Physiological Responses of Melissa the Effects of Growth Regulators on Induction of Callus Tissue and Regeneration of Harmala (Peganum harmala L.)

Document Type : Research Paper

Authors

Department of Agricultural Sciences, Payame Noor University, Tehran, Iran

Abstract

There has been limited information on the in-vitro propagation of Harmal. In this research, leaf, hypocotyl and embryo samples of this plant were examined for callus formation and regeneration. The present experiment was conducted as factorial based on a completely randomized design with three replications in two separate experiments with different hormonal treatments. In the first experiment, MS medium contained growth with different concentrations of Benzylaminopourine ((BAP) 0, 0.5, 1 and 2 mg/L) in combination with Naphthalene acetic acid (NAA) at concentrations (0, 0.25 and 0.5 mg/L. In the second experiment, BAP with concentrations (0, 0.5, 1 and 2 mg/L) in combination with 2,4-Dichlorophenoxyacetic acid (2, 4-D) with concentrations (0, 25 and 0.5 mg / L). The results of the first experiment showed that the highest callus and regeneration for the Harmal a plant was the combination of BAP hormone (0.5 mg/L) with NAA (0.5 mg/L). In this research, leaf, hypocotyl and embryo samples of this plant were examined for callus formation and regeneration. The results of the second experiment, on the other hand, showed that callus of explants was carried out in most of the BAP and 2,4-D hormonal treatments, and the highest regeneration was observed in the treatment of 1 mg/L BAP with 0.5 mg/L 2,4-D. High BAP concentration in the presence of NAA decreased callus formation and appeared to be better for NAA callus enhancement. The high concentration of BAP in the presence of NAA reduced callus formation and it can be said that NAA was better for increasing callus production. It was found that in the presence of 2,4-D and BAP, leaf explants were more efficient for regeneration and callus formation compared to hypocotyl and embryo explants. In the medium supplemented with 0.5 mg/L 2,4-D and 1 mg/L BA for the leaf and hypocotyl explants, Maximum callus induction and shoot regeneration was obtained.

