Green Biosynthesis and Characterization of Pseudomonas mendocina-Derived Iron Nanoparticles: Antibacterial and Antibiofilm Activities Against Pathogenic Bacteria

Abas, Ismaal Jmia; Al-Kaaby, Israa Najim Abdullah; Shaban, Abbas Dareb

doi:10.22034/jmpb.2027.373803.2246

Green Biosynthesis and Characterization of Pseudomonas mendocina-Derived Iron Nanoparticles: Antibacterial and Antibiofilm Activities Against Pathogenic Bacteria

Articles in Press

Document Type : Research Article

Authors

Department of Biology, College of Education-Al-Qurna, Basrah University, Basrah, Iraq

10.22034/jmpb.2027.373803.2246

Abstract

The increasing prevalence of antimicrobial resistance and biofilm-forming bacteria has encouraged the development of alternative antimicrobial agents. This study aimed to biosynthesize iron nanoparticles (FeNPs) using the cell-free supernatant of Pseudomonas mendocina, characterize the synthesized nanoparticles, and evaluate their antibacterial and antibiofilm activities against Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus. The biosynthesized FeNPs were characterized by UV–Visible spectroscopy, FT-IR, and SEM. Antibacterial activity was evaluated using the agar well diffusion method, while MIC, MBC, and antibiofilm activity were determined using standard microbiological assays. The FeNPs exhibited concentration-dependent antibacterial activity against all tested pathogens, with P. aeruginosa showing the highest susceptibility and K. pneumoniae the lowest. The MIC was 25 µg/mL for all isolates, whereas the MBC was 75 µg/mL. In addition, the nanoparticles significantly inhibited biofilm formation in all tested bacteria. These findings indicate that biosynthesized FeNPs from Pseudomonas mendocina possess promising antibacterial and antibiofilm properties and may serve as eco-friendly nanomaterials for combating biofilm-associated bacterial infections.

Keywords

Main Subjects