Study of Essential Oil and Effect of Temperature and Seed Appendages (pappus) on Germination Characteristics in Coltsfoot

Document Type : Research Paper

Authors

Department of Horticultural Science, Tarbiat Modares University, Tehran, Iran

Abstract

Coltsfoot (Tussilago farfara L.) is a perennial plant of the Asteraceae family that has been used as an ancient folk remedy to treat coughs, bronchitis, and asthmatic disorders. Ripe seeds were collected from T. farfara plants growing in Pol-e Zangholeh, Iran, in this study. Samples were gathered by the Tarbiat Modares University (TMU) Department of Horticultural Sciences laboratory in late April 2016. The extracted seed essential oil constituents identified and quantified using GC/MS and GC, respectively. Phy,tol, n-Nonadecane, n-Tetradecane, and 4,4-Dimethyltetracyclo [5.2.1.02,6.03,5] decane, with amounts of 30.5%, 11.4%, 9.8%, and 6.2%, respectively, were the main components in the seeds oil of T. farfara. The germination variables were studied using a factorial experiment with 5 replicates × 50 seeds in a totally randomized design. Treatments include: cultivating non-pappus-bearing seeds in a Petri dish and placing them at 4°C (c1t1) and 25 °C (c1t2), Cultivating pappus-bearing seeds in a Petri dish and keeping them at 4 °C (c2t1) and 25 °C (c2t2) and cultivating of non-pappus-bearing seeds treated with sodium hypochlorite 1.5% for 20 minutes and keep them at 4 °C (c3t1) and 25 °C (c3t2). The interaction between culture type and temperature was significant (p≤0.01) for the germination percentage of seeds, germination rate, and seed vigor of T. farfara, according to analysis of variance. The rising seed germination rates of C3t2 and C3t1 were reported at 76.4% and 74.8%, respectively. The results revealed the closest positive correlation between germination rate, vigor index, and seed germination percentage (p≤0.001).

