R.G., Vorobiev E. A comparative study of physical pretreatments for the extraction of polyphenols and proteins from vine shoots. Food Research International. 2014;65:462–468. doi:10.1016/j.foodres.2014.04. 024.
  22. Nouri M. Nanoemulsions of Trachyspermum copticum L. and Teucrium polium L. essential oils incorporated with coating to extend fillet chicken storage. Journal of Food Measurement and Characterization. 2024;18(6):4034–4046. doi:10.1007/s11694-024-02474-1.
  23. Nobossé P., Fombang E.N., Mbofung C.M. Effects of age and extraction solvent on phytochemical content and antioxidant activity of fresh Moringa oleifera L. leaves. Food Science & Nutrition. 2018;6(8):2188–2198. doi:10.1002/fsn3.783.
  24. Anand S., Sonar R.M., Jain K. Traditional knowledge research in India: A bibliometric-based review and thematic analysis. Indian Journal of Traditional Knowledge. 2025;24(3):275–287. doi:10.56042/ijtk.v24i3.9491.
  25. Pandikumar P., Mutheeswaran S., Chelladurai V., Ignacimuthu S. Notes on traditional method of solidified Aloe vera exudate (musabhar) preparation in Tamil Nadu: Preparation of musabhar. Indian Journal of Traditional Knowledge. 2025;24(8):796–798. doi:10.56042/ijtk.v24i8.15412.
  26. Mamati G.E., Liang Y., Lu J. Expression of basic genes involved in tea polyphenol synthesis in relation to accumulation of catechins and total tea polyphenols. Journal of the Science of Food and Agriculture. 2006;86(3):459–464. doi:10.1002/jsfa.2368.
  27. Semsarzadeh M., Sayfzadeh S., Darvishi Zeidabadi D., Zakerin H., Hadidi Masuleh I. The role of zeolite in mitigating physiological and biochemical stress responses and enhancing seed yield of coriander (Coriandrum sativum L.) under drought conditions. Journal of Medicinal Plants and By-products. 2025:1–9. doi:10.22034/JMPB.2025.369791.1996.
Journal of Medicinal plants and By-products
Volume 16, Issue 1
January and February 2027
Pages 102-111

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