Keywords

Main Subjects

References

  1. Asgharpanah A., Ramezanloo E. Plant regeneration from hypocotyl culture of Peganum harmala, Pakistan J. Botany. 2012;34:253-256.
  2. Sharifi A., Hashemi-Sohi H., Azadi P., Sharifi, A.A. Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi. Physiological Molecular Biology of Plants. 2014; 20(2):257-262.
  3. Mohammadi Q. River Mand of Bushehr: Bushehr University of Medical Sciences Publications.
  4. Rout G.R., Samantaray S., Das P. In vitro manipulation and propagation of medicinal plants. Biotechnology Advances. 2007;18:91–120.
  5. Sirvastava R. Studying the information needs of medicinal plant stakeholders in Europe. TRAFFIC Dispatches. 2000;15:5.
  6. Rout G.R., Samantaray S., Das P. In vitro manipulation and propagation of medicinal plants. Biotechnology Advances. 2000;18:91–120.
  7. Kumar J., Kumari B.R., Castano E. Cyclic somatic embryogenesis and efficient plant regeneration from callus of safflower. Biologia Plantarum. 2008; 52(3):429-436.
  8. Mandal A., Gupta S. Somatic embryogenesis of safflower: influence of auxin and ontogeny of somatic embryos. Plant Cell, Tissue Organ Culture. 2003;72 (1):27-31.
  9. Ghasempour H., Soheilikhah Z., Zebarjadi A.R., Ghasempour S., Karimi N. In vitro micro propagation, callus induction and shoot regeneration in safflower L. Cv. Leaf. Iranian J Plant Physiol. 2014; 4(2):999- 1004.
  10. Huang J., Yang J., Guan L., Yi S., Du L., Tian H., Guo Y., Zhai F., Lu Z., Li H., Li X., Jiang C. Expression of bioactive recombinant human fibroblast growth factor 10 in Carthamus tinctorius L. Seeds. Protein Expression and purification. 2017; Vol.138:7-12.
  11. Patial V., Krishna R., Arya G., singh V.K., Agrawal M., Goel S., Jagannath A., Kumar A. Development of an effecient, genotype independent plant regeneration and transformation protocol using cotyledonary nodes in safflower (Carthamus tinctorius). J Plant Biochem & Biotech. 2016; 25(4):421-432.
  12. Basalma D., Uranbey S., Mirici S., Kolsarici O. TDZ×IBA induced shoot regeneration from cotyledonary leaves and in vitro multiplication in safflower (Carthamus tinctorius L.). African J Biotech. 2008;7(8):960-966.
  13. Nikhil M., Dudhare M.S., Jadhav P.V., Moharil M.P., Deshmukh AG. In vitro shoot regeneration plantlet development in safflower (Carthamus tinctorius). The bioscan. 2014;9(2):551-555.
  14. Wang X-D., Nolan K.E., Irwanto R.R., Sheahan M.B., Rose R.J. Ontogeny of embryogenic callus in Medicago truncatula: the fate of the pluripotent and totipotent stem cells. Annals of Botany. 2011;107(4):599-609.
  15. Hagimori M.T., Matsumoto T. Kiaski. Determination of digitoxin and digoxin contents in first and second passage calli and organ redifferentiating calli of several Digitalis species by radioimmunoassay. Plant Physiology. 1980; 21: 1391-1063.
  16. Smita R., Sangeeta R., Kumar S.S., Soumya S., Deepak P. An ethnobotanical survey of medicinal plants in Semiliguda of Koraput District, Odisha, India. Res J Recent Sci. 2012; 2(8): 20-30.
  17. Ziaratnia, S.M. Somatic embryogenesis in Buniumpersicum. Industrial and Scientific Research Organization of Iran, Khorasan Centre. 2000.
  18. Eari S., Aghdasi M., Ahmadzadeh E., Mianabadi M. Influence of Plant Growth Regulators on Callus Induction, Silymarin Production and Antioxidant Activity in Milk Thistle (Silybum marianum L. Gaertn.) under Tissue Culture Medium. J Med Plants & By-products. 2017;1:59-69.
  19. Otroshy M., Moradi K. Effect of explants and growth regulators on direct organogenesis of Dracocephalumkotschyi Boiss. via tissue culture technique. J. Herbal Drugs. 2012;3(3):127-134.
  20. Sharifi A., Hashemi-Sohi H., Azadi P., Sharifi A. A. Hairy root induction and plant regeneration of medicinal plant Dracocephalumkotschyi. Physiol Molecular Biology of Plants. 2014; 20(2):257-262.
  21. Mandal A., Gupta S. Somatic embryogenesis of safflower: influence of auxin and ontogeny of somatic embryos. Plant Cell, Tissue Organ Culture. 2003; 72 (1):27-31.
  22. Rahimi M., Daneshvar M. Microfilming of inverted tulip (Fritillaria imperialis L.) through indirect regeneration under in vitro culture. J. Med Plants (J. Agric. Sci). 2017; 39 (4):31-42.
  23. Alizadeh S., et. al. Interaction effect of growth regulators on callus production using Fritillariaimperialis L. J. Plant Production. 2016; 39 (2): 81-94.
  24. Fehér A., Pasternak T., Otvos K., Miskolczi P., Dudits D. Induction of embryogenic competence in somatic plant cells: a review. Biologia - Section Botany. 2002;57(1): 5-12.
  25. Murashige T., Skoog F. A revised medium for the rapid growth of and bioassays with tobacco tissue cultures. Physiol. Plant. 1962; 15: 473-479.
  26. Ren J.P., Wang X.G., Jun Y. Dicamba and sugar effects on callus induction and plant regeneration from mature embryo culture of wheat. Agric Sci in China. 2010;9(1): 31-37.
  27. Sidhu Y. In vitro micro propagation of medicinal plants by tissue culture. The Plymouth Student Scientist. 2010; 4: 432-449.
  28. Otroshy M., Moradi K. Effect of explants and growth regulators on direct organogenesis of Dracocephalumkotschyi Boiss. via tissue culture technique. J. Herbal Drugs. 2012;3(3):127-134.
  29. Fatima Z.A., Mojib S., Fatima A., Arshi S. Umar. Callus induction, biomass growth and regeneration in Digitalis lanataEhrh: influence of plant growth regulators and carbohydrates. J. Biotechnology. 2009;33:1-13.
  30. Murashige T., Skoog F. A revised medium for the rapid growth of and bioassays with tobacco tissue cultures. Physiol. Plant. 1962;15:473-479.
  31. Sales E.S.G., Nebaer I. Arrillaga and J. Segura. Plant hormones and Agrobacterium tumefaciens strain 82.139 induce efficient plant regeneration in the cardenolide- producing plant Digitalis Minor. J. Plant Physiology. 2002;159:9-16.
  32. Rashidi M., Saremi-Rad A., Froozesh P., Ghasemo Omran V.O. Effect of plant growth regulators on Fittonia verschaffeltii regeneration at in vitro conditions. J. Plant Physiology & Breeding. 2008;8(2):59-68.
  33. Sun Huh Y., Kwan Lee J., Young Nam S. Effect of plant growth regulators and antioxidants on in vitro plant regeneration and callus induction from leaf explants of purple passion fruit (Passifloraedulis Sims). J. Plant Biotechnol. 2017;44:335–342.
  34. Eari S., Aghdasi M., Ahmadzadeh E., Mianabadi M. Influence of Plant Growth Regulators on Callus Induction, Silymarin Production and Antioxidant Activity in Milk Thistle (Silybum marianum L. Gaertn.) under Tissue Culture Medium. J. Medicinal Plants and By-products. 2017; 1: 59-69.
  35. Machakova I., Zazimalova E., George E.F. Plant growth regulators I: introduction; auxins, their analogues and inhibitors. In: George EF, Hall MA and De Klerk GJ (eds.). Plant Propagation by Tissue Culture. 2008; Pp. 175-204. Springer.
Journal of Medicinal plants and By-Products
Volume 13, Issue 3
September 2024
Pages 551-559

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