Keywords

Main Subjects

References

  1. Song K., Lee K.J., Kim Y.S. Development of an efficient fractionation method for the preparative separation of sesquiterpenoids from Tussilago farfara by counter-current chromatography. J. Chromatogr. A. 2017;1489: 107-114.
  2. Norani M., Ebadi M.-T., Ayyari M. Volatile constituents and antioxidant capacity of seven Tussilago farfara L. populations in Iran. Sci. Hortic. 2019;257:p108635.
  3. Pfeiffer T., Günzel C., Frey W. Clonal reproduction, vegetative multiplication and habitat colonisation in Tussilago farfara (Asteraceae): a combined morpho-ecological and molecular study. Flora: Morphol. Distrib. Funct. Ecol. 2008; 203(4):281-291.
  4. Xu J., Kang J., Liu F., Wang P., Ma J., Zhou H., Jin D., Ohizumi Yasushi., Lee D., Bartlam M., Guo Y. Chemical and biological profiles of Tussilago farfara: Structures, nitric oxide inhibitory activities, and interactions with iNOS protein. J. Funct. Foods. 2017;32:37-45.
  5. Qu H., Yang W., Li J. Structural characterization of a polysaccharide from the flower buds of Tussilago farfara, and its effect on proliferation and apoptosis of A549 human non-small lung cancer cell line. Int. J. Biol. Macromol. 2018;113:849-858.
  6. Judzentiene A. Budiene J. Volatile oils of flowers and stems of Tussilago farfara L. from Lithuania. J. Essent. Oil-Bear. Plants. 2011;14(4):413-416.
  7. Keiffer C.H. Ungar I.A. The effect of extended exposure to hypersaline conditions on the germination of five inland halophyte species. Am. J. Bot. 1997;84(1):104-111.
  8. Rathinavel K., Dharmalingam C. Efficacy of seed treatment on storability of cotton seeds and seedling vigour. J. Trop. Agric. 2006;39(2):128-133.
  9. Lan Y., et al. Effects of pappus removal and low-temperature short-term storage on interspecific and intraspecific variation in seed germination of Luobuma. Seed Science and Technology, 2019;47(1):13-24.
  10. Nykiforuk C.L., Johnson-Flanagan A.M. Germination and early seedling development under low temperature in canola. Crop Sci. 1994;34(4):1047-1054.
  11. Norsazwan M., et al. Temperature fluctuation improves oil palm (Elaeis guineensis) dura× pisifera seed germination. Seed Science and Technology, 2020. 48(1): 49-55.
  12. Mairesse L.A.S., Costa E.A., Farias J.R., Rubens A.F. Bioatividade de extratos vegetais sobre alface (Lactuca sativa L.). Rev. FZVA. 2007;14(2).
  13. Ranil R., Niran M., Plazas R.M., Fonseka H.H., Fonseka D, Vilanova S., Andújar I., Gramazio P., Fita A., Prohens J. Improving seed germination of the eggplant rootstock Solanum torvum by testing multiple factors using an orthogonal array design. Sci. Hortic. 2015;193:174-181.
  14. Lima J.d.S., Eduardo F.A., Roberto F.A., Santos Dias A., Santos Dias C.F., Rena F.C. Uso da reidratação e do hipoclorito de sódio para acelerar a emergência de plântulas de cafeeiro. Rev. bras. sementes. 2012;34(2): 327-333.
  15. Jesus V.A.M.d., Nóbrega E.E., Nóbrega E.E., Neves A., Silva R.F. Roberto Ferreira da Silva. Ratio of seeds and sodium hypochlorite solution on the germination process of papaya seeds. J. Seed Sci.. 2016;38(1):57-61.
  16. Pharmacopoeia B., Commission B.P. The stationery office. London, UK 2009;1:123-128.
  17. Adams R.P. Identification of essential oil components by gas chromatography/mass spectrometry. Vol. 456. 2007: Allured publishing corporation Carol Stream.
  18. Irvani N., Solouki M., Omidi M., Zarekarizi A. Seed germination and dormancy breaking in dorema ammoniacum D., an endangered medicinal plant. TJS. 2012;10(1):9-15.
  19. Maguire J.D., Speed of germination-Aid in selection and evaluation for seedling emergence and vigor 1. Crop science, 1962;2(2):176-177.
  20. Abdul-Baki A.A., Anderson J.D. Vigor determination in soybean seed by multiple criteria. Crop Sci. 1973; 13(6): 630-633.
  21. Pérez-García L.-A., Sáenz-Mata J., Manuel F.H., Navarro-Muñoz C. E., Palacio-Rodríguez R., Preciado-Rangel P. Plant-growth-promoting rhizobacteria improve germination and bioactive compounds in cucumber seedlings. Agron. 2023; 13(2): 315.
  22. Ferrer D.B., Venskutonis P.T., Talou T., Zebib B., Ferrer J.M.B., Merah O. Potential interest of Tussilago farfara (L.) whole plant of Lithuanian and French origin for essential oil extraction. Am. J. Essent. Oil. Nat. Prod. 2016;12-15.
  23. Liu L.-L., Yang J.-L., Shi Y.-P. Sesquiterpenoids and other constituents from the flower buds of Tussilago farfara. J. Asian Nat. Prod. Res. 2011; 13(10): p. 920-929.
  24. Khatibzadeh R., Azizi , Arouiee H., Farsi M. Effects of sterilization protocol and pre-chilling treatment on in vitro seed germination of levisticum officinale koch. Journal of horticulture science ( J. Agric. Sci. Technol.). 2013;27(2):130-138.
  25. Nosratti I., Abbasi R., Bagheri A., Bromandan P. Seed germination and seedling emergence of Iberian starthistle (Centaurea iberica). Weed Biol. Manag. 2017;17(3):144-149.
  26. Gorai M., Gasmi H., Neffati M. Factors influencing seed germination of medicinal plant Salvia aegyptiaca L.(Lamiaceae). Saudi J. Biol. Sci.. 2011;18(3):255-260.
  27. Babadoost M., Derie M., Gabrielson R. Efficacy of sodium hypochlorite treatments for control of Xanthomonas campestris pv. campestris in Brassica seeds. SST. 1996;24(1):7-15.
  28. Sharma R.K., Sharma S., Sharma S.S. Seed germination behaviour of some medicinal plants of Lahaul and Spiti cold desert (Himachal Pradesh): implications for conservation and cultivation. Curr. Sci. 2006;1113-1118.
Journal of Medicinal plants and By-Products
Volume 13, Issue 3
September 2024
Pages 538-544

Files

Share

How to cite

Statistics