Effect of Solvent Type on Rosmarinic Acid, Total Phenol, Flavonoids, and Antioxidant Activity of Nepeta asterotricha Rech. f: An Endemic Plant from Iran

Document Type : Research Paper

Authors

1 Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran

2 Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran

3 Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, (Mahan Golsay Company, Yazd) Iran

Abstract

The current study aims to investigate the effect of solvent variety on some phytochemical factors of Nepeta asterotricha Rech. f.: as an endemic plant from Iran. It is suspected that quantity and quality of chemical compounds would be affected by solvent type. For this purpose, aqueous, hydro-alcohol (50/50 ethanol/water), and methanol were used as solvents. The studied factors were total phenol, flavonoid, antioxidant activity, and rosmarinic acid that were measured by Folin-Ciocalteu, colorimetric, FRAP, and HPLC methods, respectively. The outcomes showed hydro-alcohol extraction could significantly isolate phenol compounds (156.84 mg GAE/g DW) with considerable antioxidant properties (318.55 mg AA/g DW). However, methanol was more effective to extract total flavonoid content (101.34 mg RE/g DW) and rosmarinic acid (140.39% DW). In addition, more study should be done to investigate the importance of each compounds for both medicinal and industrial uses.

Keywords

References

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Journal of Medicinal plants and By-product
Volume 10, Special
April 2021
Pages 89-93

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